ExpiCHO™ 表达培养基
Invitrogen17万+抗体限时买二赠一，靶点广，灵活用！
ExpiCHO™ 表达培养基
Gibco™

ExpiCHO™ 表达培养基

ExpiCHO™ 表达培养基是按照 GMP 标准生产且化学成分确定的无血清、无蛋白且无动物来源的培养基，专为悬浮适应的中国仓鼠卵巢 (CHO) 细胞的高密度生长及转染而设计。它是 ExpiCHO™ 表达系统试剂盒了解更多信息
Have Questions?
更改视图buttonViewtableView
货号数量
A29100026 x 1 L
A29100011000 mL
A291000420 L
A291000310 L
货号 A2910002
价格（CNY)
32,840.00
Each
添加至购物车
数量:
6 x 1 L
价格（CNY)
32,840.00
Each
添加至购物车
ExpiCHO™ 表达培养基是按照 GMP 标准生产且化学成分确定的无血清、无蛋白且无动物来源的培养基，专为悬浮适应的中国仓鼠卵巢 (CHO) 细胞的高密度生长及转染而设计。它是 ExpiCHO™ 表达系统试剂盒（货号 A29133）的核心组分，用于可扩展的瞬时转染蛋白生产。

ExpiCHO 表达培养基：
•支持悬浮 CHO 培养物的生长直至密度超过 2 x 107 个细胞/mL，同时保持细胞活力 > 95%
• 使用兼容转染的无血清悬浮培养基，配合 ExpiFectamine™ CHO 转染试剂可实现 > 90% 的高转染效率
• 支持在 6 x 106 个细胞/mL 的密度下瞬时转染悬浮 CHO 细胞
• 促进高密度瞬时转染培养物的持续高水平表达，从而实现高达 3 g/L 的重组蛋白得率
• 支持 CHO 细胞在各种培养方式下的生长和转染，从多孔板中的 <1 mL 到台式生物反应器中的 >10 L
• 不含酚红

ExpiCHO 表达培养基配方中包含 GlutaMAX™-I 试剂。可直接使用，无需添加剂。化学成分明确的配方可实现高度的重现性和批间可靠性。为了获得较高水平的瞬时蛋白表达，该培养基应与匹配的培养基补料——ExpiCHO™ 补料配合使用，后者作为 ExpiFectamine™ CHO 转染试剂盒（货号 A29129）。不推荐将 ExpiCHO 表达培养基用于贴壁细胞培养。细胞通常置于锥形瓶内，放在摇床平台上，然后放入加湿的 CO2 细胞培养箱内生长。但是培养体积可以从多孔板中的 <1 mL 扩展到生物反应器中的 >10 L。
规格
细胞系CHO-S、SFM 适用
细胞类型Chinese hamster ovary (CHO) cells
最大浓度1X
表达系统ExpiCHO-S™
产品规格瓶装
产品线ExpiCHO™
产品类型CHO 表达培养基
数量6 x 1 L
有效期12 Months
运输条件室温
分类非动物源性, 化学成分明确, 无蛋白, 无血清
培养类型悬浮液细胞培养
形式液体
血清水平无血清
无菌Sterile
灭菌方法Sterile-filtered
加有添加剂GlutaMAX
不加添加剂无酚红
Unit SizeEach
内容与储存
储存条件：储存于 2-8°C 的阴暗处
运输条件：环境温度
有效期：自生产之日起12个月

常见问题解答 (FAQ)

My ExpiCHO Expression Medium (Cat. No. A2910002) was accidentally frozen. Can I still use it?

This product should be stored at 2-8 degrees C and should not be frozen. The biggest issue with accidentally freezing this product is its solubility. If this product was accidentally frozen, we recommend placing it in a 2-8 degree environment and allowing it to slowly thaw overnight. If the product is fully thawed and shows no signs of precipitation, then it should still be usable, but we cannot guarantee effectiveness.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

When subculturing ExpiCHO the day before a transfection to 3-4 x 10E6/mL, would you recommend pelleting the cells and resuspending them in completely fresh media, or simply replacing some of the media to split them down to the desired cell density?

Before transfecting ExpiCHO cells, there is no need to change out the media during the subculturing. This would become expensive and the conditioned media is not necessary.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

For how many passages would you recommend using ExpiCHO cells before replacing them with new cells that were frozen at earlier passages?

For optimal performance with respect to protein production, we recommend thawing ExpiCHO cells and letting them grow for 2-3 passages prior to transfection. Overall, we do not recommend using the cells beyond ˜20 passages.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How many days should I wait before harvesting my protein with the ExpiCHO system?

Optimal time to harvest protein will depend on the specific properties of the protein being expressed and the protocol chosen. Typical harvest times to reach maximum titers for the various protocols are as follows:
- Standard Protocol: 8-10 days post-transfection
- High Titer Protocol: 10-12 days post-transfection
- Max Titer Protocol: 12-14 days post-transfection

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

I didn't add the enhancer with the ExpiCHO system. Will I still get good protein expression?

The Enhancer is designed to work with the ExpiCHO system for maximal expression. If the enhancer is not added, there will be a greater than 50% loss in expression.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

View all ExpiCHO™ 表达培养基 FAQs