Thermo Scientific Slide-A-Lyzer 迷你透析杯 (10K MWCO) 是配有集成的低结合膜的一次性聚丙烯杯，用于透析及从 10 µL 至 2 mL 体积高效回收 >10 kDa 的蛋白和大分子。

Slide-A-Lyzer 迷你透析杯（10K MWCO，0.1 mL）的特点：

•三种便利规格– 0.1、0.5 和 2 mL 样品的透析杯分别适合标准的 1.5 mL 微量离心管、15 mL 和 50 mL 锥形管
•出色的样品回收率– 与过滤和树脂系统相比，低结合塑料材质和较小的膜表面积可将样品损失尽可能降低
•一步式方案– 使用移液器将样品吸取到迷你透析杯中并将其置于含透析缓冲液的管中；无需费力的组装、装置准备或昂贵的设备
•100% 经过防漏检测– 创新的设计不会导致出现在自制设备中出现的“毛细虹吸”现象
•节省时间– 进行核酸和蛋白脱盐（回收率 >95%）的时间少于 15 分钟，这提高了样品的分离度、定量和偶联性

这些 10K MWCO 透析杯有三种规格（0.1、0.5 和 2 mL 容量），其设计可使用标准实验室移液器方便地进行样品添加和去除。迷你透析杯可有效便利地在 4 至 8 小时内完成典型样品的脱盐或缓冲液置换。与其他小样品分离设备不同，其不需要组装设备，不需要注射器适配器，也不需要费力的步骤来处理小体积样品。该再生纤维素膜与多种常见化学品和缓冲液兼容。

Slide-A-Lyzer 迷你透析杯可用作透析小容积核酸和蛋白质样品而无过度样品损失的便捷方法。使用这些装置，可完成低分子量污染物去除、缓冲液更换、脱盐、平衡透析、微型-偶联反应和浓缩。Slide-A-Lyzer 迷你透析杯在 HEPA 过滤器/清洁环境中生产，并在组装后自动包装以确保它们不含污染物并可随时使用。

0.5 mL 和 2 mL 迷你透析杯可分别插入 15 mL 和 50 mL 锥形管并盖上盖子。该离心管可以稳固地放置于标准实验室支架或摇床中；该特点节省了空间，较大限度地减少了泄漏或污染的风险并且无需浮漂或者标有大烧杯缓冲液。使用这些迷你透析杯可在 4 至 8 小时内完成低分子量污染物的去除、缓冲液的置换和脱盐，其效率、比率和回收率与采用大透析液量的常规透析非常相似（查看数据）。

0.1 mL 的迷你透析杯设计为可以盖合（含盖）并可以插入标准的 1.5 mL 微量离心管。或者，可以在 Slide-A-Lyzer 迷你透析杯浮子中放置多达 25 个迷你透析杯，以便在一个大烧杯中进行多个透析。使用 0.1 mL 迷你透析杯，可在 10 分钟内使用 1 升 pH 值 9.4 的碳酸氢盐缓冲液平衡处理 100 µL pH 值 2.8 的甘氨酸缓冲液。

与所有透析应用一样，使用这类透析杯的每份样品观察到的透析率取决于多种因素，包括膜-样品体积比、温度和搅拌速率；以及穿过膜的分子的分子量、形状、水合度和电荷以及所使用缓冲液的 pH 值、离子强度和溶剂特性。

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