Pierce™ 小鼠 IgG1 Fab 和 F(ab')2 微型制备试剂盒
Pierce™ 小鼠 IgG1 Fab 和 F(ab')2 微型制备试剂盒
Pierce™ 小鼠 IgG1 Fab 和 F(ab')2 微型制备试剂盒
Pierce™ 小鼠 IgG1 Fab 和 F(ab')2 微型制备试剂盒
Pierce™ 小鼠 IgG1 Fab 和 F(ab')2 微型制备试剂盒
Thermo Scientific™

Pierce™ 小鼠 IgG1 Fab 和 F(ab')2 微型制备试剂盒

使用固定化的无花果蛋白酶有效消化小鼠 IgG1 抗体制备 Fab 或 F(ab')2 片断。
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货号数量
4468010份抗体样品,每份含 25 至 250μg IgG
货号 44680
价格(CNY)
8,556.00
Each
添加至购物车
数量:
10份抗体样品,每份含 25 至 250μg IgG
请求批量或定制报价
价格(CNY)
8,556.00
Each
添加至购物车

Thermo Scientific™ Pierce 小鼠 IgG1 Fab 和 F(ab')2 制备试剂盒使用固定化无花果蛋白酶将小鼠 IgG1 高效酶切为 Fab 或 F(ab')2 片段,具体取决于所选的反应条件。

该试剂盒使用无花果蛋白酶对整个 IgG 的 Fc 部分进行酶促酶切,得到 Fab 或 F(ab')2 片段。由于无花果蛋白酶提供为固定化的球珠状琼脂糖树脂形式,消化反应可以通过从 IgG 溶液中去除树脂而轻易地终止,获得不含有酶的消化产品。优化的操作程序和高性能的脱盐作用以及蛋白 A 离心柱使试剂盒易于使用。

特点:

  • 无酶的酶切产品 – 固定化无花果蛋白酶(微珠状琼脂糖树脂)可用于控制酶切反应,并完全去除蛋白酶中的所得抗体片段

  • 针对小鼠 IgG1 进行了优化 – 该试剂盒程序专门针对单克隆小鼠 IgG1 抗体进行了优化,可为该抗体亚类提供可能的较佳得率

  • 高容量 – 该试剂盒可一次使用多达 4mg IgG 进行酶切和纯化。使用标准大小试剂盒可对 0.25 至 4mg IgG 进行片段化,微型试剂盒可对 25 至 250μg IgG 进行片段化

  • 完整且易于使用 – 试剂盒包括制备和纯化抗体片段所需的所有试剂、离心柱和附件

包括:

琼脂糖树脂无花果蛋白酶、半胱氨酸、消化缓冲液、蛋白 A 离心柱(带结合和洗脱缓冲液)、脱盐离心柱及配件

仅供科研使用。不可用于诊断程序。
规格
套装内容Pierce Mouse IgG1 Fab and F(ab')2 Micro Preparation Kit
数量10份抗体样品,每份含 25 至 250μg IgG
产品线Pierce
Unit SizeEach
内容与储存
足够用于:10 个抗体样品、每个样品含有 25 至 250 μg IgG
• Pierce 无花果蛋白酶琼脂糖,0.8 mL
• 半胱氨酸-HCl,1 g
• 小鼠 IgG1 酶切缓冲液,120 mL
• NAb 蛋白 A Plus 离心柱,0.2 mL,2 柱
• 蛋白 A 结合缓冲液,50 mL
• IgG 洗脱缓冲液,50 mL
• Zeba 脱盐离心柱,7K MWCO,0.5 mL,10 柱
• 离心柱,0.8 mL,10 柱
• 微量离心管,2 mL,30 支

在 4–8°C 下储存。

常见问题解答 (FAQ)

Can I reuse the immobilized digestion enzymes in the Thermo Scientific IgG Fragmentation kit?

No. For best results, use the immobilized enzyme once and discard after use.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How long does IgG digestion take using the Thermo Scientific IgG Fragmentation kit?

The digestion time is a function of the IgG concentration and source. For rabbit, mouse and human IgG, optimized digestion times are given in the instructions for each kit. Digestion times listed in the product instructions result in 80-90% digestion, although efficiency varies depending on IgG preparation and source.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

In the Thermo Scientific IgG Fragmentation kit, when digesting an IgG that does not bind to Protein A, how do I purify the Fab and F(abÁ)2 fragments?

If the IgG is a species or subclass that does not bind to Protein A, researchers can immobilize an antibody specific for the desired fragment. For example, to purify digested rat IgG2a, use the Aminolink Plus Immobilization Kit (Cat. No. 44894) to immobilize goat anti-rat Fc antibody. Our Tech Tip #34 and other web resources list IgG binding properties of Protein A. (Because Protein G and Protein A/G bind Fab fragments, they are not effective for this application.)

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I purchase the immobilized enzymes (proteases) from the Thermo Scientific IgG Fragmentation kit separately?

Yes. The three immobilized enzymes are available in 5 mL package sizes as shown below:

• Immobilized Ficin, Cat. No. 44881
• Immobilized Papain, Cat. No. 20341
• Immobilized Pepsin, Cat. No. 20343

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How many Thermo Scientific IgG Fragmentation kits are available and which enzymes are included?

There are six Thermo Scientific IgG fragmentation kits:

• Fab Preparation Kit (Papain), Cat. No. 44985
• Fab Micro Preparation Kit (Papain), Cat. No. 44685
• F(abÁ)2 Preparation Kit (Pepsin), Cat. No. 44988
• F(abÁ)2 Micro Preparation Kit, (Pepsin), Cat. No. 44688
• Mouse IgG1 Fab and F(abÁ)2 Preparation Kit (Ficin), Cat. No. 44980
• Mouse IgG1 Fab and F(abÁ)2 Micro Preparation Kit (Ficin), Cat. No. 44680

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.