ReproSeq™ PGS 316 View 试剂盒(含芯片)
ReproSeq™ PGS 316 View 试剂盒(含芯片)
Ion Torrent™

ReproSeq™ PGS 316 View 试剂盒(含芯片)

ReproSeq™ PGS View 试剂盒为非整倍性分析提供了快速台式下一代测序 (NGS) 工作流程。该试剂盒与 Ion PGM™ 系统联用,能够快速经济地检测所有了解更多信息
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货号数量
A32249120 samples
货号 A32249
价格(CNY)
-
数量:
120 samples
ReproSeq™ PGS View 试剂盒为非整倍性分析提供了快速台式下一代测序 (NGS) 工作流程。该试剂盒与 Ion PGM™ 系统联用,能够快速经济地检测所有 24 条染色体(22 条常染色体 + X 染色体 + Y 染色体)的非整倍性,以便实验室能够快速、可靠地评估植入前胚胎以进行研究。ReproSeq PGS View 试剂盒利用从单个细胞中提取的低至 6 pg DNA,即可进行全染色体与染色体臂事件 (≥48 Mb) 的准确检测,整个流程仅需 8 小时。*Ion PGM 测序系统提供高通量灵活性,能够经济高效地筛查 2 - 24 份样品,尽量降低对批量样品的需求。

*ReproSeq PGS View 工作流程的周转时间低至 8 小时(搭配 Ion 314™ 芯片)以及低至 10 小时(搭配 Ion 318™ 芯片)。

经济高效的分析工作流程,样品通量具有灵活性
ReproSeq PGS View 试剂盒与 Ion PGM 系统为各种水平的样品通量提供可扩展性和灵活性。从精选的 3 个测序芯片中选择一个匹配您通量需求的芯片来处理 2 - 24 份样品,从而尽量降低对批量样品的需求,实现成本效益。

实现时间敏感型样品的快速周转
得益于快速高效的半导体测序与等温扩增模板制备技术,ReproSeq PGS View 试剂盒为非整倍性分析提供了快速台式 NGS 工作流程。从植入前胚胎中分离出单个细胞到非整倍性检测,这一工作流程只需 8 小时(筛查 2 份样品)或只需 10 小时(筛查 24 份样品)。这种快速周转时间有助于快速鉴定染色体正常胚胎以进行研究。

ReproSeq™ PGS 316 View 试剂盒(含芯片)包括用于全基因扩增、文库构建、模板制备以及使用 Ion PGM 系统测序的所有试剂,共可处理 120 份样品。试剂盒组分如下:

•Ion SingleSeq™ 试剂盒(24 次反应/试剂盒)—用于基因组 DNA 的提取、扩增和编码,只需不到 4 小时(包括手动操作时间)即可完成
• Ion PGM™ Template IA 500 试剂盒(A24622,8 次反应/试剂盒)—实现了更快的模板制备(2 小时,包括手动操作时间),由此通过非乳化、等温反应,将 DNA 克隆扩增到微珠表面
• Ion PGM™ Hi-Q™ View 测序试剂盒(A30044,8 次反应/试剂盒,200 bp 级)—可提供先进的测序化学且支持所有 200 bp 测序需求
• Ion PGM™ Wash 2 Bottle 套装 (A25591)—包含调理液和一个冲洗溶液 2 瓶套装,更换前可用于多达 40 次运行
• Ion 316™ Chip Kit v2 BC (4488149)—每个套装包含 8 个条形码芯片,与 Ion PGM 系统联用可进行样品追踪和测序运行。


• 该测序试剂盒应与 Ion OneTouch™ ES(微珠富集仪)和 Ion PGM™ 富集微珠 (4478525) 配合使用。
•该试剂盒仅与 Ion PGM 系统兼容。

仅供科研使用。不可用于诊断程序。
规格
适用于(应用)快速测序
适用于(设备)Ion PGM™ 系统
产品类型PGS 316 View 试剂盒(含芯片)
数量120 samples
运输条件经批准可置于湿冰或干冰上运输
通量120 个样品
产品线ReproSeq
原始材料DNA
Unit SizeEach
内容与储存
• Ion PGM Template IA 耗材 500(室温储存)
• Ion PGM Template IA 试剂 500(储存于 2–8℃)
• Ion PGM Template IA 反应 500(储存于 -5 至 -30℃)
• Ion PGM Template IA 溶液 500(室温储存)
• Ion SingleSeq 试剂盒(储存于 -5 至 -30℃)
• Ion PGM Seq 耗材(室温储存)
• Ion PGM Hi-Q View Seq 试剂(储存于 -5 至 -30℃)
• Ion PGM Hi-Q View Seq 溶液(室温储存)
• Ion PGM Hi-Q Seq dNTP 试剂盒(储存于 -5 至 -30℃)
• Ion PGM Wash 2 Bottle 套装(室温储存)
Ion 316™ Chip Kit v2 BC

常见问题解答 (FAQ)

What are the catalog numbers for the Ion ReproSeq PGS View Kits?

