Amplex™ Red 单胺氧化酶检测试剂盒
Amplex™ Red 单胺氧化酶检测试剂盒
Invitrogen™

Amplex™ Red 单胺氧化酶检测试剂盒

Amplex Red 单胺氧化酶检测试剂盒提供了一种灵敏、简单的荧光检测单胺酶活性方法，在低至 12 μU/mL 的纯化酶制剂以及低至 20 μg 蛋白/100了解更多信息
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货号数量
A12214500 Assays
货号 A12214
价格（CNY)
6,650.00
Each
添加至购物车
数量:
500 Assays
价格（CNY)
6,650.00
Each
添加至购物车
Amplex Red 单胺氧化酶检测试剂盒提供了一种灵敏、简单的荧光检测单胺酶活性方法，在低至 12 μU/mL 的纯化酶制剂以及低至 20 μg 蛋白/100 μL 试验体积的组织样品中均适用。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型其他标记或染料
产品规格试管、96 孔板
数量500 Assays
运输条件室温
适用于（应用）单胺氧化酶测定试剂盒
适用于（设备）荧光计, 微孔板读数仪
产品线Amplex™
产品类型红色单胺氧化酶测定试剂盒
Unit SizeEach
内容与储存
在冷冻冰箱（-5°C 至 -30°C）中避光储存。

常见问题解答 (FAQ)

I'm using an Amplex Red kit, the reagent changes color to pink almost immediately in my own Krebs-Ringer buffer but not in HBSS. Why is this?

The components of Krebs-Ringer buffer (salts) should not cause oxidation of the Amplex reagent (which, in the presence of peroxidase and H2O2 oxidizes to resorufin, which is pink in color and fluorescent). Try water alone (the water used to make the Krebs-Ringer buffer). Since Hank's Buffered Saline Solution is typically purchased rather than made in the lab, it likely would not have the same contaminant. Another option is to degas the buffer prior to use to removed dissolved oxygen radicals.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can Amplex Red Assays be performed using cell lysates?

This is not recommended. The presence of endogenous proteases can complicate the assay by degrading the horseradish peroxidase (HRP). Endogenous peroxidases and antioxidants can modify the H2O2 required for the reaction, competing with HRP (and catalase) for the substrate.

The Amplex Red Assays are best performed with either purified enzymes or extracted H2O2 in a defined buffer system, extracellular solutions or body fluids (media, serum, etc.) that do not exhibit high levels of endogenous protease or oxidase activity and do not contain antioxidants.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.