TMTpro™ 16plex 标签试剂组件
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TMTpro™ 16plex 标签试剂组件
引用和文献 (12)
Thermo Scientific™

TMTpro™ 16plex 标签试剂组件

Thermo Scientific TMTpro 16plex 标记试剂套件可实现通过串联质谱 (MS/MS)，对多达 16 份样品同时的进行蛋白鉴定和定量。该 TMTpro了解更多信息
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货号数量
A445201 x 5 mg（每个标签）
A445211 x 0.5 mg（每个标签）
A445226 x 0.5 mg（每个标签）
A5833496 次反应
货号 A44520
价格（CNY)
125,659.00
Each
添加至购物车
数量:
1 x 5 mg（每个标签）
价格（CNY)
125,659.00
Each
添加至购物车
Thermo Scientific TMTpro 16plex 标记试剂套件可实现通过串联质谱 (MS/MS)，对多达 16 份样品同时的进行蛋白鉴定和定量。
该 TMTpro 16plex 标记试剂套件的特点包括：
•多通路—对源自细胞、组织或生物体液的多达 16 份样品进行并行 MS 分析
• 稳健性—通路量增加使样品的缺失定量值减少，并使重复实验的可靠性提高
• 高效—胺反应性、NHS 酯活化试剂可确保高效标记所有肽，无需考虑蛋白序列或蛋白水解酶的特异性
• 兼容性—经过优化可与高分辨率 Thermo Scientific MS/MS 平台（如 Q Exactive 和 Orbitrap Fusion Tribrid 仪器系列，包括 Orbitrap Eclipse Tribrid 和 Orbitrap Exploris 480 质谱仪）配合使用，Proteome Discoverer 2.3 支持数据分析

TMTpro 标记试剂是新一代串联质谱标签，设计用于提高样品通量水平而不影响蛋白鉴定和定量的数量。TMTpro 标签结构与 TMT 标签结构类似，因为标签仍为同量异序和胺反应性，但是在长间隔区和异丁基脯氨酸质量报告基因区不同。在 MS/MS 片段化后，每个 TMTpro 标签在高分辨率 MS/MS 质谱的低质量区域生成一个唯一的报告基因质量（即 TMTpro 126-134Da），用于蛋白表达水平的相对定量。

TMTpro 标签的优势在于增加多通路相对定量、增加样品处理量、减少样品定量值的缺失。TMTpro 16plex 标记试剂适用于抑制剂剂量反应实验、时间进程实验、热转移测定或生物学重复等实验中多蛋白样品的分析。结合行业前列的高分辨率 Orbitrap 仪器和软件使用时，TMTpro 试剂可为定量蛋白表达分析提供综合全面的解决方案。

对于 TMTpro 标记肽进行高分辨率分析，Nanospray Flex 离子源推荐使用的 LC 色谱柱为 Acclaim PepMap 100 C18 LC 色谱柱（货号：164942 或 164939）。对于 EASY-Spray 离子源，推荐 LC 色谱柱为 EASY-Spray C18 LC 色谱柱（货号 ES802 或 ES803）。这些产品仅适用于应用范围有限的标签许可。

仅供科研使用。不可用于诊断程序。
规格
最终产品类型肽段
适用于（设备）质谱仪
标签或染料Tandem Mass Tag™ (TMT) 标记
数量1 x 5 mg（每个标签）
运输条件干冰
工作流程步骤蛋白质标记
检测方法质谱分析
产品线TMT
产品类型同量异位标记试剂套件
原始材料肽段
Unit SizeEach

常见问题解答 (FAQ)

What is the shelf life for the TMTpro 16plex Label Reagent Set?

The TMTpro 16plex Label Reagent Set is guaranteed for one year from the date of receipt in original, unopened packaging. This information can also be found in the Certificate of Analysis.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Can the TMT and TMTpro reagents be mixed?

No, the TMT and TMTpro reagents cannot be mixed because the reporter ions are in the same region.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Can TMTpro label reagents dissolved in anhydrous acetonitrile be dried down and stored for later use?

Yes, unused TMTpro label reagent that has been dissolved in anhydrous acetonitrile can be aliquoted into tubes, dried under vacuum at 25-30 degrees C, purged with nitrogen, and stored in a sealed bag with desiccant at -20 degrees C.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

What size formats is the TMTpro 16plex Label Reagent Set available in?

