Ion Torrent™

Ion Xpress™ RNA-Seq 条形码 1-16 试剂盒

The new Ion Xpress™ RNA-Seq Barcode 1-16 Kit provides a set of 16 unique barcode primers for sequencing RNA on the Ion Torrent PGM™ semiconductor sequencer.

货号	数量
4475485	160 Reactions

货号 4475485

价格（CNY)

160 Reactions

新的 Ion Xpress™RNA-Seq 条形码 1-16 试剂盒提供了一组 16 种独特的条形码引物，用于在 Ion Torrent PGM™ 半导体测序仪上进行 RNA 测序。当与 Ion Total RNA-Seq 试剂盒 v2 配合使用时，Ion Xpress™ RNA-Seq 条形码 1-16 试剂盒可让用户在模板制备前混合多达 16 个片段文库，然后进行多通路测序分析，从而简化小型和全转录组 RNA 的 Ion 半导体测序工作流程。

特点和优势：
•高效的多通路技术 - 可在单个测序芯片上实现多达 16 个片段文库的多通路检测
• 序列和流动循环经过优化，可在池中平均显示所有条形码
• 多通路降低了模板制备和测序的成本和处理要求
• 通过设计内置了稳健的纠错功能，增加了样品鉴定的可信度

使用条形码引物的高效多通路技术可实现更高通量样品测序
使用带条形码的文库的多通路技术使得每次运行可检测多达 16 份样品，性价比更高，从而显著降低了模板制备和测序的成本和处理要求。

为提高性能和效率对序列和流动循环进行了优化
条形码引物包含的序列经过优化，以在池中平均显示所有条形码，可以最少流动循环进行检测，并且仅需 20 个碱基即可进行条形码和接头测序，从而实现更经济的多通路测序运行。

通过设计内置了稳健的纠错功能
内置纠错功能可显著减少样品分配错误的可能性。

Ion Xpress™ RNA-Seq 条形码 01-16 试剂盒设计用于为每个条形码创建 10 个多通路文库，或共创建 160 个多通路文库。

仅供科研使用。不得用于任何动物或人类的治疗或诊断。

仅供科研使用。不可用于诊断程序。

适用于（设备）Ion PGM™ 系统

反应次数160

数量160 Reactions

运输条件干冰

工作流程步骤文库条形码

Unit SizeEach

内容与储存

16 管，Ion Xpress™ RNA BC 01 - BC 16 各 12 µL - 在 20°C 下储存

1 管，192 µL Ion Xpress™ RNA 3' 条形码引物 - 在 20°C 下储存

常见问题解答 (FAQ)

Where can I find the sequences for the Ion Xpress RNA-Seq Barcodes 1-16 (Cat. No. 4475485)?

The Ion Xpress RNA-Seq Barcode 1-16 sequences are the same as the DNA library Ion Xpress Barcode Adapter 1-16 sequences and can be found under References in the Torrent Browser.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Why does the Ion Total RNA-Seq Kit v2 (Cat. No. 4475936) specifically require the Ion Xpress RNA-Seq Barcodes 1-16 Kit (Cat. No. 4475485)? Why can't I use the DNA barcodes provided in the Ion Xpress Barcode Adaptors 1-96 Kit (Cat. No. 4474517)?

The Ion Total RNA-Seq Kit v2 workflow uses a different method for adding the barcode sequences to the library than the standard Ion DNA libraries. For standard DNA library construction, the barcoded adapters are directly ligated to the DNA inserts. For Ion Total RNA-Seq libraries, the barcode adapter sequence is added during the final library amplification.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many samples can I multiplex on a single chip for sequencing on the Ion PGM or Proton System?

The number of samples that can be multiplexed in a single sequencing run depends on the capacity of the chip, the size of the library, and the required coverage. A table of approximate capacities based on the size of the library and chip can be found in the Ion Ampliseq Preparation User Guide (https://tools.thermofisher.com/content/sfs/manuals/MAN0006735_AmpliSeq_DNA_RNA_LibPrep_UG.pdf) within the section “Strategies for combining Ion Ampliseq libraries.”

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.