What are the estimated Poly-A RNA Control final concentrations when processing expression arrays?
Here are the Poly-A RNA Controls final concentrations (in samples):
- lys 1:100,000
- phe 1:50,000
- thr 1:25,000
- dap 1:7,500
What is the shelf life of the Poly-A RNA Control's first dilution during processing of expression microarrays?
The first dilution of the Poly-A RNA Control can be stored at -20 degrees C for 6 weeks (with up to 4 freeze/thaws).
Can I purchase one, or all, of the four Poly-A Controls separately?
These four controls are only available as a pre-mixed cocktail with the defined concentrations and relative ratios. This combination of concentrations has been tested to provide qualitative information on both sensitivity and dynamic range of the labeling assay. Providing these controls in a pre-mixed format increases ease-of-use and reduces the number of manual dilution steps.
Information on ordering individual poly-A control clones and preparing poly-A stocks is available in Section 2, Chapter 2, and Section 3, Chapter 2, of the GeneChip Expression Analysis Technical Manual.
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
Can I purchase the Poly-A Control Stock and the Poly-A Control Dil Buffer separately?
The Control Stock and the Dilution Buffer are packaged in the same Kit to provide convenience to customers. Currently they cannot be purchased as separate products.
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Do you have any specific recommendation on the type of non-stick tubes to use for diluting the Poly-A RNA Control Stock?
The following tubes were used in-house during product development of this Poly-A RNA Control Kit, and we have not tested any other tubes at this time:
Non-stick RNase-free 0.5 mL microfuge tubes, Ambion, Cat. No. 12350
Non-stick RNase-free 1.5 mL microfuge tubes, Ambion, Cat. No. 12450
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
