MultiShot™ Mach1™ T1R Chemically Competent E. coli Cells, 96-well plate

Invitrogen MultiShot Mach1 T1^R Chemically Competent E. coli Cells are derived from a non-K-12, wild-type W E. coli strain (ATCC #9637) that was selected for its rapid growth rate and genetically manipulated to possess the most useful cloning strain features.

Features of MultiShot Mach1 T1^R competent cells include:

• Versatile strain for everyday cloning applications
• High quality plasmid preparations
• Useful for generating plasmid libraries
• Microtiter plate format design convenient for automated high-throughput cloning
• Competent cells pre-aliquoted into 96-well plate, ready for transformation

MultiShot Mach1 T1^R competent cells are fast growing chemically competent cells with a doubling time of approximately 50 minutes, with other popular strains averaging 70 minutes. Mach1 T1R colonies are clearly visible within eight hours of plating the transformation mix (ampicillin selection), enabling you to plate and pick colonies the same day. You can also prepare plasmid DNA four hours after inoculating a single, overnight-grown colony in the selective medium of choice (with any antibiotic). The Mach1 T1^R strain is useful for many cloning applications and provides transformation efficiencies of >1 x 10⁸ cfu/μg with plasmid DNA.

The Mach1 T1^R strain was engineered to carry the tonA mutation that confers resistance to T1 and T5 phage infection. The tonA genotype offers added security to valuable clones and libraries. The Mach1 T1^R strain also has the lacZΔM15 genotype, allowing blue-white screening on plates containing either X-Gal or Bluo-Gal. It also carries the recA1398 mutation that helps reduce the rate of recombination while propagating plasmid DNA, the endA1 mutation that increases plasmid DNA yield and quantity, and hsdR restriction system deletion to allow genomic DNA cloning.

MultiShot Mach1 T1^R competent cells offer:

• Transformation efficiencies of >1 x 10⁸ cfu/μg plasmid DNA
• tonA (fhuA) genotype to confer resistance to T1 and T5 phage
• lacZ∆M15 for blue-white color screening of recombinants
• hsdR mutation for efficient transformation of unmethylated DNA from PCR applications
• recA1398 mutation for reduced occurrence of homologous recombination in cloned DNA
• endA1 mutation for increased plasmid yield and quality