Amplex™ Red 葡萄糖/葡萄糖氧化酶检测试剂盒
Amplex™ Red 葡萄糖/葡萄糖氧化酶检测试剂盒
引用和文献 (21)
Invitrogen™

Amplex™ Red 葡萄糖/葡萄糖氧化酶检测试剂盒

Amplex™ Red 葡萄糖/葡萄糖氧化酶检测试剂盒为检测 d-葡萄糖或葡萄糖氧化酶提供了一种灵敏而简单的方法，可利用荧光微孔板读数仪或荧光计进行检测。查看我们完整的荧光微孔板检测产品线。•检测到低至 3 µM 的 d-葡萄糖浓度或低至了解更多信息
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货号数量
A22189500 Assays
货号 A22189
价格（CNY)
5,930.00
Each
添加至购物车
数量:
500 Assays
价格（CNY)
5,930.00
Each
添加至购物车
Amplex™ Red 葡萄糖/葡萄糖氧化酶检测试剂盒为检测 d-葡萄糖或葡萄糖氧化酶提供了一种灵敏而简单的方法，可利用荧光微孔板读数仪或荧光计进行检测。

查看我们完整的荧光微孔板检测产品线。

•检测到低至 3 µM 的 d-葡萄糖浓度或低至 0.05 mU/mL 的葡萄糖氧化酶活性
•产品规格允许在多个时间点测量
•设计用于尽可能减少自发荧光干扰

Amplex™ Red 试剂（10-乙酰基-3,7-二羟基吩嗪）是一种无色、稳定且用途极其广泛的过氧化物酶底物。由于过氧化物酶和葡萄糖氧化酶介导的反应可以偶联，因此可以在连续或不连续测定中通过任何葡糖苷酶（例如，β-葡糖苷酶和葡糖脑苷脂酶）测量葡萄糖氧化酶活性或葡萄糖释放量。因为可以偶联这些反应，所以该检测方法已被证明可用于食品、发酵培养基和体液中葡萄糖含量的定量测定。

在检测中，葡萄糖氧化酶与 d-葡萄糖发生反应形成 d-葡糖酸内酯和过氧化氢。在存在辣根过氧化物酶 (HRP) 的情况下，过氧化氢与 Amplex™ Red 试剂以 1:1 的化学计量比发生反应，生成红色荧光产物试卤灵。

因为试卤灵的最大吸收和荧光发射波长分别约为 571 nm 和585 nm，所以大多数生物样品的自发荧光对其几乎没有干扰。

Amplex™ Red 测定试剂盒可用于广泛的研究范围
可提供一系列经过验证的 Amplex™ Red 测定试剂盒进行细胞信号转导和脂质、神经生物学、炎症和免疫功能以及代谢方面的研究。我们还提供具有更高灵敏度和更明亮荧光的第二代试剂——Amplex™ UltraRed 试剂（货号 A36006）—具有更高灵敏度和更明亮荧光的第二代试剂以及 Amplex™ Red/UltraRed 终止试剂（货号：A33855）。Amplex™ Red/UltraRed 终止试剂允许使用者在确定的时间点轻松控制和终止荧光信号生成反应。加入终止试剂之后，荧光信号能够保持稳定状态至少 3 个小时。还提供定制检测设计和包装。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型其他标记或染料
产品规格管、96 孔板
数量500 Assays
运输条件室温
适用于（应用）葡萄糖/葡萄糖氧化酶测定试剂盒
适用于（设备）微孔板读数仪, 分光光度计, 荧光计
产品线Amplex
产品类型红色葡萄糖/葡萄糖氧化酶测定试剂盒
Unit SizeEach
内容与储存
在冷冻冰箱（-5°C 至 -30°C）中避光储存。

常见问题解答 (FAQ)

I'm using an Amplex Red kit, the reagent changes color to pink almost immediately in my own Krebs-Ringer buffer but not in HBSS. Why is this?

