Alexa Fluor™ 488 5-SDP 酯(Alexa Fluor™ 488 Sulfodichlorophenol 酯)
Alexa Fluor™ 488 5-SDP 酯(Alexa Fluor™ 488 Sulfodichlorophenol 酯)
Invitrogen™

Alexa Fluor™ 488 5-SDP 酯(Alexa Fluor™ 488 Sulfodichlorophenol 酯)

SDP (sulfodicholorphenol) 酯是目前为止 Invitrogen 提供的水解时最稳定的胺反应性部分。通过 Alexa Fluor™ 488 5-SDP了解更多信息
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货号数量
A300521 mg
货号 A30052
价格(CNY)
6,260.00
Each
添加至购物车
数量:
1 mg
价格(CNY)
6,260.00
Each
添加至购物车
SDP (sulfodicholorphenol) 酯是目前为止 Invitrogen 提供的水解时最稳定的胺反应性部分。通过 Alexa Fluor™ 488 5-SDP 酯生成的偶联物产生的染料和目标化合物间酰胺键的强度与琥珀酰亚胺(SE 或 NHS)和四氟苯基 (TFP) 酯间酰胺键的强度相同,但是与 NHS 和 TFP 酯相比,随着 SDP 酯在水和缓冲液中的稳定性得到改善,其可能会增强对这些反应的控制和这些反应的一致性。
仅供科研使用。不可用于诊断程序。
规格
化学反应性
标签或染料Alexa Fluor™ 488
产品类型Sulfodichlorophenol 酯
数量1 mg
反应一部分活性酯,SDP 酯
运输条件室温
标签类型Alexa Fluor 染料
产品线Alexa Fluor™
Unit SizeEach
内容与储存
储存在冰箱(-5 至 -30°C)中并避光。

常见问题解答 (FAQ)

I am labeling a protein with Alexa Fluor 488 SDP ester. The manual recommends using a sodium bicarbonate buffer at pH 8.3. Can I use a different buffer instead?

Yes. The important thing is to use a buffered solution with a pH between 8.0 and 8.5. Do not use Tris buffer, which has amine groups. Most other buffers will work fine in that pH range. This is also true for other amine-reactive dyes, such as succinimidyl (NHS) esters or TFP esters.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am not going to use all of my Alexa Fluor succinimidyl ester reactive dye. Can I just make it up in DMSO and store aliquots at -20 degrees C?

This is not recommended. Any trace amounts of water in the DMSO can promote spontaneous hydrolysis over time. Even if using anhydrous DMSO, DMSO is hygroscopic; it readily absorbs moisture from the atmosphere over time. A better alternative is to dissolve the reactive dye in a volatile solvent, make smaller aliquots and then evaporate off the solvent using a vacuum pump. The smaller aliquots of solid reactive dye should then be stored frozen, desiccated and protected from light. Contact Technical Support by sending an email to techsupport@thermofisher.com for the recommended volatile solvent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.