B-27™ Plus Neuronal Culture System with Neurobasal™ PhysioPlus Medium, reduced glucose

The B-27 Plus Neuronal Culture System combines B-27 Plus Supplement with the advanced Neurobasal PhysioPlus Medium to support healthy, functional neuronal cultures. Designed for primary and hPSC-derived neurons, the system allows a more physiologically relevant culture environment that promotes accelerated neuronal maturation, dependable neuronal activity, and sustained viability over extended culture periods. The system is suitable across neurobiology applications from functional assays including MEA and live-cell imaging to high-content imaging and high-throughput plate-reader assays.

Key Features & Benefits

Morphological and synaptic maturation

• Improves neurite outgrowth, synapse formation, neuronal morphology compared with conventional neuronal media approaches
• Accelerates neurite outgrowth and increases the number of synaptic-positive puncta

Supports earlier and sustained neuronal activity

Obtaining neurons that survive, mature, and exhibit dependable functional activity in vitro remains a major challenge in neuroscience research. The B-27 Plus Neuronal Culture System was developed to support neuronal activity that arises earlier in culture and remains stable across extended timepoints, helping researchers obtain results faster and perform longitudinal studies with greater confidence. Product performance has been verified by whole-cell patch clamp recordings and MEA experiments.

The system is optimized to support:

• Spontaneous neuronal activity
• Formation of functional neuronal networks
• Sustained electrophysiological activity over multiple weeks suitable for MEA workflows

More physiologically relevant neuronal culture conditions

Neurobasal PhysioPlus Medium is formulated to better reflect key aspects of the central nervous system (CNS) extracellular environment while maintaining robust neuronal health and performance.

• Osmolarity consistent with CNS extracellular conditions
• Reduced glucose concentrations, closer to physiological range
• Neuroactive components at relevant concentrations
• Verified electrophysiological properties of neurons in medium

Maintain healthy neurons longer in culture

The B-27 Plus Neuronal Culture System supports long-term neuron survival and health, enabling the highest survival rate of human stem cell-derived neurons and primary rodent neurons compared to other commercially available serum-free neuronal media systems. For example, 87% greater viability of rat cortical neurons was achieved with the B-27 Plus Neuronal Culture System compared to a leading competitor system, allowing your precious cell source to be seeded at lower density, seed more wells, and generate more data.

Researchers can culture neurons for extended periods to enable:

• Neuronal maturation studies
• Chronic treatment paradigms
• Disease modeling applications
• Longitudinal imaging experiments
• Repeated functional measurements

Multiple basal medium options available to meet your needs

Neurobasal PhysioPlus Medium - the recommended basal medium, formulated to better replicate the CNS extracellular environment, supporting sustained neuronal activity, improved viability, and enhanced maturity over extended culture periods.

Neurobasal PhysioPlus Medium without phenol red – for use during analysis of neurons in imaging-sensitive applications or where the presence of phenol red is undesired.

Neurobasal Plus Medium – an alternative basal medium with a low osmolality, similar to the classic Neurobasal Medium (Cat. No. 21103049)

Built on a trusted neuronal culture foundation

The original B-27 Plus Neuronal Culture System expanded upon the industry-standard Neurobasal and B-27 platform to provide enhanced neuronal robustness and culture performance. The addition of Neurobasal PhysioPlus Medium represents the next evolution of this system, helping researchers generate healthier, more functional, and more physiologically relevant neuronal cultures for advanced neuroscience research.