Amplex™ Red 试剂
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Amplex™ Red 试剂
引用和文献 (15)
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Amplex™ Red 试剂

Amplex Red 试剂，是高度灵敏和稳定的 H2O2 探针，是过氧化物酶最好的荧光底物。 存在辣根过氧化物酶 (HRP) 时，Amplex Red了解更多信息
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货号数量染料类型
A360065 x 1 mgAmplex UltraRed Reagent
A122225 mgAmplex Red Reagent
A2217710 x 10 mgAmplex Red Reagent
货号 A36006
价格（CNY)
4,842.00
Each
添加至购物车
数量:
5 x 1 mg
染料类型:
Amplex UltraRed Reagent
价格（CNY)
4,842.00
Each
添加至购物车
Amplex Red 试剂，是高度灵敏和稳定的 H2O2 探针，是过氧化物酶最好的荧光底物。 存在辣根过氧化物酶 (HRP) 时，Amplex Red 试剂与 H2O2 以 1:1 的化学定量比反应，形成高荧光的试卤灵 (resorufin)。 因为很多不同的酶反应都会生成 H2O2，研究人员可以使用 Amplex Red 试剂检测各种酶的活性（Amplex Red 分析试剂盒）。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型Amplex UltraRed Reagent
形式Solid
数量5 x 1 mg
运输条件Room Temperature
底物HRP (Horseradish Peroxidase) Substrate
底物类型Chemical substrate
经证实的应用Cell Analysis
适用于（应用）Hydrogen Peroxidase Assay
适用于（设备）Fluorometer, Microplate Reader
产品线Amplex
产品类型Red Hydrogen Peroxide
Unit SizeEach
内容与储存
在冰箱（-5 至 -30°C）中储存。

常见问题解答 (FAQ)

I'm using an Amplex Red kit, the reagent changes color to pink almost immediately in my own Krebs-Ringer buffer but not in HBSS. Why is this?

The components of Krebs-Ringer buffer (salts) should not cause oxidation of the Amplex reagent (which, in the presence of peroxidase and H2O2 oxidizes to resorufin, which is pink in color and fluorescent). Try water alone (the water used to make the Krebs-Ringer buffer). Since Hank's Buffered Saline Solution is typically purchased rather than made in the lab, it likely would not have the same contaminant. Another option is to degas the buffer prior to use to removed dissolved oxygen radicals.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (15)

引用和文献
Abstract
A genetically encoded photosensitizer.
Authors:Bulina ME, Chudakov DM, Britanova OV, Yanushevich YG, Staroverov DB, Chepurnykh TV, Merzlyak EM, Shkrob MA, Lukyanov S, Lukyanov KA
Journal:Nat Biotechnol
PubMed ID:16369538
'Photosensitizers are chromophores that generate reactive oxygen species (ROS) upon light irradiation. They are used for inactivation of specific proteins by chromophore-assisted light inactivation (CALI) and for light-induced cell killing in photodynamic therapy. Here we report a genetically encoded photosensitizer, which we call KillerRed, developed from the hydrozoan chromoprotein anm2CP, ... More
Amplex UltraRed enhances the sensitivity of fluorimetric pyruvate detection.
Authors:Zhu A, Romero R, Petty HR,
Journal:Anal Biochem
PubMed ID:20382105
Pyruvate, which is produced in the final step of glycolysis, participates in anabolic metabolism, catabolic metabolism, and signal transduction. We have recently reported a new sensitive and selective fluorimetric assay for pyruvate measurement using Amplex Red as a fluorogenic dye. However, the fluorescence of the reaction product, resorufin, is pH ... More
Ultrahigh-throughput screening in drop-based microfluidics for directed evolution.
Authors:Agresti JJ, Antipov E, Abate AR, Ahn K, Rowat AC, Baret JC, Marquez M, Klibanov AM, Griffiths AD, Weitz DA,
Journal:Proc Natl Acad Sci U S A
PubMed ID:20142500
The explosive growth in our knowledge of genomes, proteomes, and metabolomes is driving ever-increasing fundamental understanding of the biochemistry of life, enabling qualitatively new studies of complex biological systems and their evolution. This knowledge also drives modern biotechnologies, such as molecular engineering and synthetic biology, which have enormous potential to ... More
Resveratrol promotes clearance of Alzheimer's disease amyloid-beta peptides.
Authors:Marambaud P, Zhao H, Davies P,
Journal:J Biol Chem
PubMed ID:16162502
Several epidemiological studies indicate that moderate consumption of wine is associated with a lower incidence of Alzheimer's disease. Wine is enriched in antioxidant compounds with potential neuroprotective activities. However, the exact molecular mechanisms involved in the beneficial effects of wine intake on the neurodegenerative process in Alzheimer's disease brain remain ... More
Facile SNP detection using bifunctional, cross-linking oligonucleotide probes.
Authors:Peng X, Greenberg MM,
Journal:Nucleic Acids Res
PubMed ID:18281702
A facile, sensitive method for detecting specific sequences of oligonucleotides was developed. Detection of DNA sequences with single nucleotide discrimination is achieved by combining the selectivity of hybridization with an efficient cross-linking reaction. Readily synthesized bifunctional oligonucleotide probes containing a modified pyrimidine that is capable of forming interstrand cross-links under ... More
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