配方50% suspension in PBS containing 0.02% Sodium azide
产品线Pierce™
产品类型亲和树脂
数量2 mL
运输条件湿冰
固定相抗-DYKDDDDK 单克隆抗体
基质Affinity Resin
Unit SizeEach
内容与储存
1 mL 沉淀树脂。2 mL,以 50% 浆液(溶于含 0.02% 叠氮化钠的 PBS)形式提供。2–8°C 储存。
常见问题解答 (FAQ)
What is the slurry/suspension percentage for your anti-DYKDDDDK ("FLAG") products?
Anti-DYKDDDDK Magnetic Agarose (Cat. Nos. A36797, A36798, A36799B) is offered as a 25% suspension (1 mL of 25% suspension = 0.25 mL of settled beads). UltraLink-based Anti-DYKDDDDK Affinity Resin (Cat. Nos, A36801, A36803, A36804) is offered as a 50% slurry (1 mL of 50% slurry = 0.5 mL of settled resin).
How do I cleave off the DYKDDDDK ("FLAG") tag after purification?
An enterokinase cleavage site behind the DYKDDDDK (FLAG) tag can allow complete removal of the DYKDDDDK (FLAG) tag leaving no additional amino acids. We offer EKMax Enterokinase (Cat. Nos. E18001 and E18002) that can be used for this purpose. Subsequently, the EKMax Enterokinase can be removed using EK-Away Resin (Cat. No. R18001), a resin conjugated with soybean trypsin inhibitor, which has high affinity for enterokinase.