ATTO-TAG™ FQ 衍生化试剂(FQ;3-(2-糠酰)喹啉-2-羧醛)
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Invitrogen™

ATTO-TAG™ FQ 衍生化试剂(FQ;3-(2-糠酰)喹啉-2-羧醛)

ATTO-TAG FQ 试剂允许对伯胺进行超灵敏检测。该试剂可作为独立产品或胺衍生化试剂盒 (A-2334) 的组分提供。ATTO-TAG FQ 在未反应状态下不发荧光,并且可与胺发生特异性反应形成高荧光偶联物,因此特别适合肽、氨基酸了解更多信息
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货号数量
A1019210 mg
货号 A10192
价格(CNY)
5,907.00
Each
添加至购物车
数量:
10 mg
请求批量或定制报价
价格(CNY)
5,907.00
Each
添加至购物车
ATTO-TAG FQ 试剂允许对伯胺进行超灵敏检测。该试剂可作为独立产品或胺衍生化试剂盒 (A-2334) 的组分提供。ATTO-TAG FQ 在未反应状态下不发荧光,并且可与胺发生特异性反应形成高荧光偶联物,因此特别适合肽、氨基酸、胺化糖和其他含胺分子的衍生化,以利用激光诱导荧光 (LIF) 进行毛细管电泳检测。最近,研究人员介绍了一种简单的方法,其中 ATTO-TAG FQ 试剂用于衍生高度稀释的肽溶液 (10-8 M)。
仅供科研使用。不可用于诊断程序。
规格
化学反应性
适用于(应用)伯胺检测
标签或染料ATTO-TAG FQ
数量10 mg
反应一部分醛类
运输条件室温
靶标伯胺
形式实心
产品线ATTO-TAG
类型衍生化试剂
Unit SizeEach
内容与储存
储存在冰箱(-5 至 -30°C)中并避光。

引用和文献 (18)

引用和文献
Abstract
Identification of proteins in single-cell capillary electrophoresis fingerprints based on comigration with standard proteins.
Authors:Hu S, Le Z, Newitt R, Aebersold R, Kraly JR, Jones M, Dovichi NJ,
Journal:Anal Chem
PubMed ID:14570203
'In the previous paper in this Journal, we reported the use of capillary sieving electrophoresis to characterize proteins expressed by single cancer cells at specific phases in the cell cycle. Analysis of the data revealed one component with cell cycle-dependent changes in expression at the 99% confidence limit. However, the ... More
Kinetics and apparent activation energy of the reaction of the fluorogenic reagent 5-furoylquinoline-3-carboxaldehyde with ovalbumin.
Authors:Pinto D, Arriaga EA, Schoenherr RM, Chou SS, Dovichi NJ
Journal:J Chromatogr B Analyt Technol Biomed Life Sci
PubMed ID:12880858
'Incomplete labeling of proteins by a derivatizing reagent usually results in the formation of a large number of products, which can produce unacceptable band broadening during electrophoretic analysis. In this paper, we report on the reaction of the fluorogenic reagent 5-furoylquinoline-3-carboxaldehyde (FQ) with the lysine residues of ovalbumin. Mass spectrometry ... More
Solid-phase fluorescent labeling reaction of picomole amounts of insulin in very dilute solutions and their analysis by capillary electrophoresis.
Authors:Pinto DM, Arriaga EA, Sia S, Li Z, Dovichi NJ
Journal:Electrophoresis
PubMed ID:7588523
'The fluorescent labeling of peptides at concentrations as low as 10(-8) M can be achieved by using a solid-phase reactor. Using oxidized insulin chain B as a test peptide, we demonstrate the use of an Immobilon CD membrane to capture and preconcentrate peptides. Insulin chain B can then be labeled ... More
MitoTracker Green labeling of mitochondrial proteins and their subsequent analysis by capillary electrophoresis with laser-induced fluorescence detection.
Authors:Presley AD, Fuller KM, Arriaga EA
Journal:J Chromatogr B Analyt Technol Biomed Life Sci
PubMed ID:12880861
'MitoTracker Green (MTG) is a mitochondrial-selective fluorescent label commonly used in confocal microscopy and flow cytometry. It is expected that this dye selectively accumulates in the mitochondrial matrix where it covalently binds to mitochondrial proteins by reacting with free thiol groups of cysteine residues. Here we demonstrate that MTG can ... More
Analysis of aminophospholipid molecular species by methyl-beta-cyclodextrin modified micellar electrokinetic capillary chromatography with laser-induced fluorescence detection.
Authors:Zhang L, Hu S, Cook L, Dovichi NJ
Journal:Electrophoresis
PubMed ID:12207317
'Micellar electrokinetic capillary chromatography (MEKC) with laser-induced fluorescence detection is used for the analysis of three classes of aminophospholipids: phosphatidylethanolamine (PE), phosphatidylserine (PS), and lysophosphatidylethanolamine (LPE) molecular species. 3-(2-Furoyl) quinoline-2-carboxaldehyde (FQ), a fluorogenic dye, was employed for labeling of these phospholipids. The FQ-labeled lipid species were then separated by sodium ... More