What do I need to do to prep the barcode adapters in the IonCode Barcode Adapters 1-96 Kit (Cat. No. A29747)?
When handling barcode adapters, be careful to avoid cross-contamination by changing gloves frequently and by carefully removing the plate seal.
- Pulse vortex the IonCode Barcode Adapters plate several times.
- Centrifuge the IonCode Barcode Adapters plate for 1 minute at 1000 x g to collect contents.
- Carefully remove, then discard the plate seal.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
How many reactions are possible with the IonCode Barcode Adapters 1-96 Kit (Cat. No. A29747)?
The IonCode Barcode Adapters 1-96 Kit consists of 1 x 96-well plate, with 20 µL of each barcode per well. The plate consists of 960 reactions in total with 10 reactions for each of the 96 barcodes.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
In what format are the barcode adapters in the IonCode Barcode Adapters 1-96 Kit (Cat. No. A29747)?
The IonCode Barcode Adapters 1-96 Kit consists of 1 x 96-well plate, with 20 µL of each barcode per well. The barcodes are pre-diluted, ready-to-use. The 96-well plate format allows multichannel pipetting for more efficient work.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
What are the shipping and storage conditions for IonCode Barcode Adapters 1-96 Kit (Cat. No. A29747)?
The IonCode Barcode Adapters 1-96 Kit is shipped on dry ice and we recommend storing at -30 degrees C to -5 degrees C in an upright position.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
What is included in the Ion AmpliSeq Microbiome Health Research Kit, Ion 550 bundle?
The Ion AmpliSeq Microbiome Health Research Kit, Ion 550 bundle (Cat. No. A46497) consists of the Ion AmpliSeq Microbiome Health Research - 16S rRNA Gene Pool, the Ion AmpliSeq Microbiome Health Research - Target Species Pool, the Ion AmpliSeq Library Kit Plus, the IonCode Barcode Adapters, the Ion 550 Kit - Chef, and the Ion 550 Chip Kit for the rapid preparation of barcoded sample libraries from bacterial DNA. Sufficient reagents are provided to prepare uniquely barcoded DNA libraries from 384 research samples.
Note: The Ion Library TaqMan Quantitation Kit (Cat. No. 4468802) must be purchased separately.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.