Amplex™ Red 过氧化氢/过氧化物酶检测试剂盒
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Amplex™ Red 过氧化氢/过氧化物酶检测试剂盒
Amplex™ Red 过氧化氢/过氧化物酶检测试剂盒
Amplex™ Red 过氧化氢/过氧化物酶检测试剂盒
Amplex™ Red 过氧化氢/过氧化物酶检测试剂盒
Invitrogen™

Amplex™ Red 过氧化氢/过氧化物酶检测试剂盒

Amplex™ Red 过氧化氢/过氧化物酶测定试剂盒包含灵敏的一步法测定试剂盒,该测定试剂盒使用 Amplex™ Red 试剂(10-乙酰基-3,7-二羟基吩噁嗪)检测过氧化氢 (H2O2) 或过氧化物酶活性了解更多信息
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货号数量
A22188500 Assays
货号 A22188
价格(CNY)
6,469.00
Each
添加至购物车
数量:
500 Assays
价格(CNY)
6,469.00
Each
添加至购物车
Amplex™ Red 过氧化氢/过氧化物酶测定试剂盒包含灵敏的一步法测定试剂盒,该测定试剂盒使用 Amplex™ Red 试剂(10-乙酰基-3,7-二羟基吩噁嗪)检测过氧化氢 (H2O2) 或过氧化物酶活性。Amplex™ Red 试剂与辣根过氧化物酶 (HRP) 配合使用以检测从生物样品(包括细胞)中释放或在酶偶联反应中生成的 H2O2。此外,当 H2O2 过量时,Amplex™ Red 试剂也可用作过氧化物酶活性的超灵敏测定试剂。在存在过氧化物酶的情况下,Amplex™ Red 试剂与 H2O2 以 1:1 的化学计量比发生反应,生成红色荧光氧化产物试卤灵。试卤灵的上限激发和发射波长分别约为 571 nm 和 585 nm,由于消光系数较高 (58,000 ± 5,000 cm–1M–1),您可通过荧光法或分光光度法进行该测定。该反应用于检测 100 µL 体积或 1 x 10–5 U/mL HRP 中低至10皮摩尔的 H2O2

Amplex™ Red 过氧化氢/过氧化物酶测定试剂盒规格:
• 反应产物试卤灵的激发/发射波长:∼约 571/585 nm
•用于在体外检测过氧化氢或过氧化物酶的灵敏一步法测定试剂盒
•可在 100 µL 测定容积中检测出低至10皮摩尔的过氧化氢或 10 µU/mL 辣根过氧化物酶活性
• 提供足够用于约500次测定的试剂,每次测定的反应体积为 100 µL
• 设计用于96孔荧光或吸光度微孔板读数仪,但可轻松适应荧光计和分光光度计的其他容积


查找更多氧化酶底物
有关这些产品的更多信息,请查看氧化酶底物,包括 Amplex Red 试剂盒 — Molecular Probes™ 手册中 10.5 节

仅供科研使用。不可用于人或动物的治疗或诊断。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型其他标记或染料
产品规格管、96 孔板
数量500 Assays
运输条件室温
适用于(应用)过氧化氢/过氧化物酶测定试剂盒
适用于(设备)微孔板读数仪, 分光光度计, 荧光计
产品线Amplex™
产品类型红色过氧化氢/过氧化物酶测定试剂盒
Unit SizeEach
内容与储存
在冷冻冰箱(-5°C 至 -30°C)中避光储存。

常见问题解答 (FAQ)

I'm using an Amplex Red kit, the reagent changes color to pink almost immediately in my own Krebs-Ringer buffer but not in HBSS. Why is this?

The components of Krebs-Ringer buffer (salts) should not cause oxidation of the Amplex reagent (which, in the presence of peroxidase and H2O2 oxidizes to resorufin, which is pink in color and fluorescent). Try water alone (the water used to make the Krebs-Ringer buffer). Since Hank's Buffered Saline Solution is typically purchased rather than made in the lab, it likely would not have the same contaminant. Another option is to degas the buffer prior to use to removed dissolved oxygen radicals.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit (Cat. No. A22188) to measure H2O2 from frozen cell culture supernatant?

We recommend using fresh samples that have not been frozen since H2O2 degrades over time and more readily in diluted form or in solutions that contain metal ions or natural antioxidants. For assaying cell culture supernatant, we would recommend analyzing samples as soon as possible.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can Amplex Red Assays be performed using cell lysates?

This is not recommended. The presence of endogenous proteases can complicate the assay by degrading the horseradish peroxidase (HRP). Endogenous peroxidases and antioxidants can modify the H2O2 required for the reaction, competing with HRP (and catalase) for the substrate.

The Amplex Red Assays are best performed with either purified enzymes or extracted H2O2 in a defined buffer system, extracellular solutions or body fluids (media, serum, etc.) that do not exhibit high levels of endogenous protease or oxidase activity and do not contain antioxidants.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.