Zeba™ 脱盐离心柱、板和纯化柱,7K MWCO,0.5–100 mL
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Zeba™ 脱盐离心柱、板和纯化柱,7K MWCO,0.5–100 mL
Thermo Scientific™

Zeba™ 脱盐离心柱、板和纯化柱,7K MWCO,0.5–100 mL

使用即用型 Thermo Scientific Pierce Zeba 脱盐色谱柱、板和纯化柱实施高通量蛋白回收 (7K MWCO) 和样品脱盐,以进行质谱、HPLC、毛细管电泳、代谢物筛选和测定方法开发。
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货号数量产品规格容积(公制)
899345 columns x 1 mLChromatography Cartridge1 mL
8987725 Columns15 mL 锥形离心柱75 μL
8987850 Columns15 mL 锥形离心柱75 μL
A400024695 Columns15 mL 锥形离心柱20 mL
A4000247510 Columns15 mL 锥形离心柱20 mL
8989025 Columns15 mL 锥形离心柱2 mL
898915 Columns15 mL 锥形离心柱5 mL
8989225 Columns15 mL 锥形离心柱5 mL
898935 Columns15 mL 锥形离心柱10 mL
8989425 Columns15 mL 锥形离心柱10 mL
898895 Columns15 mL 锥形离心柱2 mL
8988225 Columns15 mL 锥形离心柱0.5 mL
8988350 Columns15 mL 锥形离心柱0.5 mL
899351 column x 5 mLChromatography Cartridge5 mL
898072 Plates96 孔过滤板96-well
898084 Plates96 孔过滤板96-well
货号 89934
价格(CNY)
3,601.00
Each
添加至购物车
数量:
5 columns x 1 mL
产品规格:
Chromatography Cartridge
容积(公制):
1 mL
请求批量或定制报价
价格(CNY)
3,601.00
Each
添加至购物车
Perform high-throughput protein recovery and sample desalting for mass spectrometry, HPLC, capillary electrophoresis, metabolite screening, and assay development with ready-to-use Pierce Zeba desalting chromatography columns, plates, and cartridges. Rapidly desalt protein and biological samples for manual or automated liquid chromatography (LC) with ≥95% retention of salts and other small molecular contaminants (<1,000 Da).

These Zeba spin desalting products contain a proprietary high-performance size-exclusion chromatography resin that enables excellent protein desalting and recovery of 7K molecular weight cutoff (MWCO) molecules using a centrifuge format. This resin is ideal for removing low molecular weight compounds including salts, fluorescent dyes, biotin, and other small labelling reagents.

Benefits of Zeba desalting columns, plates, and cartridges include:

  • High recovery—low-binding resin maximizes protein recovery,
  • Fast—no fraction screening or waiting for protein to emerge by gravity flow
  • Economical—great performance at lower cost than other commercially available cartridges
  • Easy-to-use—no cumbersome column preparation or equilibration
  • Flexible—available in spin columns, filter spin plates, and cartridges for a range of needs 

Zeba Spin Desalting Columns 

Convenient, ready-to-use polypropylene columns are the ideal solution for quick and straightforward desalting and buffer exchange of protein samples in a centrifuge format. The pre-dispensed spin columns are offered in various resin fills (75 μL, 0.5 mL, 2 mL, 5 mL, 10 mL, 20 mL). These options make it simple to handle sample volumes between 2 μL and 10 mL.

Zeba Desalting Plates

Provide trouble-free high-throughput desalting and buffer exchange for sample volumes ranging from 20 to 100 μL. Pre-dispensed 96-well filter plates require no resin dispensing or hydration and provide the same high protein recovery as spin columns with at least 95% removal of salts and other small molecules. One plate can process 96 small sample volumes (20 to 100 μL) in five minutes. Compatible with centrifugation for manual or automated purification.

Zeba Desalting Chromatography Cartridges

Compatible with automated liquid chromatography systems and with syringe processing, Zeba desalting cartridges provide fast, easy, and reproducible chromatographic separations and can be regenerated for multiple uses.

仅供科研使用。不可用于诊断程序。
规格
产品类型Desalting Chromatography Cartridge
纯化目标Buffer Exchange, Protein
数量5 columns x 1 mL
容积(公制)1 mL
色谱柱类型Size-exclusion, Proprietary Resin
产品规格Chromatography Cartridge
截留分子量 (MWCO)7.0 kDa
产品线Zeba™
样品体积(公制)0.05–0.25 mL
Unit SizeEach
内容与储存
Upon receipt store at 4–8°C.

常见问题解答 (FAQ)

Besides precipitation with ammonium sulfate, what are some ways I can concentrate my protein sample?

You may remove excess solvent and smaller moieties by centrifugation through a microconcentrator. This will concentrate your protein sample.

(1) Choose microconcentrator tube (available from a variety of commercial suppliers) with a protein cutoff smaller than the molecular weight of the protein in the sample. Check our Pierce Protein Concentrators PES.

(2) Add 1 µL of 20% w/v SDS to a dry microconcentrator tube (if sample does not already contain SDS).

(3) Slowly add sample (a few microliters at a time) to membrane until membrane is completely wet. Centrifuge to near (but not complete) dryness.

(4) If intention is to desalt sample or buffer exchange: Add ~50 µL water to microconcentrator and spin until nearly dry. Repeat buffer exchange. Sample will remain on membrane. Check our Zeba desalting proteins.

(5) Using a new collection tube, invert membrane and spin at low speed (1000 x g) to elute protein from membrane. Add 2X SDS-Sample Buffer containing 10 mM DTT to membrane: vortex, invert and spin. Final volume should be ~20 µL.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

Can Zeba Desalting columns, plates, and cartridges be autoclaved?

Yes, Zeba Desalting columns, plates, and cartridges can be autoclaved at 115 degrees C for 30 min.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

What reagents and conditions are Zeba Desalting columns, plates, and cartridges compatible with?

Zeba Desalting columns, plates, and cartridges are compatible with a range of reagents and conditions. They have been confirmed to be compatible with the following:
- pH range of 3-10
- Mild oxidants/reductants
- Chaotropes (stable in 8 M urea and 6 M guanidine HCl)
- Salts
- Alcohols up to 20%
- Organic solvents such as dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) (we recommend using a step-gradient of increasing concentration of the organic solvent during column equilibration)

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

Can Zeba Desalting Chromatography Cartridges be regenerated and stored after use?

Yes, Zeba Desalting Chromatography Cartridges can be regenerated and stored for later use. The catridges may be reused multiple times without significant loss of performance.

For regeneration, wash the resin with 3-5 column volumes of water or buffer before applying the next sample. For storage, wash the resin with 5 column volumes of 0.02-0.05% sodium azide in water. Attach the supplied bottom cap and then the top plug and store the cartridge at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

How should I store Zeba Spin Desalting Columns, Plates, and Cartridges?

We recommend storing Zeba Spin Desalting Columns, Plates, and Cartridges at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.