Collibri™ 文库定量试剂盒
Collibri™ 文库定量试剂盒
Invitrogen™

Collibri™ 文库定量试剂盒

Invitrogen Collibri 文库定量试剂盒包括经过优化的可用于 Illumina NGS 文库定量测定的即用型预混液和文库稀释缓冲液。Collibri 文库定量预混液包括 Platinum II了解更多信息
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货号反应次数
A38524500500 Reactions
A38524100100 Reactions
货号 A38524500
价格（CNY)
6,964.00
Each
添加至购物车
反应次数:
500 Reactions
价格（CNY)
6,964.00
Each
添加至购物车
Invitrogen Collibri 文库定量试剂盒包括经过优化的可用于 Illumina NGS 文库定量测定的即用型预混液和文库稀释缓冲液。Collibri 文库定量预混液包括 Platinum II Taq 热启动 DNA 聚合酶，可方便准确地对 NGS 文库进行定量测定。预混液包含扩增反应所需的所有组分，包括引物和惰性参比染料，使用前无需任何制备操作。由于预混液中惰性参比染料具有通用浓度，该试剂盒无需调整即可与任何 qPCR 仪器配合使用。

Collibri 文库定量试剂盒的特点包括：
•对使用各种样品（包括降解样品）制备的文库进行准确定量测定
•易于使用，所有组分均为即用型—无需试剂制备操作
•视觉提示—包括用于追踪移液步骤的特殊配制染料
•Platinum 热启动技术—可提供出色的特异性、灵敏度，可在室温下进行反应构建，

具有无可比拟的便利性
Collibri 文库定量预混液含有 Platinum II Taq 热启动 DNA 聚合酶、经优化的缓冲液、dNTP、靶向接头序列的引物混合物、SYBR 绿色染料、惰性参比染料和可追踪移液步骤的蓝色染料。这种独特的配方在工作流程的每一步均易于使用：
•无需试剂制备操作
• 可追踪移液步骤
• 一种适用于各种长度和 GC/AT 含量水平的文库的循环方案
• 通用惰性参比染料浓度—适用于所有 qPCR 仪器的单一预混液

Collibri 文库定量试剂盒还包括一种即用型文库稀释缓冲液，其中含有稳定剂，以便在稀释后确保文库完整。缓冲液中含有可追踪移液步骤的黄色染料。当蓝色预混液和黄色稀释的文库混合时，变成绿色溶液。借助该特点，可简化将稀释后的文库或 DNA 标准品添加至 qPCR 板孔中的预混液的过程，有助于减少移液错误。

仅供科研使用。不可用于诊断程序。
规格
检测方法SYBR
适用于（应用）实时 PCR (qPCR)
适用于（设备）7500 Fast 系统、7900HT Fast 系统、QuantStudio™、ViiA™ 7 系统、StepOne™ 系统、StepOnePlus™ 系统
高 GC PCR 扩增效果低
反应次数500 Reactions
PCR 方法qPCR
聚合酶Platinum II Taq 热启动 DNA 聚合酶
产品线Collibri™
产品类型文库定量试剂盒
数量500 reactions
样品类型DNA, RNA
运输条件干冰
测试时间标准
产品规格试剂盒
Unit SizeEach
内容与储存
储存在 -20°C 下。

常见问题解答 (FAQ)

My qPCR instrument requires ROX. Which ROX dye do you recommend using with the Collibri Library Quantification Kit?

The Collibri Library Quantification Kit contains the Collibri Library Quantification Master Mix that already contains a universal concentration of ROX passive reference dye, so there is no need for additional adjustment with ROX dye regardless of the instrument you are working with.

Do I need to dilute the library prior to qPCR using the Collibri Library Quantification Kit?

Yes, the prepared library has to be diluted prior to running qPCR. Generally, it is recommended to run two dilution points, 1:10000 and 1:100000. For an established workflow where approximate library quantity is known, dilutions can be adjusted, but they need to fall within the range of the Collibri DNA Standards. Note that libraries should be diluted in the Collibri Library Dilution buffer that is included in the Collibri Library Quantification Kit. Dilution of the library stabilizes library molecules at low concentrations, producing accurate and reproducible results.

With the Collibri Library Quantification Kit, what effect do the visual cues for mixing have on library quantification results?

Colors in the Collibri Library Quantification Kit components have no effect on the universal passive reference dye, qPCR performance, or quantification results.

Can Collibri Library Quantification Kit be used with NGS libraries prepared for Ion Torrent sequencing?

No. However, the Collibri Library Quantification Kit contains the Collibri Library Quantification Master Mix that has premixed primers which are compatible with P5 and P7 adaptor sequences specific to the Illumina platform.