足够用于:从 10 份分别高达 640 μL(1 至 1.5 mg 总蛋白)的样品中分离对 WGA 具有强亲和力的糖蛋白 • WGA 外源凝集素树脂,1.1 mL • 结合/洗涤缓冲液 (5X),6.5 mL • 洗脱缓冲液,5 mL • 离心柱,10 个柱 • 收集管,20 个管
在 4°C 下储存。
常见问题解答 (FAQ)
What is the difference between your Pierce Glycoprotein Isolation Kit, ConA and WGA?
The ConA kit (Cat. No. 89804), which has concanavalin A immobilized on the resin, recognizes alpha-linked mannose, and to a lesser extent, terminal glucose residues. The WGA kit (Cat. No. 89805), which has wheat germ agglutinin immobilized on the resin, recognizes N-acetyl glucosamine (GlcNAc) groups and sialic acid.
Can I isolate my proteins based on their post-translational modification (PTM) or active binding site?
Enrichment of specific proteins or protein complexes can most easily be accomplished by using target-specific immobilized antibody or PTM specific affinity binding. We offer several kits targeting phosphorylated proteins, alpha- linked mannose and terminal glucose residues, N-acetyl glucosamine, polyubiquinated proteins, ATP binding sites, and active serine hydrolase enzymes. Choose the right protein enrichment kit for your application using the following link (http://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-purification-isolation/protein-purification/protein-enrichment.html).