VetMAX™ MastiType Multi 套装试剂盒
VetMAX™ MastiType Multi 套装试剂盒
Applied Biosystems™

VetMAX™ MastiType Multi 套装试剂盒

This combo kit includes the VetMAX MastiType Multi Kit and the MagMAX CORE Nucleic Acid Purification Kit with Mastitis &了解更多信息
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货号数量
A40290100 tests
货号 A40290
价格(CNY)
-
数量:
100 tests
This combo kit includes the VetMAX MastiType Multi Kit and the MagMAX CORE Nucleic Acid Purification Kit with Mastitis & Panbacteria Module (also available separately).

VetMAX MastiType Multi Kit
The VetMAX MastiType Multi Kit enables rapid, accurate detection of 15 mastitis-causing pathogens and one penicillin-resistance gene in bovine milk. These 16 markers are detected using real-time PCR amplification of DNA unique to each pathogen in four separate reactions. The kit detects these mastitis-causing pathogens:
Staphylococcus aureus
Staphylococcus spp. (including all major coagulase-negative staphylococci)
Streptococcus agalactiae
Streptococcus dysgalactiae
Streptococcus uberis
Escherichia coli
Enterococcus spp. (including E. faecalis and E. faecium)
Klebsiella oxytoca (and/or K. pneumoniae)
Serratia marcescens
Corynebacterium bovis
Trueperella pyogenes and/or Peptoniphilus indolicus
• Staphylococcal β-lactamase gene (penicillin-resistance gene)
Mycoplasma bovis
Mycoplasma spp.
• Yeast
Prototheca spp.

Learn more about the VetMAX MastiType Multi Kit ›

For Veterinary Use Only. For In Vitro Use Only.

MagMAX CORE Nucleic Acid Purification Kit with Mastitis & Panbacteria Module
The MagMAX CORE sample extraction system is a universal magnetic bead-based separation system designed to meet current and future veterinary testing needs. It is compatible with both automated (using the KingFisher Flex Purification System) and manual purification methods. This modular technology is optimized for the purification of nucleic acids from a wide range of sample matrices commonly submitted for veterinary diagnostic testing.

The MagMAX CORE Mastitis & Panbacteria Module is offered here with the MagMAX CORE Nucleic Acid Purification Kit to aid in the processing of all bacteria, especially those important in the detection of mastitis in cattle and from challenging matrices like mastitic milk. We have included a specially optimized lysis solution, along with an adjusted extraction procedure to improve the recovery of nucleic acid from all bacteria, including gram-positive bacteria.

Learn more about the MagMAX CORE Nucleic Acid Purification Kit with Mastitis & Panbacteria Module ›

For Laboratory Use Including Veterinary and Environmental Uses.

规格
适用于(设备)7500 Fast 系统、7500 系统、QuantStudio™
测试数量100 tests
产品线VetMAX
产品类型应用于动物健康的 PCR 试剂和检测试剂盒
数量100 tests
样品类型DNA
运输条件干冰
检测方法引物-探针
PCR 方法多通路 qPCR
反应速度快速,标准
Unit SizeEach
内容与储存
• 1 x 50 mL MagMAX CORE 裂解液*
• 1 x 45 mL MagMAX CORE 结合溶液*
• 1 x 60 mL MagMAX CORE 洗涤液 1*
• 1 x 60 mL MagMAX CORE 洗涤液 2*
• 1 x 12 mL MagMAX CORE 洗脱缓冲液*
• 1 x 2.2 mL MagMAX CORE 磁珠*
• 1 x 1.25 mL MagMAX CORE 蛋白酶 K*
• 4 x 1375 μL MagMAX CORE 乳腺炎泛细菌**
• 4 x 1.2 mL MastiType 预混液**:含热启动 DNA 聚合酶,溶于含镁离子和 dNTP 的优化 PCR 缓冲液中
• 1 x 440 μL MastiType 阳性对照品**:PCR 反应组分的阳性对照品
• 1 x 550 μL MastiType 多引物混合物 1:包括金黄色葡萄球菌、牛结核分枝杆菌、肠球菌属(包括粪肠球菌和屎肠球菌)、牛棒状杆菌以及扩增内标 (IAC) 的多重 PCR 扩增的引物
• 1 x 550 μL MastiType 多引物混合物 2**:包含葡萄球菌 ß-内酰胺酶基因(青霉素抗性基因)、大肠杆菌、停乳链球菌、支原体菌属以及扩增内标 (IAC) 的多重 PCR 扩增的引物
• 1 x 550 μL MastiType 多引物混合物 3**:包含葡萄球菌属(包括所有主要 CNS)、无乳链球菌、乳房链球菌、原壁菌属的多重 PCR 扩增的引物
• 1 x 550 μL MastiType 多引物混合物 4**:包括酵母菌、粘质沙雷菌、产酸克雷伯氏菌和/或肺炎克雷伯菌、化脓菌和/或吲哚嗜胨菌的多重 PCR 扩增的引物

*储存在室温下。
**储存在 -20°C 下。

常见问题解答 (FAQ)

What kind of milk samples can be tested with the VetMAX MastiType kits?

All VetMAX MastiType kits may be used with milk, bulk milk, frozen milk, or preserved milk (e.g., with bronopol).

What qPCR instrument can I use to run the VetMAX MastiType kits?

Either the Applied Biosystems 7500/7500 Fast Real-Time PCR System or the QuantStudio 5 Real-Time PCR System may be used to run the VetMAX MastiType kits.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Which sample prep kit do you recommend upstream of the VetMAX MastiType kits?

We recommend using the MagMAX CORE Nucleic Acid Purification Kit + MagMAX CORE Mastitis & Panbacteria Module (Cat. No. A40289) with the VetMAX MastiType kits.

Which organisms can be detected by each VetMAX MastiType kit?

The VetMax MastiType Micro4 Kit detects Mycoplasma bovis, Staphylococcus aureus, Streptococcus agalactiae, and Streptococcus uberis.

The VetMax MastiType Myco8 Kit detects Mycoplasma spp., Mycoplasma alkalescens, Mycoplasma bovis, Mycoplasma bovigenitalium, Mycoplasma canadense, Mycoplasma californicum, Staphylococcus aureus, and Streptococcus agalactiae.

The VetMax MastiType Multi Kit detects Staphylococcus aureus, Staphylococcus spp. (including all major coagulase-negative staphylococci), Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis, Escherichia coli, Enterococcus spp. (incl. E. faecalis and E. faecium), Klebsiella oxytoca and/or Klebsiella pneumoniae, Serratia marcescens, Corynebacterium bovis, Trueperella pyogenes and/or Peptoniphilus indolicus, Staphylococcal beta-lactamase gene (penicillin-resistance gene), Mycoplasma bovis, Mycoplasma spp., Yeast, and Prototheca spp.