How does the quantification solution provided by the Quantifiler Trio and HP DNA Quantification Kits and HID Real-Time PCR Software easily integrate with robotics for an automated workflow?
Robotic integration is enabled by the automation-friendly bundling of the Quantifiler Trio and HP DNA Quantification kits combined with the HID Real-Time PCR Analysis Software, which has been designed to facilitate automation with commonly used robotic platforms. In addition, our Human Identification Professional Services (HPS) team has extensive expertise in developing customized robotic scripts and conducting on-site verification and validation testing to help you achieve optimal performance with a wide range of platforms. The HPS team has already developed scripts for the Quantifiler Trio and HP DNA Quantification kits on several of the most common robotic platforms, and would be happy to consult with your lab on how to efficiently implement these solutions into your workflow. Please contact your local human identification (HID) account manager to learn more.
Can I use the basic SDS v1.2.3 software to analyze data generated with the Quantifiler Trio and HP DNA Quantification kits?
We do not recommend using the basic SDS v1.2.3 software. The Quantifiler Trio and HP DNA Quantification kits were developed and validated using the HID Real-Time PCR Software. The software ensures the optimal performance of new Quantifiler Trio and HP DNA Quantification kit-specific functionality, such as the automatic calculation of M:F ratios, Degradation Index and streamlined setup for the latest STR analysis kits including the Identifiler Plus, NGM Select and GlobalFiler kits.
What instrument can I use with the Quantifiler Trio and HP DNA Quantification kits and HID Real-Time PCR Software quantification solution?
The Quantifiler Trio and HP DNA Quantification kits and HID Real-Time PCR Analysis Software v1.3 have been validated to perform on the Applied Biosystems 7500 Real-Time PCR System.*
HID Real-Time PCR Analysis Software v1.4 has been validated to perform on the Applied Biosystems QuantSudio 5 and 7500 Real-Time PCR Systems.
* When used for purposes other than human identification, the instruments cited are for Research Use Only. Not for use in diagnostic procedures.
Does the Quantifiler Trio DNA Quantification Kit assay perform well with male:female mixture samples?
We evaluated male:female DNA ratios up to 1:4,000 with 20 pg/µL male DNA and were able to accurately quantify the male and female DNA fractions. For more detailed information, you can refer to the Mixture Study in the Quantifiler HP and Trio DNA Quantification Kits user guide.
How is female DNA detected with the Quantifiler Trio and HP DNA Quantification kits? Is there a specific marker for females?
By subtracting the male DNA concentration from the human DNA concentration, the concentration of female DNA can be approximated.