ACK 裂解缓冲液

ACK（铵-氯-钾）裂解缓冲液主要用于裂解含白细胞样品（比如用 EDTA 处理过的全血、血沉棕黄层和骨髓）中的红细胞。完整配方和裂解实验方案可在我们的技术参考库中找到。cGMP了解更多信息

货号	数量
A1049201	100 mL

货号 A1049201

价格（CNY)

421.00

添加至购物车

100 mL

价格（CNY)

421.00

添加至购物车

ACK（铵-氯-钾）裂解缓冲液主要用于裂解含白细胞样品（比如用 EDTA 处理过的全血、血沉棕黄层和骨髓）中的红细胞。完整配方和裂解实验方案可在我们的技术参考库中找到。

cGMP 生产和质量体系
ACK 裂解缓冲液在位于纽约格兰德岛上符合 cGMP 要求的工厂内生产。The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

仅供科研使用。不可用于诊断程序。

化学名称或材料裂解缓冲液

建议的储存条件储存条件：15–30°C.避光。
运输条件：常温
有效期：自生产之日起12个月

有效期12 个月

无菌无菌过滤

物理形态液体

产品线Gibco

数量100 mL

Unit SizeEach

常见问题解答 (FAQ)

What alternative do you offer for ACK Lysing Buffer (Cat. No. A1049201)?

Alternative products we offer are eBioscience 1X RBC Lysis Buffer (Cat. No. 00-4333-57) or eBioscience 10X RBC Lysis Buffer (Multi-species) (Cat. No. 00-4300-54).

Can I use ACK Lysing Buffer with heparinized blood?

Yes, ACK Lysing Buffer can be used with heparinized blood. The principle of lysis is osmotic treatment.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I use ACK Lysing Buffer to lyse red blood cells?

A volume of ACK lysing buffer corresponding to 10-20 times the volume of the blood sample is sufficient to lyse red blood cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

引用和文献 (1)

引用和文献

Abstract

Isolation of carbohydrate-specific CD4(+) T cell clones from mice after stimulation by two model glycoconjugate vaccines.

Authors:Avci FY, Li X, Tsuji M, Kasper DL,

Journal:Nat Protoc

PubMed ID:23196974

Here we describe how to isolate carbohydrate-specific T cell clones (for which we propose the designation 'Tcarbs') after stimulation by two glycoconjugate vaccines. We describe how to prepare, purify and characterize two model glycoconjugate vaccines that can be used to generate Tcarbs. These glycoconjugate vaccines (GBSIII-OVA and GBSIII-TT) are synthesized ... More