The Ion AmpliSeq™ Kit for Chef DL8 is designed for use with the Ion Chef™ System for automation of Ion AmpliSeq library construction.
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货号
数量
A29024
32 Reactions
货号 A29024
价格(CNY)
-
数量:
32 Reactions
The Ion AmpliSeq™ Kit for Chef DL8 is designed for use with the Ion Chef™ System for automation of Ion AmpliSeq library construction.
This kit offers:
Simplest workflow for targeted sequencing library preparation using 1 or 2 pool Ion AmpliSeq panel designs*
Low input
Easy-to-use cartridge format with reagent barcoding for full reagent tracking
Proven performance of Ion AmpliSeq technology, compatible with DNA extracted from formalin-fixed, paraffin-embedded tissue and other challenging sources
Chef DL8 的 Ion AmpliSeq™ 试剂盒设计用于配合 Ion Chef™ 系统使用,用于自动化构建 Ion AmpliSeq 文库。该试剂盒可提供:
•使用1或2池 Ion AmpliSeq 检测组合设计,可获得极简单的靶向测序文库制备工作流程* • 低起始量 • 带试剂条形码的易于使用的纯化柱形式可用于全试剂示踪 • Ion AmpliSeq 技术的性能经证实,与从福尔马林固定石蜡包埋组织和其他挑战性来源中提取的 DNA 兼容
利用 Ion Chef 系统的自动化功能,用于 Chef DL8 的 Ion AmpliSeq 试剂盒能可重现地制备 Ion AmpliSeq 扩增子文库,手动操作时间少于 15 分钟并且仅需 3 个液体转移步骤。该试剂盒与 1 池和 2 池 Ion AmpliSeq panel 的设计兼容,可在一次运行中自动制备 8 份样本的文库,同时提供一管可用于下游模板构建和测序的混合文库。该试剂盒提供用于制备 32 个文库(4 次 Ion Chef 运行)的充足材料。
What is the difference between a DNA fragment library and a DNA amplicon library?
A DNA fragment library is constructed from whole genomic DNA and is commonly used for whole genome resequencing or de novo sequencing. Briefly, the whole genomic DNA is fragmented or sheared, ligated with Ion-specific adapter sequences, and then size-selected for the library fragments of the desired length.
Amplicon libraries are constructed from PCR-amplified DNA fragments and are used for targeted sequencing (e.g., investigating variants at known genomic locations). There are two types of amplicon libraries, short and long.
A short amplicon library contains DNA fragments (targets) with lengths that are compatible with the Ion template preparation kits without any further shearing or fragmentation during library preparation. Additionally, no size-selection step is required, as the amplicons are already within the desired size range.
A long amplicon library contains DNA fragments (targets) with lengths that are longer than those compatible with the Ion template kits and requires further shearing or fragmentation during library preparation. The library preparation protocol for long amplicons is similar to fragment libraries.
What do I need to do to prep the barcode adapters in the IonCode Barcode Adapters 1-96 Kit (Cat. No. A29747)?
When handling barcode adapters, be careful to avoid cross-contamination by changing gloves frequently and by carefully removing the plate seal.
- Pulse vortex the IonCode Barcode Adapters plate several times.
- Centrifuge the IonCode Barcode Adapters plate for 1 minute at 1000 x g to collect contents.
- Carefully remove, then discard the plate seal.
How many reactions are possible with the IonCode Barcode Adapters 1-96 Kit (Cat. No. A29747)?
The IonCode Barcode Adapters 1-96 Kit consists of 1 x 96-well plate, with 20 µL of each barcode per well. The plate consists of 960 reactions in total with 10 reactions for each of the 96 barcodes.
In what format are the barcode adapters in the IonCode Barcode Adapters 1-96 Kit (Cat. No. A29747)?
The IonCode Barcode Adapters 1-96 Kit consists of 1 x 96-well plate, with 20 µL of each barcode per well. The barcodes are pre-diluted, ready-to-use. The 96-well plate format allows multichannel pipetting for more efficient work.