VetMAX™ 奶牛乳腺炎8重qPCR检测试剂盒
VetMAX™ 奶牛乳腺炎8重qPCR检测试剂盒
Applied Biosystems™

VetMAX™ 奶牛乳腺炎8重qPCR检测试剂盒

The VetMAX MastiType Myco8 Kit enables rapid, accurate detection of eight mastitis-causing pathogens in bovine milk. These eight pathogens, including了解更多信息
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货号数量
A39236100 tests
货号 A39236
价格(CNY)
-
数量:
100 tests
The VetMAX MastiType Myco8 Kit enables rapid, accurate detection of eight mastitis-causing pathogens in bovine milk. These eight pathogens, including six highly contagious and difficult to culture Mycoplasma strains, are detected using real-time PCR amplification of DNA unique to each pathogen in two separate reactions.

The advanced technology of the VetMAX MastiType real-time PCR assays offers these advantages:
• Designed for laboratories requiring high-throughput processing methods
• Faster results—typical time from sample to result is 2.5–3 hours, compared to up to 10 days for a culture
• High accuracy—high sensitivity and specificity for all individual pathogens
• Identifies and quantifies DNA, allowing detection of viable, dead, and growth-inhibited pathogens
• Allows use of preserved milk samples, eliminating the need for cooling during sample transportation
• Can be integrated into milk recording programs using preserved milk
• Validated with convenient MagMAX CORE nucleic acid purification method that does not require aspiration and centrifugation

The VetMAX MastiType Myco8 assay detects these eight pathogens in two separate PCR reactions:
Staphylococcus aureus
Streptococcus agalactiae
Mycoplasma bovis
Mycoplasma spp.
Mycoplasma alkalescens
Mycoplasma bovigenitalium
Mycoplasma californicum
Mycoplasma canadense

规格
适用于(设备)7500 Fast 系统、7500 系统、QuantStudio™
反应次数2 次反应
产品线VetMAX
产品类型应用于动物健康的 PCR 试剂和检测试剂盒
数量100 tests
样品类型DNA
运输条件干冰
检测方法引物-探针
高 GC PCR 扩增效果
PCR 方法多通路 qPCR
反应速度快速,标准
Unit SizeEach
内容与储存
• 2 x 1.2 mL 奶牛乳腺炎检测预混液。经优化的 PCR 缓冲液,含有热启动 DNA 聚合酶、镁离子和 dNTP 。
•1 x 440 μL 奶牛乳腺炎检测阳性对照。PCR 反应组分的阳性对照。
•1 x 550 μL 奶牛乳腺炎8重qPCR检测引物混合物 1。包含用于金黄色葡萄球菌、无乳链球菌、牛支原体和支原体属多重 PCR 扩增的引物,以及内部扩增对照 (IAC)。
•1 x 550 μL 奶牛乳腺炎8重qPCR检测引物混合物 2。包含用于产碱支原体、牛生殖道支原体、加利福尼亚支原体和加拿大支原体多重 PCR 扩增的引物以及内部扩增对照 (IAC)。

保存于 -5 至 -30°C 下。

常见问题解答 (FAQ)

What kind of milk samples can be tested with the VetMAX MastiType kits?

All VetMAX MastiType kits may be used with milk, bulk milk, frozen milk, or preserved milk (e.g., with bronopol).

What qPCR instrument can I use to run the VetMAX MastiType kits?

Either the Applied Biosystems 7500/7500 Fast Real-Time PCR System or the QuantStudio 5 Real-Time PCR System may be used to run the VetMAX MastiType kits.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Which sample prep kit do you recommend upstream of the VetMAX MastiType kits?

We recommend using the MagMAX CORE Nucleic Acid Purification Kit + MagMAX CORE Mastitis & Panbacteria Module (Cat. No. A40289) with the VetMAX MastiType kits.

Which organisms can be detected by each VetMAX MastiType kit?

The VetMax MastiType Micro4 Kit detects Mycoplasma bovis, Staphylococcus aureus, Streptococcus agalactiae, and Streptococcus uberis.

The VetMax MastiType Myco8 Kit detects Mycoplasma spp., Mycoplasma alkalescens, Mycoplasma bovis, Mycoplasma bovigenitalium, Mycoplasma canadense, Mycoplasma californicum, Staphylococcus aureus, and Streptococcus agalactiae.

The VetMax MastiType Multi Kit detects Staphylococcus aureus, Staphylococcus spp. (including all major coagulase-negative staphylococci), Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis, Escherichia coli, Enterococcus spp. (incl. E. faecalis and E. faecium), Klebsiella oxytoca and/or Klebsiella pneumoniae, Serratia marcescens, Corynebacterium bovis, Trueperella pyogenes and/or Peptoniphilus indolicus, Staphylococcal beta-lactamase gene (penicillin-resistance gene), Mycoplasma bovis, Mycoplasma spp., Yeast, and Prototheca spp.