Please consider the latest version of this product introducing the most recent chemistry and protocol improvements for best performance. For more information, visit A57283 product page
Ion Torrent™
Ion AmpliSeq™ HD 文库试剂盒
Ion AmpliSeq HD 文库试剂盒设计用于产生扩增子文库,作为 Ion AmpliSeq HD 测序工作流程的一部分。该试剂盒与使用 Ion了解更多信息
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货号
数量
A37694
24 Reactions
货号 A37694
价格(CNY)
-
数量:
24 Reactions
Ion AmpliSeq HD 文库试剂盒设计用于产生扩增子文库,作为 Ion AmpliSeq HD 测序工作流程的一部分。该试剂盒与使用 Ion AmpliSeq Designer 工具 (www.ampliseq.cn) 创建的定制检测组合兼容,并使用 Ion GeneStudio S5 系列仪器进行测序。Ion AmpliSeq HD 技术可实现从12通路到5000通路(在1池检测组合中为 1 x 5000-通路,或在2池检测组合中为 2 x 2500-通路)的可扩展聚合酶链反应 (PCR),用于独特分子标签 (UMT)−靶向测序,样品输入量低至 1 ng,检测水平低至 0.1%,具体取决于样品来源。
主要产品特点包括: •定制设计创建 •超高灵敏度检测 •双条形码样品倍增 •双向测序
定制设计创建 Ion AmpliSeq Designer 工具现在可用于创建与 Ion AmpliSeq HD 技术配合使用的定制设计。设计是按顺序进行,允许用户灵活创建区域设计、热点设计或融合设计。这些设计为单或双池设计,建议与从 cfDNA、cfRNA、cfTNA 或 FFPE 获得的样品配合使用。
超高灵敏度检测 Ion AmpliSeq HD 技术能够将超高检测水平降至 0.1%。对于具有已知变体的 cfDNA 对照,已使用 20 ng 的起始量观察到这些检测水平。其他样品类型(如 FFPE)的检测水平不同。
双条形码样品倍增 Ion AmpliSeq HD 文库试剂盒包括使用 Ion AmpliSeq HD 引物生成扩增子的试剂,这些引物用于制备用于测序的文库。使用 Ion AmpliSeq HD 双条形码试剂盒 1-24,在5’和3’端对文库进行双条形编码,该试剂盒可在单个芯片中同时检测多达24份样品。
双向测序 Ion AmpliSeq HD 文库试剂盒还提供来自两条链的测序信息。这样可以更好地纠正错误,并提高您的序列数据质量的可信度。
How long can 10X DNA working panel FWD and REV subpools be stored?
10X DNA working panel FWD and REV subpools can be stored at 4 degrees C for one week. For longer term storage, aliquot and store subpools at -20 degrees C.
Have there been any recent changes to the protocol for Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283)?
There have been some recent changes to the protocol. Please refer to the latest user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017392_Ion%20AmpliSeqHDLibraryKit_UG.pdf) for full details.
When should I use CRC versus HD Enhancer for Ion AmpliSeq HD library preparation?
CRC is a component of the Ion AmpliSeq HD Library Kit (Cat. No. A37694). CRC improves target amplification reactions for challenging panels that produce excessive amounts of primer dimer or panels that have ⋝500 primer pairs. We recommend using CRC with all panel types. HD Enhancer is a component of the Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283) and is also available separately as Cat. No. A53690. HD Enhancer is used for improved library quality and molecular coverage results. Guidelines for using HD Enhancer or CRC for Ion AmpliSeq HD library preparation are outlined in the user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017392_Ion%20AmpliSeqHDLibraryKit_UG.pdf).
Which barcodes are compatible with Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283)?
We recommend using the Ion AmpliSeq HD Dual Barcode Kit 1-24 (Cat. No. A37695) with Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283). The libraries are dual barcoded at the 5' and 3' ends and this enables the multiplexing of up to 24 samples in a single chip. Using these dual barcodes also reduces the risk of barcode cross-contamination.