Oncomine Focus 检测,Chef-Ready 文库
Oncomine Focus 检测,Chef-Ready 文库
Ion Torrent™

Oncomine Focus 检测,Chef-Ready 文库

Oncomine Focus 检测是一种靶向下一代测序 (NGS)、多生物标志物检测,让您可以在单个工作流程中检测 DNA 和 RNA 的 52了解更多信息
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货号数量
A4200832 份样品
货号 A42008
价格(CNY)
-
数量:
32 份样品
Oncomine Focus 检测是一种靶向下一代测序 (NGS)、多生物标志物检测,让您可以在单个工作流程中检测 DNA 和 RNA 的 52 种癌症相关基因变体。其提供用于文库构建的试剂和两个用于从福尔马林固定石蜡包埋 (FFPE) 肿瘤样品制备扩增子文库的多重 PCR 引物的两个池 — 一个 DNA 池和一个 RNA 池。该检测仅需每个引物池中低至 10 ng 核酸,即可在单个工作流程中对热点、SNV、插入缺失、CNV 和基因融合进行分析。关于包括用于手动文库制备的试剂的试剂盒,请参见 Oncomine Focus 检测,AmpliSeq 文库

Oncomine Focus 检测,Chef-Ready 文库是用于 Ion Chef 系统和 Ion GeneStudio S5 系统的工作流程。它包含制备 32 份样品文库所需的所有试剂:

Oncomine Focus 检测,DNA Chef-Ready 检测板—包含 DNA 引物池
Oncomine Focus 检测,RNA Chef-Ready 检测板—包含 RNA 引物池
Ion AmpliSeq 试剂盒(用于 Chef DL8 试剂)—可使您在 Ion Chef 系统上进行自动文库制备

从样品到结果
Oncomine Focus 检测是 NGS 工作流程的一部分,有助于研究人员从数百个结果中过滤变体结果,以得到关键癌症驱动变体。 Oncomine生信数据分析结果 可过滤来自已上市药物信息、临床指南 (US-NCCN, US-FDA, EMSO, EMA) 和全球临床试验的注释,该过滤有助于您对结果进行背景分析和解读,以获得您所需的答案。了解更多关于 Oncomine Focus 检测Oncomine Oncology 的信息。

仅供科研使用。不可用于诊断程序。
规格
适用于(应用)定点突变
靶向测序库
样品数量32 samples
产品线Oncomine™
产品类型Focus Assay
数量32 份样品
运输条件经批准可在室温下或者湿冰或干冰上运输
工作流程步骤文库生成
Unit SizeEach
内容与储存
• 1 x Oncomine Focus 检测、DNA Chef-Ready 检测板 (2X)
• 1 x Oncomine Focus 检测、RNA Chef-Ready 检测板 (2X)
• 2 x Ion AmpliSeq 试剂盒(用于 Chef DL8 试剂)

注:不包括用于 cDNA 合成的 SuperScript 转录酶。必须单独销售。

常见问题解答 (FAQ)

With the Oncomine Focus Assay, Chef-Ready Library kit, where can I find the combined barcoded AmpliSeq libraries when the run is complete?

After the completion of a run, the Ion Chef Instrument holds the barcoded libraries in the tube loaded in Position D of the Reagents cartridge. To avoid fluid loss due to evaporation, remove and cap the tube of combined barcoded libraries as soon as possible after run completion. Do not leave the tube in the instrument longer than 24 hours after the start of the run. After 24 hours from the start of the run, the instrument chiller will stop actively cooling, and the sample will be held at 27 degrees C.

The tube in Position D of the Ion AmpliSeq Chef Reagents DL8 Cartridge will contain 700 µL of combined barcoded libraries at completion of the run. The libraries are at >>100 pM (total combined library concentration) and are ready to use in template preparation. One can store unused portions of combined libraries at 4 to 8 degrees C for up to 1 month. For longer-term storage, store at -30 to -10 degrees C.

With the Oncomine Focus Assay, Chef-Ready Library kit, can I process fewer than 8 samples per Ion AmpliSeq run on the Ion Chef System?

Yes, fewer than 8 samples may be processed per run but keep in mind that a run consumes kit reagents for 8 samples regardless of the sample number.

If I process less than 8 samples using the Oncomine Focus Assay, Chef-Ready Library kit, do I need to quantitate the final combined output library?

If you are processing ≤5 samples, we recommend that you quantify your output combined library by qPCR to ensure that an optimal concentration is used in templating reactions.

If you are processing fewer than 8 samples, it is preferable to add replicates or positive control samples to the run. Otherwise, pipet 15 µL of nuclease-free water as non-template control into column 1 wells that do not contain a DNA sample.

Where should I add the AmpliSeq primer pools on the Ion Chef Instrument deck?

The primer pools should be added to the Reagent Cartridge on the Ion Chef Instrument in positions A and B. Tubes in Positions C and D can be left empty. Uncap all 4 tubes in positions A, B, C, and D in the Ion AmpliSeq Chef Reagents DL8 cartridge.

What is the RNA input for the Oncomine Focus Assay, Chef-Ready Library kit?

Each reverse transcription reaction requires 10 ng of DNase-treated total RNA (≥1.43 ng/µL).