GeneChip™ 3' IVT PLUS 试剂盒
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Applied Biosystems™

GeneChip™ 3' IVT PLUS 试剂盒

GeneChip™ 3' IVT PLUS 试剂盒特点:• 低 RNA 输入要求–低至 50 ng了解更多信息
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货号数量
90241630 Reactions
90241510 Reactions
货号 902416
价格(CNY)
-
数量:
30 Reactions
GeneChip™ 3' IVT PLUS 试剂盒特点:
• 低 RNA 输入要求–低至 50 ng 的总 RNA 输入量可用于一轮扩增,让更多样品用于未来的研究
• 表达检测试剂盒–GeneChip 3' IVT 表达试剂盒(3' IVT 表达试剂盒)为了持续生成高质量的数据,而不会影响旧的数据
• 方案优化,无需初始引物退火或 cDNA 纯化步骤即可简化实验流程
• 单日方案,适当输入总 RNA,可快速处理样品
• 磁珠纯化以提高回收率,生成足够的靶向物
• 内置对照,以有效避免故障
提高性能,减少所需试剂,实验方案简单且靶向分子制备较为轻松

3'IVT PLUS 试剂盒包括:
• 所有试剂均需生成并标记 aRNA 靶标
• 即用型磁珠纯化以增强回收率
• Poly -A RNA 对照品
• Hyb 对照品
• 试管组织器,用于快速检测设置

关于 Poly A 对照品
Poly-A 对照品(lys、phe、thr 和 dap)从开始至结束监测靶标标记过程。它们可作为靶标制备和标记效率的敏感性指标。该试剂盒包含四种外源性、预混合的聚腺苷酸化原核对照,可在靶标标记前直接加入 RNA 样品中。其在 Applied Biosystems GeneChip 阵列上产生的信号强度可作为标记反应效率的指标。

关于 Hyb 对照品
20X 真核细胞杂交对照品由片段化的生物 B、生物 C、生物 D 和生物素化混合组成,cre 交错浓度,可直接加入杂交混合物中。这些对照品有助于监测杂交过程,以进行故障排除。包含寡核苷酸 B2 对照品,用于为图像分析提供对齐信号。
仅供科研使用。不可用于诊断程序。
规格
适用于(应用)转录组谱分析
产品线GeneChip™
数量30 Reactions
试剂类型样品制备试剂
类型3' IVT PLUS 试剂盒
Unit SizeEach

常见问题解答 (FAQ)

What reagent kit should I use with my array?

Please refer to the Microarray Reagent Guide for Arrays and Expression Kits to match the correct reagents your array.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Where can I download the control sequences for expression arrays?

Sequences of all our controls can be downloaded from the sequence file that is available on product pages on thermofisher.com.

For example, here is the link to the product page for Clariom D Assay, human: https://www.thermofisher.com/order/catalog/product/902923
Go to the "Software & Data Analysis" section and under "Support Files", click on "Sequence Files: Clariom D Array, human Probe Sequences, tabular format", which you can open in Excel.

Note: If the control sequence file is not available, please contact techsupport@thermofisher.com.

Are pseudogene databases included in the design of expression arrays?

Pseudogene databases were not included in the design of expression arrays.

What is the shelf life of the Poly-A RNA Control's first dilution during processing of expression microarrays?

The first dilution of the Poly-A RNA Control can be stored at -20 degrees C for 6 weeks (with up to 4 freeze/thaws).

How long can I store labeled cDNA when working with expression microarrays?

Labeled material can be stored for 2 weeks at -20 degrees C.