用于 Chef DL8 的 Ion AmpliSeq™ 试剂盒
用于 Chef DL8 的 Ion AmpliSeq™ 试剂盒
Ion Torrent™

用于 Chef DL8 的 Ion AmpliSeq™ 试剂盒

Chef DL8 的 Ion AmpliSeq™ 试剂盒设计用于配合 Ion Chef™ 系统使用,用于自动化构建 Ion了解更多信息
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货号数量
A2902432 Reactions
货号 A29024
价格(CNY)
-
数量:
32 Reactions
Chef DL8 的 Ion AmpliSeq™ 试剂盒设计用于配合 Ion Chef™ 系统使用,用于自动化构建 Ion AmpliSeq 文库。该试剂盒可提供:

•使用1或2池 Ion AmpliSeq 检测组合设计,可获得极简单的靶向测序文库制备工作流程*
• 低起始量
• 带试剂条形码的易于使用的纯化柱形式可用于全试剂示踪
• Ion AmpliSeq 技术的性能经证实,与从福尔马林固定石蜡包埋组织和其他挑战性来源中提取的 DNA 兼容

*有关定制 1-2 池检测组合,参见 AmpliSeq.cn。请注意,某些检测组合(例如转录组癌症热点Oncomine BRCA药物基因组学)通过将相应的即用型引物池加入 DL8 试剂盒在 Ion Chef-ready 试剂盒中制备。

利用 Ion Chef 系统的自动化功能,用于 Chef DL8 的 Ion AmpliSeq 试剂盒能可重现地制备 Ion AmpliSeq 扩增子文库,手动操作时间少于 15 分钟并且仅需 3 个液体转移步骤。该试剂盒与 1 池和 2 池 Ion AmpliSeq panel 的设计兼容,可在一次运行中自动制备 8 份样本的文库,同时提供一管可用于下游模板构建和测序的混合文库。该试剂盒提供用于制备 32 个文库(4 次 Ion Chef 运行)的充足材料。

利用全自动化工作流程的即插即用、预包装、单次使用纯化柱和 PCR 板,各种经验水平的用户均可轻松制备文库,工作量极小。提供在 96 孔板中方便吸干的条形码,多通路复用样品过程简单且无需额外移液步骤。

有关 Ion AmpliSeq 技术的更多信息 >
规格
适用于(应用)测序
适用于(设备)Ion GeneStudio S5 系统、Ion GeneStudio™ S5 系统、Ion PGM™ 系统、Ion Proton™ 系统、Ion S5™ XL 系统、Ion OneTouch™ 2 系统、Ion Chef™ 系统
靶向测序库
反应次数32 Reactions
产品线AmpliSeq™
产品类型DNA 测序试剂盒
数量32 Reactions
样品类型DNA
运输条件干冰
工作流程步骤文库生成
产品规格试剂盒
测序类型基因组和 DNA 测序
Unit SizeEach
内容与储存
• 4 个纯化柱 Ion AmpliSeq Chef 试剂 DL8,在 -5 至 -30°C 下储存
•4 个纯化柱 Ion AmpliSeq Chef 溶液 DL8,在 2–8°C
下储存•4 盒 Ion AmpliSeq 用品(每盒包含:Ion AmpliSeq 吸头纯化柱 L8、盒装 PCR 铝箔封口、富集纯化柱),在室温下储存
•1 套 PCR 板中的 4 IonCode(该套件包括:96 孔 PCR 板中 IonCode 0101-0108(红色)、96 孔 PCR 板中 IonCode 0109-0116(黄色)、96 孔 PCR 板中 IonCode 0117-0124(绿色)、96 孔 PCR 板中 IonCode 0125-0132(蓝色),在室温下储存

常见问题解答 (FAQ)

What is the difference between a DNA fragment library and a DNA amplicon library?

A DNA fragment library is constructed from whole genomic DNA and is commonly used for whole genome resequencing or de novo sequencing. Briefly, the whole genomic DNA is fragmented or sheared, ligated with Ion-specific adapter sequences, and then size-selected for the library fragments of the desired length.

Amplicon libraries are constructed from PCR-amplified DNA fragments and are used for targeted sequencing (e.g., investigating variants at known genomic locations). There are two types of amplicon libraries, short and long.

A short amplicon library contains DNA fragments (targets) with lengths that are compatible with the Ion template preparation kits without any further shearing or fragmentation during library preparation. Additionally, no size-selection step is required, as the amplicons are already within the desired size range.

A long amplicon library contains DNA fragments (targets) with lengths that are longer than those compatible with the Ion template kits and requires further shearing or fragmentation during library preparation. The library preparation protocol for long amplicons is similar to fragment libraries.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What do I need to do to prep the barcode adapters in the IonCode Barcode Adapters 1-96 Kit (Cat. No. A29747)?

When handling barcode adapters, be careful to avoid cross-contamination by changing gloves frequently and by carefully removing the plate seal.
- Pulse vortex the IonCode Barcode Adapters plate several times.
- Centrifuge the IonCode Barcode Adapters plate for 1 minute at 1000 x g to collect contents.
- Carefully remove, then discard the plate seal.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many reactions are possible with the IonCode Barcode Adapters 1-96 Kit (Cat. No. A29747)?

The IonCode Barcode Adapters 1-96 Kit consists of 1 x 96-well plate, with 20 µL of each barcode per well. The plate consists of 960 reactions in total with 10 reactions for each of the 96 barcodes.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

In what format are the barcode adapters in the IonCode Barcode Adapters 1-96 Kit (Cat. No. A29747)?

The IonCode Barcode Adapters 1-96 Kit consists of 1 x 96-well plate, with 20 µL of each barcode per well. The barcodes are pre-diluted, ready-to-use. The 96-well plate format allows multichannel pipetting for more efficient work.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What are the shipping and storage conditions for IonCode Barcode Adapters 1-96 Kit (Cat. No. A29747)?

The IonCode Barcode Adapters 1-96 Kit is shipped on dry ice and we recommend storing at -30 degrees C to -5 degrees C in an upright position.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.