Amplex™ Red 尿酸/尿酸酶检测试剂盒
Amplex™ Red 尿酸/尿酸酶检测试剂盒
引用和文献 (6)
Invitrogen™

Amplex™ Red 尿酸/尿酸酶检测试剂盒

Amplex™ Red 尿酸/尿酸酶检测试剂盒为检测尿酸或尿酸酶提供了一种灵敏而简单的方法，可利用荧光微孔板读数仪或荧光计进行检测。查看我们完整的荧光微孔板检测产品线。•检测到低至 100 nM 的尿酸浓度或低至 0.2 mU/mL了解更多信息
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货号数量
A22181400 Assays
货号 A22181
价格（CNY)
5,824.00
Each
添加至购物车
数量:
400 Assays
价格（CNY)
5,824.00
Each
添加至购物车
Amplex™ Red 尿酸/尿酸酶检测试剂盒为检测尿酸或尿酸酶提供了一种灵敏而简单的方法，可利用荧光微孔板读数仪或荧光计进行检测。

查看我们完整的荧光微孔板检测产品线。

•检测到低至 100 nM 的尿酸浓度或低至 0.2 mU/mL 的尿酸酶活性
•产品规格允许在多个时间点测量
•设计用于尽可能减少自发荧光干扰

血清尿酸是人体组织内嘌呤代谢的终产物，通过肾小球滤过经肾脏清除。大多数动物可以将尿酸代谢为更容易排泄的产物，但人类缺乏必需的酶——尿酸氧化酶（尿酸酶），原因在于人尿酸酶基因中存在两个“无义突变”。

Amplex™ Red 尿酸/尿酸酶检测试剂盒为检测尿酸或监测尿酸酶活性提供了一种超灵敏的方法。在检测中，尿酸酶催化尿酸转化为尿囊素、过氧化氢和二氧化碳。在存在辣根过氧化物酶 (HRP) 的情况下，过氧化氢与 Amplex™ Red 试剂发生化学计量反应，生成具有红色荧光的氧化产物试卤灵。

因为试卤灵的最大吸收和荧光发射波长分别约为 571 nm 和 585 nm，所以大多数生物样品的自发荧光对其几乎没有干扰。

Amplex™ Red 测定试剂盒可用于广泛的研究范围
可提供一系列经过验证的 Amplex™ Red 测定试剂盒进行细胞信号转导和脂质、神经生物学、炎症和免疫功能以及代谢方面的研究。我们还提供具有更高灵敏度和更明亮荧光的第二代试剂——Amplex™ UltraRed 试剂（货号 A36006）—具有更高灵敏度和更明亮荧光的第二代试剂以及 Amplex™ Red/UltraRed 终止试剂（货号：A33855）。Amplex™ Red/UltraRed 终止试剂允许使用者在确定的时间点轻松控制和终止荧光信号生成反应。加入终止试剂之后，荧光信号能够保持稳定状态至少 3 个小时。还提供定制检测设计和包装。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型其他标记或染料
产品规格管、96 孔板
数量400 Assays
运输条件室温
适用于（应用）尿酸/尿酸酶测定试剂盒
适用于（设备）微孔板读数仪, 分光光度计, 荧光计
产品线Amplex
产品类型红色尿酸/尿酸酶测定试剂盒
Unit SizeEach
内容与储存
在冷冻冰箱（-5°C 至 -30°C）中避光储存。

常见问题解答 (FAQ)

I'm using an Amplex Red kit, the reagent changes color to pink almost immediately in my own Krebs-Ringer buffer but not in HBSS. Why is this?

The components of Krebs-Ringer buffer (salts) should not cause oxidation of the Amplex reagent (which, in the presence of peroxidase and H2O2 oxidizes to resorufin, which is pink in color and fluorescent). Try water alone (the water used to make the Krebs-Ringer buffer). Since Hank's Buffered Saline Solution is typically purchased rather than made in the lab, it likely would not have the same contaminant. Another option is to degas the buffer prior to use to removed dissolved oxygen radicals.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can Amplex Red Assays be performed using cell lysates?

