Can I add files (.ab1) from a CD to Sequencing Analysis software?
The .ab1 files from a CD are locked (read-only), so you won't be able to analyze them. To work with files from a CD:
1. Drag the .ab1 files from the CD to the hard drive (not the desktop).
2. Select the files, right-click and select Properties.
3. De-select the Read Only check box and click OK.
4. The .ab1 files can now be added to Sequencing Analysis Software and analyzed.
Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Software Support Center.
In the Sequencing Analysis software, I see yellow color in the BC check box in the Sample Manager; what does it mean?
In the Sequencing Analysis software, after Analyzing your samples, the BC check box will change color, reflecting the status of the analysis.
Green: Analysis was successful.
Yellow: Poor quality data (if using KB Basecaller, a partial output file is available).
Red: Analysis Failed
The PP and P check boxes will only contain Green or Red status colors.
Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Software Support Center.
In the Sequencing Analysis software, what is the difference between using the "Show" button in the tool bar and clicking boxes in the "Show" column and when should I use them?
In the Sequencing Analysis software, in order to display the data, the check box in the Show column needs to be checked next to the samples you wish to display. The "Show" button was designed to automatically check the show boxes for selected samples. You can select the samples by clicking on the row number. Holding down the shift key while clicking on the row number will allow you to select continuous samples, holding the control key while clicking the row number will allow you to select individual samples.
Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Software Support Center.
In the Sequencing Analysis software, can I alter the bases in my sequences?
Yes, bases can be edited in the Sequencing Analysis software. You can insert or delete bases in your electropherogram by simply typing in the change or highlighting the base and pressing the delete key. After making the changes, you must save the sample file in order for those changes to be reflected in your .seq files. Re-analyzing the samples after making any changes will remove the changes made to the sample file.
Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Software Support Center.
In the Sequencing Analysis software, can I change the size of the Windows displayed when multiple files are displayed?
In the Sequencing Analysis software, you can zoom in and out of the data or choose to view the full view of the samples by going to the View menu and selecting one of the display options or by clicking on the appropriate display button. When you zoom in or out, all samples that are being displayed at that time will be affected.
Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Software Support Center.