Ion ReproSeq PGS 314 View Kit, without chips (16 samples): Cat. No. A32251
Ion ReproSeq PGS 316 View Kit, without chips (120 samples): Cat. No. A32252
Ion ReproSeq PGS 318 View Kit, without chips (192 samples): Cat. No. A32253
Ion ReproSeq PGS 314 View Kit, with chips (Up to 2 samples per chip, 8 chips included): Cat. No. A32243
Ion ReproSeq PGS 316 View Kit, with chips (Up to 15 samples per chip, 8 chips included): Cat. No. A32249
Ion ReproSeq PGS 318 View Kit, with chips (Up to 24 samples per chip, 8 chips included): Cat. No. A32250

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is included in the Ion PGM Wash 2 Bottle Kit (Cat. No. A25591)?

Here are the kit components:

Wash 2 Bottle w/label (2 L): 1 bottle
Wash 2 Bottle Conditioning Solution: 125 mL

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is included in the Ion PGM Hi‑Q View Sequencing Kit (Cat. No. A30044)?

Here are the kit components:

Ion PGM Sequencing Supplies (Part No. A25587)
- Wash 1 Bottle w/label (250 mL): 1 bottle
- Wash 3 Bottle w/label (250 mL): 1 bottle
- Ion PGM Reagent Bottle Sipper Tubes (blue): 16 tubes
- Ion PGM Wash Bottle Sipper Tubes (gray): 8 tubes for 250-mL bottles; 4 tubes for 2-L bottles
- Reagent Bottles w/labels (50 mL): 25 bottles

Ion PGM Hi-Q View Sequencing Reagents (Part No. A30043)
- Ion PGM Hi-Q View Sequencing Polymerase (yellow cap): 36 µL
- Ion PGM Hi-Q View Sequencing Primer (white cap): 144 µL
- Ion PGM Hi-Q View Control Ion PGM Ion Sphere Particles (clear cap): 60 µL

Ion PGM Hi-Q View Sequencing Solutions (Part No. A30275)
- Ion PGM Hi-Q View Sequencing W2 Solution: 4 x 125 mL
- Ion Cleaning Tablet: 4 tablets
- Annealing Buffer: 12 mL
- Ion PGM Hi-Q View Sequencing W3 Solution: 2 x 100 mL

Ion PGM Hi-Q Sequencing dNTPs (Part No. A25590)
- Ion PGM Hi-Q Sequencing dGTP (black cap): 80 µL
- Ion PGM Hi-Q Sequencing dCTP (blue cap): 80 µL
- Ion PGM Hi-Q Sequencing dATP (green cap): 80 µL
- Ion PGM Hi-Q Sequencing dTTP (red cap): 80 µL

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What are the components of the Ion PGM Template IA 500 Kit (Cat. No. A24622)?

Here are the kit components:

Ion PGM Template IA Supplies 500 (Part No. A24618)
- Ion OneTouch ES Supplies: 1 bag

Ion PGM Template IA Reagents 500 (Part No. A24619)
- Ion PGM Template IA Pellets 500: 4 foil pouches [1]
- Ion PGM Template IA ISP Dilution Buffer (yellow cap): 1 mL
- Ion PGM Template IA Start Solution (purple cap): 2 x 1200 µL

Ion PGM Template IA Reactions 500 (Part No. A24620)
- Ion PGM Template IA Ion Sphere Particles (orange cap): 86 µL
- Ion PGM Template IA Primer Mix S (black cap): 48 µL
- Ion PGM Template IA Primer Mix L (blue cap): 48 µL [2]
- Ion PGM Template IA Rehydration Buffer (white cap): 2 x 1.5 mL

Ion PGM Template IA Solutions (Part No. A24621)
- Ion PGM Template IA Stop Solution: 2.8 mL
- Ion PGM Template IA Recovery Solution: 7 mL
- Ion PGM Template IA Wash Solution: 4 mL
- MyOne Beads Wash Solution (green cap): 1.04 mL
- Neutralization Solution (red cap): 40 µL
- Tween Solution: 3.5 mL

Note: [1] One Ion PGM Template IA Pellet 500 per tube per pouch.
[2] Primer Mix L is intended for amplification of long templates and is not used with the Ion ReproSeq PGS View Kit

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is included in the Ion ReproSeq PGS View Kits?

Here are the kit components:

- Ion SingleSeq Kit (1, 5 or 8 boxes per Ion ReproSeq PGS View Kit, depending on the kit)
- Ion PGM Template IA 500 Kit (4 boxes/set, 1 set per Ion ReproSeq PGS View Kit)
- Ion PGM Hi-Q View Sequencing Kit (4 boxes/set, 1 set per Ion ReproSeq PGS View Kit)
- Ion PGM Wash 2 Bottle Kit (1 box per Ion ReproSeq PGS View Kit)
- Ion 314, Ion 316, or Ion 318 Chip Kit v2 BC (8 pack; Cat. Nos. A32243, A32249, A32250 only)

Note: The Ion SingleSeq Kit provides 24 unique Barcode Adapters and reagents to prepare 24 barcoded Ion SingleSeq libraries. The Ion PGM Template IA 500 Kit provides reagents and supplies to perform 4 isothermal amplification reactions. The Ion PGM Hi-Q View Sequencing Kit and Ion PGM Wash 2 Bottle Kit provide reagents and materials to perform 4 initializations and up to 12 sequencing runs on the Ion PGM Sequencer. Store the kit components as directed. See the Ion PGM Hi‑Q View Sequencing Kit User Guide (http://tools.thermofisher.com/content/sfs/manuals/MAN0014583_IonPGM_HiQ_View_Sequenc_UG.pdf) for more information.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.