CultureOne™ 添加剂 (100X)
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CultureOne™ 添加剂 (100X)
Gibco™

CultureOne™ 添加剂 (100X)

CultureOne 添加剂是一种化学成分确定的无血清添加剂,可显著改善神经干细胞(neural stem cell,以下简称 NSC)向神经元的分化。传统的分化方法容易使神经干细胞过度生长,加重培养基负担,与之相比,CultureOne 添加剂可减少 75%了解更多信息
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货号数量
A33202015 mL
货号 A3320201
价格(CNY)
1,968.76
飞享价
Ends: 31-Dec-2025
2,564.00
共减 595.24 (23%)
Each
添加至购物车
数量:
5 mL
价格(CNY)
1,968.76
飞享价
Ends: 31-Dec-2025
2,564.00
共减 595.24 (23%)
Each
添加至购物车
CultureOne 添加剂是一种化学成分确定的无血清添加剂,可显著改善神经干细胞(neural stem cell,以下简称 NSC)向神经元的分化。传统的分化方法容易使神经干细胞过度生长,加重培养基负担,与之相比,CultureOne 添加剂可减少 75% 以上的神经前体细胞污染,较大限度减少细胞死亡,且对其他激酶介导的通路没有影响。这形成的神经元细胞分布均匀,分化完全,从而改善下游试验,加速神经元成熟,且能稳定维持培养体系 5 周或更长时间。

每瓶 CultureOne 添加剂含有 100 X 5-mL 液体试剂,可以轻松添加至常规神经元分化培养基中,如含 B-27 添加剂神经元基础培养基。使用 CultureOne 添加剂分化两周后,可以清除添加剂,并且得到的培养物将包含五周或更少的前体细胞。使用 CultureOne 添加剂分化两周后,可以更换不含该添加剂的培养基,此时培养物含有很少的前体细胞,持续 5 周或更长时间。

改善下游检测
由于培养物中存在过度生长的前体细胞,因此很难对 NSC- 源性神经元进行分析。这些侵袭性细胞可使成像和电生理学实验变得困难重重,并且使基因表达检测的生理相关性方面存在疑问。CultureOne 补充剂可以减少增殖前体细胞,使您获得更清洁且更相关的神经细胞群、从而显著改善测定体验和结果。CultureOne 添加剂可减少前体细胞增殖,为您提供更纯、更相关的神经元细胞群,显著改善检测过程和结果。

加速神经元成熟
与未处理的 NSC- 源性神经元培养物相比,加入 CultureOne 添加剂后,NSC- 源性神经元表达成熟生理标志物的速度更快,并且形成更长的神经突。CultureOne 添加剂处理的神经元还表达更多的电压门控钙离子通道,这是神经成熟和兴奋的重要标志物。

NSC- 源性神经元培养时间延长
对于需要 NSC-源性神经元长时间分化的实验来说,CultureOne 添加剂能够连续 5 周或更长时间维持富集、分化的神经元。相比之下,传统的分化方法会导致神经前体细胞增殖,其容易结团,引起培养物脱落。

关于建立的 CultureOne 添加剂使用方案,请访问我们的 Gibco 神经生物学方案手册

关于使用 Gibco 神经生物学产品的文献,请查看我们的 Gibco CellCite

规格
描述Neural Cells, Serum-Free
产品线CultureOne™
数量5 mL
运输条件干冰
经过测试内毒素,无菌
形式液体
产品类型Media Broth
Unit SizeEach
内容与储存
储存在冰箱(-5 至 -20°C)中并避光。

常见问题解答 (FAQ)

What are the storage conditions for CultureOne Supplement? Can I store it at 4 degrees C and for how long? Can I aliquot unused product and freeze it at -20 degrees C?

We recommend that you thaw the CultureOne Supplement and then aliquot it out for working stocks and re-freeze the aliquots. We have found that the product can undergo up to 3 freeze-thaws without loss of activity. CultureOne-supplemented media is good for 2 weeks stored at 4 degrees C in the dark.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I am using the B-27 Plus Neuronal Culture System. What do I do if my neuronal cells are not attaching or are clumped?

In primary neuronal cultures, this is typically a function of uneven coating or if neurons are plated at too high of a density. In NSC differentiation experiments, this could be due to overproliferation of neural progenitor cells. Differentiating with the B-27 Plus Neuronal Culture System and CultureOne Supplement can eliminate more than 75% of neural progenitor cells and the clumps they form. Please see the CultureOne Supplement User Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0016204_CultureOneSupplement_UG.pdf) for more information.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I am planning to use the B-27 Plus Neuronal Culture System. How can I control the number of glial cells in my neuronal culture?

Add CultureOne Supplement at day 0 with the B-27 Plus Neuronal Culture System to fully suppress both astrocytes and oligodendrocytes with no detrimental effect on neurons. Delaying the addition of CultureOne Supplement to later time points results in increasing levels of astrocytes. Refer to the CultureOne Supplement User Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0016204_CultureOneSupplement_UG.pdf) for more information.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What specific types of neurons will CultureOne Supplement work with?

CultureOne Supplement accelerates the maturation of all types of differentiating neurons.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I thaw CultureOne Supplement and re-freeze it?

CultureOne Supplement can be frozen and thawed up to 3 times without affecting the product performance.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.