GlycoLink™ 偶联催化剂

Thermo Scientific GlycoLink 偶联催化剂可缩短反应时间和提高醛-酰肼偶联效率，在 4 小时内实现超过 90% 的糖蛋白偶联。GlycoLink 偶联催化剂的特点：•经优化—可催化醛-酰肼和醛-烷氧基胺偶联反应•高效—尽可能提高偶联效率，缩短所需的偶联反应孵育时间•温和—不会干扰蛋白功能，通过脱盐或透析可轻松去除GlycoLink了解更多信息

货号	数量
88944	100 mL

货号 88944

价格（CNY)

934.00

添加至购物车

100 mL

请求批量或定制报价

价格（CNY)

934.00

添加至购物车

Thermo Scientific GlycoLink 偶联催化剂可缩短反应时间和提高醛-酰肼偶联效率，在 4 小时内实现超过 90% 的糖蛋白偶联。

GlycoLink 偶联催化剂的特点：

•经优化—可催化醛-酰肼和醛-烷氧基胺偶联反应
•高效—尽可能提高偶联效率，缩短所需的偶联反应孵育时间
•温和—不会干扰蛋白功能，通过脱盐或透析可轻松去除

GlycoLink 偶联催化剂是由苯胺和醋酸钠缓冲液组成的两组分试剂盒。该催化剂加速了酰肼和烷氧基胺部分与反应性醛的偶联速率，后者是由糖蛋白中顺式二醇的高碘酸盐氧化生成的。GlycoLink 偶联催化剂可提高这些反应的偶联效率，降低偶联反应所需的标记试剂的摩尔量过量。该催化剂还可用于提高氧化糖蛋白或其他含碳水化合物分子与酰肼树脂的结合效率。

应用
•用烷氧基胺-生物素标记含醛分子
•用酰肼-生物素标记含醛分子
•提高含醛分子与酰肼树脂的偶联效率

通过糖蛋白中顺式二醇的偏高碘酸钠氧化生成反应性醛是一种适用于多种蛋白质标记和检测方法的实用技术。GlycoLink 偶联催化剂（苯胺）充当亲核催化剂来加速通过席夫碱中间体实现的键形成，从而提高了这些反应的效率。该催化剂还可降低标记反应中所需的酰肼或烷氧基胺量（摩尔量过量），因此无需使用 DMSO 溶解高浓度的标记试剂。

更多产品数据
•改善糖蛋白固定和偶联

仅供科研使用。不可用于诊断程序。

产品线GlycoLink™

数量100 mL

产品类型偶联催化剂

Unit SizeEach

内容与储存

储存于 4°C。

常见问题解答 (FAQ)

What is the difference between GlycoLink and CarboxyLink Gels?

GlycoLink gel is hydrazide-activated crosslinked beaded agarose, and it is useful for coupling glycoproteins via aldehydes formed from their sugars by sodium meta-periodate oxidation. Reaction of aldehydes with hydrazide- activated resin is catalyzed by aniline resulting in >90% coupling in 4 hours or less. CarboxyLink gel on the other hand is crosslinked beaded agarose activated with diaminodipropylamine (DADPA) and is useful for immobilizing carboxyl- containing biomolecules after EDC activation. Actually, both resins can be used with EDC to couple ligands via carboxylic acids.

Note: Both immobilization chemistries are available on UltraLink Support as UltraLink Hydrazide and UltraLink DADPA respectively.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Will Glycolink Gel work with chicken IgY?

Yes, Chicken IgY can be immobilized using GlycoLink Gel, due to the high level of glycosylation of the IgY.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Will GlycoLink Gel work well for conjugating all types of antibodies?

No. Monoclonal antibodies are typically under-glycosylated, and coupling efficiency to CarboLink Gel may be low. However, many monoclonal antibodies can be immobilized using GlycoLink Gel. Try the Pierce Glycoprotein Carbohydrate Estimation Kit (Cat. No. 23260) to determine if your monoclonal antibody is glycosylated.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the benefits of using a GlycoLink Coupling Resin?

The benefits include high-capacity immobilization of 1-10 mg of oxidized antibody or other glycoprotein per milliliter of resin. Immobilization is fast (in as few as 30 mins) and can achieve at least 90% coupling of most glycoproteins in less than 4 hours. The hydrazide-activated UltraLink resin conjugates only to purified glycoproteins whose sugar groups have been gently oxidized with periodate.

No stabilization step is required when using this resin, and antibody function is preserved as IgGs are immobilized via the Fc region, keeping both antigen binding sites available for capturing targets.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.