This is not recommended. The presence of endogenous proteases can complicate the assay by degrading the horseradish peroxidase (HRP). Endogenous peroxidases and antioxidants can modify the H2O2 required for the reaction, competing with HRP (and catalase) for the substrate.

The Amplex Red Assays are best performed with either purified enzymes or extracted H2O2 in a defined buffer system, extracellular solutions or body fluids (media, serum, etc.) that do not exhibit high levels of endogenous protease or oxidase activity and do not contain antioxidants.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (6)

引用和文献
Abstract
Use of colorimetric test strips for monitoring the effect of hemodialysis on salivary nitrite and uric acid in patients with end-stage renal disease: a proof of principle.
Authors:Blicharz TM, Rissin DM, Bowden M, Hayman RB, DiCesare C, Bhatia JS, Grand-Pierre N, Siqueira WL, Helmerhorst EJ, Loscalzo J, Oppenheim FG, Walt DR,
Journal:Clin Chem
PubMed ID:18676588
'BACKGROUND: Initial screening of potential biomarkers for monitoring dialysis was performed with saliva samples collected from patients with end-stage renal disease (ESRD). A more thorough analysis of the most promising markers identified in the initial screening was conducted with saliva samples acquired at hourly intervals throughout dialysis to monitor analyte ... More
HucR, a novel uric acid-responsive member of the MarR family of transcriptional regulators from Deinococcus radiodurans.
Authors:Wilkinson SP, Grove A,
Journal:J Biol Chem
PubMed ID:15448166
'The MarR family of transcriptional regulators comprises a subset of winged helix DNA-binding proteins and includes numerous members that function in environmental surveillance of aromatic compounds. We describe the characterization of HucR, a novel MarR homolog from Deinococcus radiodurans that demonstrates phenolic sensing capabilities. HucR binds as a homodimer to ... More
Fructose induces transketolase flux to promote pancreatic cancer growth.
Authors:Liu H, Huang D, McArthur DL, Boros LG, Nissen N, Heaney AP,
Journal:Cancer Res
PubMed ID:20647326
'Carbohydrate metabolism via glycolysis and the tricarboxylic acid cycle is pivotal for cancer growth, and increased refined carbohydrate consumption adversely affects cancer survival. Traditionally, glucose and fructose have been considered as interchangeable monosaccharide substrates that are similarly metabolized, and little attention has been given to sugars other than glucose. However, ... More
Activation of NLRP3 inflammasome in alveolar macrophages contributes to mechanical stretch-induced lung inflammation and injury.
Authors:Wu J, Yan Z, Schwartz DE, Yu J, Malik AB, Hu G,
Journal:J Immunol
PubMed ID:23436933
Mechanical ventilation of lungs is capable of activating the innate immune system and inducing sterile inflammatory response. The proinflammatory cytokine IL-1ß is among the definitive markers for accurately identifying ventilator-induced lung inflammation. However, mechanisms of IL-1ß release during mechanical ventilation are unknown. In this study, we show that cyclic stretch ... More
IL-1R1/MyD88 signaling is critical for elastase-induced lung inflammation and emphysema.
Authors:Couillin I, Vasseur V, Charron S, Gasse P, Tavernier M, Guillet J, Lagente V, Fick L, Jacobs M, Coelho FR, Moser R, Ryffel B,
Journal:J Immunol
PubMed ID:20007584
Lung emphysema and fibrosis are severe complications of chronic obstructive pulmonary disease, and uncontrolled protease activation may be involved in the pathogenesis. Using experimental elastase-induced acute inflammation, we demonstrate here that inflammation and development of emphysema is IL-1R1 and Toll/IL-1R signal transduction adaptor MyD88 dependent; however, TLR recognition is dispensable ... More
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