有靶向非编码RNA的阳性对照siRNA吗?阴性对照呢?
我们有靶向MALAT-1的阳性对照siRNA(货号4455877),MALAT-1是一个主要位于细胞核内的非编码RNA。Silencer Select阴性对照1和2可以作为阴性对照。
我的Silencer或Silencer Select siRNA实验应该使用什么阳性和阴性对照?
对于Silencer和Silencer Select siRNA我们均提供多个阴性和阳性对照。
Silencer siRNA阳性对照有:
- Silencer Firefly Luciferase (GL2 + GL3) siRNA(货号AM4629, 5nmol)
- Silencer GAPDH siRNA(人)(货号AM4605, 5nmol; AM4633, 40 nmol)
- Silencer GAPDH siRNA (人,小鼠,大鼠) (货号AM4624, 5 nmol; AM4631, 40 nmol; AM4632, 5 x 40 nmol)
- Silencer GFP (eGFP) siRNA (货号AM4626, 5 nmol)
- Silencer KIF11 (Eg5) siRNA (人,小鼠,大鼠) (货号AM4639, 5 nmol)
如果使用荧光标记的Silencer siRNA,我们有:
- Silencer Cy-3标记GAPDH siRNA (人,小鼠,大鼠) (货号AM4649, 5 nmol)
- Silencer FAM-标记GAPDH siRNA(人,小鼠,大鼠) (货号AM4650, 5 nmol)
如果使用体内级别的Silencer siRNA,可以选:
- Silencer GAPDH阳性对照siRNA,in vivo ready(货号4404025, 250 nmol)
Silencer siRNA阴性对照有:
- Silencer阴性对照1号 siRNA(货号AM4611, 5 nmol; AM4635, 40 nmol; AM4636, 5 x 40 nmol)
- Silencer阴性对照2号siRNA(货号AM4613, 5 nmol; AM4637, 30 nmol)
- Silencer阴性对照3号 siRNA(货号AM4615, 5 nmol)
- Silencer阴性对照4号 siRNA(货号AM4641, 5 nmol)
- Silencer阴性对照5号 siRNA(货号AM4642, 5 nmol)
如果使用荧光标记的Silencer siRNA,我们有:
- SilencerCy 3-标记阴性对照1号siRNA(货号AM4621, 5 nmol)
- Silencer FAM-标记阴性对照1号 siRNA(货号AM4620, 5 nmol)
如果使用体内级别的Silencer siRNA,可以选:
- Silencer 阴性对照 1号 siRNA(货号4404021)
Silencer Select siRNA阳性对照有:
- Silencer Select GAPDH阳性对照siRNA(货号4390849, 5 nmol; 4390850, 40 nmol; 4404024, 250 nmol)
- Silencer Select MALAT1阳性对照siRNA,靶向非编码siRNA (货号4455877, 5 nmol)
如果使用体内级别的Silencer Select siRNA,可以选:
- Silencer Select GAPDH in vivo(货号44404024)
Silencer Select siRNA阴性对照有:
- Silencer Select阴性对照1号 siRNA (货号4390843, 5 nmol; 4390844, 40 nmol)
- Silencer Select阴性对照2号 siRNA (货号4390846, 5 nmol; 4390847, 40 nmol)
如果使用体内级别的Silencer Select siRNA,可以选:
- Silencer Select阴性对照1号 siRNA, 体内级别 (货号4404020, 250nmol)
Is there a positive control for siRNA which target non-coding RNA? How about a negative control?
We do have a positive control siRNA targeting MALAT-1, which is a ncRNA localized mostly in the nucleus (Cat. No. 4455877). Silencer Select Negative Controls #1 and 2 can be used as the negative controls.
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.
How critical is it to include positive and negative controls in siRNA experiments?
A positive control siRNA such as the Silencer Select GAPDH siRNA will demonstrate efficient transfection and help to optimize transfection conditions. A negative control siRNA is necessary to serve as a baseline for gene expression.
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.