大信息量工作流工具 Quantifiler Trio DNA 定量试剂盒已作为完整的 DNA 检测系统的一部分经过优化和验证,该检测系统含有 AmpFLSTR PCR 扩增试剂盒。化学方法与常用的提取技术兼容且易整合入现代实验室工作流程。这使得 Quantifiler Trio 试剂盒成为改进和整合工作流程的关键组分,从而更有效地处理法医个案检材。该试剂盒和软件均可与大多数液体处理自动化平台配合进行自动化工作。
与 Quantifiler Duo 试剂盒对比 Quantifiler Trio DNA 定量试剂盒和 Quantifiler Duo DNA 定量试剂盒有几个具体的区别,包括: 靶基因座:Quantifiler Trio 试剂盒采用多拷贝靶基因座以改进检测灵敏度。有三个人类特异性靶基因座:小常染色体、大常染色体和 Y 染色体靶点。每一个都由分散在不同的常染色体上的多拷贝组成(小常染色体和大常染色体)或 Y 染色体上的多拷贝。 优化化学方法:该检测的化学过程已经过优化,具有更高效的多通路技术、更快的 PCR 循环时间(∼1 小时)以及更好的抑制剂耐受性。整体性能改进使 Quantifiler Trio 试剂盒更好地匹配新型 STR 试剂盒增强的性能,后者设计用于容纳更具挑战性的样品,如 Identifiler Plus、NGM SElect 或 GlobalFiler PCR 扩增试剂盒。 DNA 标准品:Quantifiler Trio DNA 标准品是一种混合的人基因组小分子量 DNA,可提高稳定性、均一性并与法医学样品相关。有一个五点标准曲线方案,通过在平板上增加多达 6% 的样品帮助提高效率。 包装特征:为帮助实验室控制和尽可能减少试剂污染的可能性,以及更好地适应自动化平台,Quantifiler Trio DNA 定量试剂盒有一些关键的包装特征: --四个可用于 100 次反应的单独使用的螺帽管(1 个管将处理一个 96 孔板) --DNA 标准品包装在单独的盒中。 --试剂和 DNA 标准品盒均装在带有可剥离和粘贴的试剂批次条码标签的 STR 型套筒中运输。这使客户能够在到达实验室后轻松分出试剂,以便进行特殊储存。
规格
产品线Quantifiler
数量400 reactions
靶标Large autosomal (LA) human target,Y chromosome,small autosomal (SA) human target
Unit SizeEach
内容与储存
收货后在 -5°C 至 -30°C 下储存,初次使用后在 2°C 至 8°C 下储存。
常见问题解答 (FAQ)
How does the quantification solution provided by the Quantifiler Trio and HP DNA Quantification Kits and HID Real-Time PCR Software easily integrate with robotics for an automated workflow?
Robotic integration is enabled by the automation-friendly bundling of the Quantifiler Trio and HP DNA Quantification kits combined with the HID Real-Time PCR Analysis Software, which has been designed to facilitate automation with commonly used robotic platforms. In addition, our Human Identification Professional Services (HPS) team has extensive expertise in developing customized robotic scripts and conducting on-site verification and validation testing to help you achieve optimal performance with a wide range of platforms. The HPS team has already developed scripts for the Quantifiler Trio and HP DNA Quantification kits on several of the most common robotic platforms, and would be happy to consult with your lab on how to efficiently implement these solutions into your workflow. Please contact your local human identification (HID) account manager to learn more.
Can I use the basic SDS v1.2.3 software to analyze data generated with the Quantifiler Trio and HP DNA Quantification kits?
We do not recommend using the basic SDS v1.2.3 software. The Quantifiler Trio and HP DNA Quantification kits were developed and validated using the HID Real-Time PCR Software. The software ensures the optimal performance of new Quantifiler Trio and HP DNA Quantification kit-specific functionality, such as the automatic calculation of M:F ratios, Degradation Index and streamlined setup for the latest STR analysis kits including the Identifiler Plus, NGM Select and GlobalFiler kits.
What instrument can I use with the Quantifiler Trio and HP DNA Quantification kits and HID Real-Time PCR Software quantification solution?
The Quantifiler Trio and HP DNA Quantification kits and HID Real-Time PCR Analysis Software v1.3 have been validated to perform on the Applied Biosystems 7500 Real-Time PCR System.*
HID Real-Time PCR Analysis Software v1.4 has been validated to perform on the Applied Biosystems QuantSudio 5 and 7500 Real-Time PCR Systems.
* When used for purposes other than human identification, the instruments cited are for Research Use Only. Not for use in diagnostic procedures.
Does the Quantifiler Trio DNA Quantification Kit assay perform well with male:female mixture samples?
We evaluated male:female DNA ratios up to 1:4,000 with 20 pg/µL male DNA and were able to accurately quantify the male and female DNA fractions. For more detailed information, you can refer to the Mixture Study in the Quantifiler HP and Trio DNA Quantification Kits user guide.
How is female DNA detected with the Quantifiler Trio and HP DNA Quantification kits? Is there a specific marker for females?
By subtracting the male DNA concentration from the human DNA concentration, the concentration of female DNA can be approximated.