AbC™ 抗小鼠微珠试剂盒
AbC™ 抗小鼠微珠试剂盒
Invitrogen™

AbC™ 抗小鼠微珠试剂盒

The AbC™ Anti-Mouse Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry了解更多信息
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货号数量
A103441 kit
货号 A10344
价格(CNY)
4,269.00
飞享价
Ends: 31-Dec-2025
5,672.00
共减 1,403.00 (25%)
Each
添加至购物车
数量:
1 kit
价格(CNY)
4,269.00
飞享价
Ends: 31-Dec-2025
5,672.00
共减 1,403.00 (25%)
Each
添加至购物车
The AbC™ Anti-Mouse Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated mouse antibodies with any laser in flow cytometry.

Species reactivity—for use with all isotypes of mouse antibody conjugates
Maximum laser compatibility—for use with all lasers

Accurate compensation is crucial for effective multicolor analysis in flow cytometry. Often, however, cellular-based compensation controls are not completely effective, as many antigens are not highly expressed, and dimly stained cells can lead to inaccurate compensation settings. The AbC™ Anti-Mouse Bead Kit was developed to address this issue.

View a selection guide for all flow cytometry compensation beads.

How it works
Each kit provides polystyrene microspheres that have fluorescent antibody conjugate capture capacity (positive compensation beads) or are inert (negative compensation beads). After mixing with a fluorophore-conjugated mouse antibody, the two components provide a distinct high-signal positive control with an appropriate negative population that can then be used to set compensation properly regardless of the intensity of the cells in the actual experiment.

Species reactivity
The AbC™ Anti-Mouse Bead Kit is ideal for compensation with all isotypes of mouse (IgG1, IgG2a, IgG2b, IgG3, IgM) immunoglobulins.

Maximum laser compatibility
All AbC™ compensation bead kits are ideal for use with any excitation source, including the 405 nm laser which has historically provided higher background signals due to auto-fluorescence with other kits on the market.
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
直径(公制)6 μm
激发波长范围任意激光(UV 至 633/635)
适用于(设备)流式细胞仪
产品规格管装
测试数量100 tests
数量1 kit
运输条件室温
染色剂配置表面
产品线CompenFlow、Molecular Probes
产品类型补偿微珠
Unit SizeEach
内容与储存
包含 1 瓶 AbC™ 捕获微珠和 1 瓶阴性微珠。
  • 储存于 2°C 至 8°C
  • 常见问题解答 (FAQ)

    我在进行流式细胞实验时,补偿值超过100,可能出现这种情况吗?

    这是可能的。但是如果您的值超过100,可能需要查看你们用来采集数据的电压。

    有人告诉我说我需要补偿自己的流式细胞分析数据,这是什么意思?

    通过运行单色对照,有可能除去溢出到另一个荧光采集通道的荧光信号。

    流式细胞分析该选用哪种类型的对照?

    进行补偿时,你需要为自己使用的每种颜色参数准备单一染色样品(或补偿微球)。此外,我们建议您使用FMO(荧光扣除)对照。您可以使用减去一种颜色的其他所有颜色标记您的细胞或者微球来作为对照。为各种颜色准备FMO对照。这些对照对正确设置数据门限很有帮助。

    在流式细胞分析中为何要担心补偿?

    理想情况下,每一个单独荧光团的荧光发射光谱都非常集中,窄峰,发射峰之间分隔的较开。现实中,有机染料和荧光蛋白有很宽的发射峰,必须考虑补偿(数据采集中或后)来矫正任何一个荧光团荧光信号的分配。补偿从重叠光谱中移除了荧光信号,使您知道所看见的信号就是目标荧光团发出的信号,所以非常重要。

    如何为抗体设置补偿对照?

    补偿对照仅需未染色的细胞样品(或阴性对照)和单一颜色标记的样品——每管一个。如果您希望单色染色对照如您期望的那么亮,,那么可以使用我们的AbC总抗体补偿微珠试剂盒(货号A10513和A10497),这试剂盒是将抗体结合到微珠而非细胞上。