QuantStudio™ 12K Flex 实时荧光定量 PCR 系统,96 孔模块,便携式计算机
QuantStudio™ 12K Flex 实时荧光定量 PCR 系统,96 孔模块,便携式计算机
Applied Biosystems™

QuantStudio™ 12K Flex 实时荧光定量 PCR 系统,96 孔模块,便携式计算机

QuantStudio™ 12K Flex 系统是一个高度灵活、全面的实时荧光定量 PCR 平台,可在一台仪器中利用极佳 Applied Biosystems™ 实时技术了解更多信息
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货号数量
44710501 system
货号 4471050
价格(CNY)
-
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数量:
1 system
QuantStudio™ 12K Flex 系统是一个高度灵活、全面的实时荧光定量 PCR 平台,可在一台仪器中利用极佳 Applied Biosystems™ 实时技术。QuantStudio 12K 实时荧光定量 PCR 系统包括易于更换的模块,可容纳 OpenArray™ 板、TaqMan™ 阵列卡、384 孔、96 孔和 FAST 96 孔板,允许您选择适合您项目(无论是现在还是将来)的规格,以便在您的研究中获得较佳灵活性和一致性。

Thermo Fisher Cloud
Thermo Fisher Cloud 为科学家提供了存储、分析和共享数据的安全场所。Thermo Fisher Cloud 中的 Applied Biosystems qPCR 分析模块是基于网络浏览器的软件工具,灵活、快速且易于使用。这些工具与 Mac 或 PC 以及大多数 Applied Biosystems 仪器文件类型兼容。

QuantStudio 12K Flex 系统的直观软件、简单的触摸屏设置、自动化集成、轻松的模块交换以及易于使用的 OpenArray 组件设计用于使您可以立即开始并缩短手动操作时间。为您的未来制定计划,并使用罕见的一体式 qPCR 仪器节省时间、金钱和实验室空间,从而实现较大通量的多种应用。

灵活性 & 可扩展性
包括五个可互换模块:QuantStudio 12K Flex 模块可容纳 96 孔、FAST 96 孔、384 孔、TaqMan 阵列卡和 OpenArray 板,可在一次运行中轻松扩展 1 到 12,000 个数据点。当您深入研究时,只需使用一台仪器即可提高通量。当您开始项目时,从 96 孔规格的实验开始,然后按比例放大至更高通量,以获得您需要的具有统计学意义的答案。此外,您还可以使用 TaqMan 阵列卡和 OpenArray 板等新型规格,使用所选基因组轻松验证和筛选目标靶标。

• 与全范围的 TaqMan 测定兼容: QuantStudio 12K Flex 系统经过优化,可获得所有 TaqMan 测定的清晰干净数据,可用于多种应用,包括基因表达、microRNA 和非编码小 RNA、SNP 基因分型、蛋白分析、Protein Thermal Shift、高分辨率熔解、拷贝数变化和病原体检测。

•提供从 qPCR 到数字 PCR 的无缝转换:使用 QuantStudio 数字 PCR 试剂盒和配备 QuantStudio OpenArray 模块的 DigitalSuite™ 软件将 qPCR 转换为数字 PCR 模式,以提高核酸实验的精密度和灵敏度。

•放心: 周五可提前离开,在家中运行监视器,可了解系统在超过 100 次运行后可以安全存储。

增加通量
增强型 OptiFlex™ 系统: 基于 Applied Biosystems 技术,增强型 OptiFlex 系统为单次运行中收集 12,000 个数据点所需的准确和敏感数据分析提供增强荧光检测。

•空前的覆盖率和样品处理量: OpenArray 规格可加速基因组校验和筛选,每个工作日可驱动超过 150,000 个数据点,从而使大多数项目可以在数天而非在数周内完成。

• 简单的 OpenArray 工作流程: QuantStudio Open Array 板采用箱装形式,有助于使用 Accufill™ 系统装载样品时实现轻松处理并确保较佳数据质量。将四个阵列轻松加载到 QuantStudio 12K Flex 仪器中,以实现超过 12,000 个数据点。集成式样品追踪软件可帮助您轻松追踪整个项目过程中的样品。

• 集成式分析和质量系统:新的直观触摸屏界面和集成日历功能可安排仪器的使用时间。提供其他分析软件,如 DigitalSuite v1.0、ExpressionSuite v1.0 和 Genotyper v1.1,可帮助您分析数百个靶标和样品,并获得所需的统计效力。

QuantStudio 12K Flex 软件
• 为新手和有经验的用户提供直观界面和创新设计
• 便利的无人值守操作
• 改进的不同模块类型数据分析
• 完全兼容高通量环境