The TMTpro 16plex Label Reagent Set can be purchased in three different size formats:
1 x 0.5 mg (per tag), Cat No. A44521; sufficient for one 16plex isobaric experiment
6 x 0.5 mg (per tag), Cat No. A44522; sufficient for six 16plex isobaric experiments
1 x 5 mg (per tag), Cat No. A44520; sufficient for ten 16pex isobaric experiments

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

If I already have TMTpro 16-plex tags, what do I need to order to get a total of 18-plex TMTpro tags?

Depending upon the number of vials and amount of each tag in the vial for the TMTpro 16-plex tags you already have, you will have to purchase the appropriate TMTpro-134C and TMTpro-135N Label Reagents.
- If you already have TMTpro 16plex Label Reagent Set, 1 x 5 mg (Cat. No. A44520), you will have to purchase TMTpro-134C and TMTpro-135N Label Reagents, 1 x 5 mg (Cat. No. A52046).
- If you already have TMTpro 16plex Label Reagent Set, 6 x 0.5 mg (Cat. No. A44522), you will have to purchase TMTpro-134C and TMTpro-135N Label Reagents, 6 x 0.5 mg (Cat. No. A52048).

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

View all TMTpro™ 16plex 标签试剂组件 FAQs

引用和文献 (12)

引用和文献
Abstract
Automated 16-Plex Plasma Proteomics with Real-Time Search and Ion Mobility Mass Spectrometry Enables Large-Scale Profiling in Naked Mole-Rats and Mice.
Authors:Gaun A,Lewis Hardell KN,Olsson N,O'Brien JJ,Gollapudi S,Smith M,McAlister G,Huguet R,Keyser R,Buffenstein R,McAllister FE
Journal:Journal of proteome research
PubMed ID:33499602
Performing large-scale plasma proteome profiling is challenging due to limitations imposed by lengthy preparation and instrument time. We present a fully automated multiplexed proteome profiling platform (AutoMP3) using the Hamilton Vantage liquid handling robot capable of preparing hundreds to thousands of samples. To maximize protein depth in single-shot runs, we ... More
Discovering the Radiation Biomarkers in the Plasma of Total-Body Irradiated Leukemia Patients.
Authors:Gabriela R,Vera V,Pavel R,Helena R,Igor S,Marie D,Marketa M,Alena MF,Ales T
Journal:Radiation research
PubMed ID:38315067
The increased risk of acute large-scale radiological exposure for the world's population underlines the need for optimal radiation biomarkers. Ionizing radiation triggers a complex response by the genome, proteome, and metabolome, all of which have been reported as suitable indicators of radiation-induced damage in vivo. This study analyzed peripheral blood samples ... More
Improved Proteomics-Based Drug Mechanism-of-Action Studies Using 16-Plex Isobaric Mass Tags.
Authors:Zinn N,Werner T,Doce C,Mathieson T,Boecker C,Sweetman G,Fufezan C,Bantscheff M
Journal:Journal of proteome research
PubMed ID:33621079
Multiplexed quantitative proteomics enabled complex workflows to study the mechanisms by which small molecule drugs interact with the proteome such as thermal proteome profiling (TPP) or multiplexed proteome dynamics profiling (mPDP). TPP measures changes in protein thermal stability in response to drug treatment and thus informs on direct targets and ... More
Establishing Quality Control Procedures for Large-Scale Plasma Proteomics Analyses.
Authors:Patterson KL,Arul AB,Choi MJ,Oliver NC,Whitaker MD,Bodrick AC,Libby JB,Hansen S,Dumitrescu L,Gifford KA,Jefferson AL,Hohman TJ,Robinson RAS
Journal:Journal of the American Society for Mass Spectrometry
PubMed ID:37163770
Proteomics research has been transformed due to high-throughput liquid chromatography (LC-MS/MS) tandem mass spectrometry instruments combined with highly sophisticated automated sample preparation and multiplexing workflows. However, scaling proteomics experiments to large sample cohorts (hundreds to thousands) requires thoughtful quality control (QC) protocols. Robust QC protocols can help with reproducibility, quantitative ... More
Proteomic Profiling of Cerebrospinal Fluid by 16-Plex TMT-Based Mass Spectrometry.
Authors:Dey KK,Sun H,Wang Z,Niu M,Wang H,Jiao Y,Sun X,Li Y,Peng J
Journal:Methods in molecular biology (Clifton, N.J.)
PubMed ID:34905163
Mass spectrometry (MS) has become a mainstream platform for comprehensive profiling of proteome, especially with the improvement of multiplexed tandem mass tag labeling coupled with two-dimensional liquid chromatography and tandem mass spectrometry (TMT-LC/LC-MS/MS). Recently, we have established a robust method for direct profiling of undepleted cerebrospinal fluid (CSF) proteome with ... More
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