The components of Krebs-Ringer buffer (salts) should not cause oxidation of the Amplex reagent (which, in the presence of peroxidase and H2O2 oxidizes to resorufin, which is pink in color and fluorescent). Try water alone (the water used to make the Krebs-Ringer buffer). Since Hank's Buffered Saline Solution is typically purchased rather than made in the lab, it likely would not have the same contaminant. Another option is to degas the buffer prior to use to removed dissolved oxygen radicals.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can Amplex Red Assays be performed using cell lysates?

This is not recommended. The presence of endogenous proteases can complicate the assay by degrading the horseradish peroxidase (HRP). Endogenous peroxidases and antioxidants can modify the H2O2 required for the reaction, competing with HRP (and catalase) for the substrate.

The Amplex Red Assays are best performed with either purified enzymes or extracted H2O2 in a defined buffer system, extracellular solutions or body fluids (media, serum, etc.) that do not exhibit high levels of endogenous protease or oxidase activity and do not contain antioxidants.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (21)

引用和文献
Abstract
Development of a long-acting insulin analog using albumin fusion technology.
Authors:Duttaroy A, Kanakaraj P, Osborn BL, Schneider H, Pickeral OK, Chen C, Zhang G, Kaithamana S, Singh M, Schulingkamp R, Crossan D, Bock J, Kaufman TE, Reavey P, Carey-Barber M, Krishnan SR, Garcia A, Murphy K, Siskind JK, McLean MA, Cheng S, Ruben S, Birse CE, Blondel O
Journal:Diabetes
PubMed ID:15616036
The primary therapeutic goal for the treatment of diabetes is maintenance of a long-term, near-normoglycemic condition and prevention of the onset or progression of the complications associated with the disease. Although several analogs of human insulin have been developed, the currently prescribed long-acting insulin analogs do not provide a stable ... More
Perturbational profiling of a cell-line model of tumorigenesis by using metabolic measurements.
Authors:Ramanathan A, Wang C, Schreiber SL
Journal:Proc Natl Acad Sci U S A
PubMed ID:15840712
'Weinberg and coworkers have used serial transduction of a human, primary fibroblast cell line with the catalytic domain of human telomerase, large T antigen, small T antigen, and an oncogenic allele of H-ras to study stages leading toward a fully transformed cancerous state. We performed a three-dimensional screening experiment using ... More
Irs1 and Irs2 signaling is essential for hepatic glucose homeostasis and systemic growth.
Authors:Dong X, Park S, Lin X, Copps K, Yi X, White MF
Journal:J Clin Invest
PubMed ID:16374520
'Insulin receptor substrates, including Irs1 and Irs2, integrate insulin and IGF receptor signals with heterologous pathways to coordinate growth and metabolism. Since Irs2 is thought to be especially important in hepatic nutrient homeostasis, we deleted Irs2 [corrected] from hepatocytes of WT mice (called LKO) or genetically insulin-resistant Irs1-/- mice (called ... More
Stabilization of enzymes in silk films.
Authors:Lu S, Wang X, Lu Q, Hu X, Uppal N, Omenetto FG, Kaplan DL,
Journal:Biomacromolecules
PubMed ID:19323497
'Material systems are needed that promote stabilization of entrained molecules, such as enzymes or therapeutic proteins, without destroying their activity. We demonstrate that the unique structure of silk fibroin protein, when assembled into the solid state, establishes an environment that is conducive to the stabilization of entrained proteins. Enzymes (glucose ... More
Inhibition of mitochondrial pyruvate transport by zaprinast causes massive accumulation of aspartate at the expense of glutamate in the retina.
Authors:Du J, Cleghorn WM, Contreras L, Lindsay K, Rountree AM, Chertov AO, Turner SJ, Sahaboglu A, Linton J, Sadilek M, Satrústegui J, Sweet IR, Paquet-Durand F, Hurley JB,
Journal:
PubMed ID:24187136
'Transport of pyruvate into mitochondria by the mitochondrial pyruvate carrier is crucial for complete oxidation of glucose and for biosynthesis of amino acids and lipids. Zaprinast is a well known phosphodiesterase inhibitor and lead compound for sildenafil. We found Zaprinast alters the metabolomic profile of mitochondrial intermediates and amino acids ... More
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