可扩展的软件架构
提供可选的加载项来升级现有软件:
符合 21 CFR 第 11 部分要求:此可选 SAE 模块有助于记录的安全性、稽查和电子签名,以实现完整数据的可追溯性。

• 高分辨率熔解 (HRM):使用内置的 MeltDoctor™ 方案和校准,您可以花费更少时间优化运行以及使用更多时间自定义您的结果。通过快速更改分析设置定义要查看的变体数量。

• DigitalSuite 软件 v1.0: 用于需要灵敏检测罕见靶标以及需要精确测定靶标丰度微小差异的 OpenArray 模块用户。

• ExpressionSuite 软件 v1.0: 独立基因表达软件配备增强型分析工具,可分析超过200个384孔板或10个 OpenArray 板。

了解更多有关可用融资方案和计划的信息›

仅供科研使用。不可用于诊断程序。
规格
显示类型触摸屏
适用于(设备)QuantStudio™
产品规格单管
产品线QuantStudio
数量1 system
运输条件室温
模块规格可互换
Unit SizeEach
内容与储存
96孔模块,便携式计算机

常见问题解答 (FAQ)

What is the difference between continuous melt curves and step and hold melt curves on the Applied Biosystems Real-Time PCR Instruments?

Continuous melt curve: increases the temperature by the ramp increment (degrees C/sec) so that the temperature will change at a constant rate set in the protocol.
Step and Hold melt curve: increases the temperature by the ramp increment (degrees C) and then holds at that temperature for the time specified by the user.

The amount of data collected will depend on the filter(s) selected. The more filters you use, the longer the acquisition will take and the fewer data points will be collected.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

I recently switched the block in my real-time PCR instrument. Now it shows the status as 'not calibrated' for all the calibration plates, even though my calibrations are current. What is causing this issue?

The issue might be due to the block not being properly seated in the instrument. Please follow the steps below:

  1. Remove the block from the instrument.
  2. Reinsert the block into the instrument.


If the problem persists after reseating the block, please contact our Technical Support team for further assistance.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Can I import a standard curve for my real-time PCR data into the Design & Analysis Software (v2.x)?

It is possible to import a standard curve into the Design & Analysis Software (v2.x) if you are analyzing results from one of the following Applied Biosystems real-time PCR instruments:
• 7900HT Fast Real-Time PCR System
• 7500/7500 Fast Real-Time PCR System
• StepOne/StepOne Plus Real-Time PCR System
• ViiA 7 Real Time PCR System
• QuantStudio 1 Real-Time PCR System
• QuantStudio 3 & 5 Real-Time PCR System
• QuantStudio 6 & 7 Flex Real-Time PCR System
• QuantStudio 6 & 7 Pro Real-Time PCR Systems
• QuantStudio 12K Flex Real-Time PCR system

Follow the instructions below to import a standard curve for analysis in the Design & Analysis Software (v2.x):
1. Open the data file in the Design & Analysis Software (v2.x).
2. Click on Actions.
3. Click on "Standard Curve Analysis Setting".
4. Select "External Standard Curves" and click on "Import".
5. Browse to the proper .csv file and click "Open".
6. Click "Apply".

Can I import a standard curve for my real-time PCR data into the QuantStudio 12K Flex Software?

If you are analyzing results from the QuantStudio 12K Flex Real-Time PCR System, it is possible to import a standard curve into the QuantStudio 12K Flex Software following the instructions below:
1. Open the data file in the QuantStudio 12K Flex Software.
2. Go to Analysis Settings.
3. Click on the "Standard Curve Settings" tab.
4. Select the option to "Use a standard curve imported from another experiment" and click on "Import".
5. Browse to and highlight the proper data file and click "Select".
6. Click Apply Analysis Settings.

What is the difference between a Fast 96-well and standard 96-well block?

There are two main differences between a Fast 96-well and standard 96-well block for the Applied Biosystems real-time PCR systems:
• Reaction well volume: The Fast 96-well blocks have 0.1 mL reaction wells and are compatible with 0.1 mL plates and tubes. The standard 96-well blocks have 0.2 mL reaction wells and are compatible with 0.2 mL plates and tubes.
• Ramp rate: The fast 96-well blocks have a higher maximum ramp rate than standard blocks for the same real-time PCR system. However, both the fast 96-well and standard 96-well blocks are capable of running standard and fast chemistry.

Note: For newer instruments (QuantStudio 3 and 5 Real-Time PCR Systems and later) "0.1 mL" and "0.2 mL" are being used in place of "fast" and "standard" as designations for the two 96-well block formats.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.