EasyPep™ MS 样品制备试剂盒
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EasyPep™ MS 样品制备试剂盒
引用和文献 (4)
Thermo Scientific™

EasyPep™ MS 样品制备试剂盒

使用经优化的 EasyPep MS 样品制备试剂盒在不到四小时内生成用于 MS 分析的优质蛋白组学样品。
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货号描述
A45733MS 样品制备试剂盒
A40006微型 MS 样品制备试剂盒
A57864Micro MS Sample Prep Kit
A45734大型样品制备试剂盒
A45735裂解缓冲液
A57865Peptide Clean-up Plate
货号 A45733
价格（CNY)
20,137.00
Online Exclusive
Ends: 31-Dec-2025
30,980.00
共减 10,843.00 (35%)
Each
添加至购物车
描述:
MS 样品制备试剂盒
价格（CNY)
20,137.00
Online Exclusive
Ends: 31-Dec-2025
30,980.00
共减 10,843.00 (35%)
Each
添加至购物车

EasyPep Mini MS 样品制备试剂盒可对培养的哺乳动物细胞和组织进行高效、可重现的处理以便进行蛋白组学 MS 分析。该试剂盒包含预配制的缓冲液、MS 级酶混合物、肽纯化柱和优化的方案，可在 3 小时内生成质谱兼容肽样品。试剂盒经过优化，可处理 10–100 μg 的蛋白样品，产生大量的 MS 即用型肽。

• 全面—包含用于酶切裂解的预配制试剂、肽纯化柱和用于多达 20 份样品的优化方案
• 经过优化—简化方案和试剂，尽可能减少处理样品所需的步骤和时间
• 灵活—试剂和方案已通过 10-100 µg 样品量的细胞、血浆和组织样品进行验证
• 节省时间—样品处理时间从一天以上缩短至不到三小时
• 兼容—最终制剂可用于直接 MS 分析和其他下游应用，包括 TMT 标记

用于 MS 分析的肽样品制备较复杂，步骤繁多，方案多变，导致肽的质量和数量都存在差异。本试剂盒设计用于提高重现性，同时节省手动操作和处理时间。通过添加通用核酸酶以降低来自核酸的粘度（替代超声处理）、用于半胱氨酸修饰的快速“一锅法”还原/烷基化溶液（脲甲基化，+ 57.02）和用于更完全酶切的胰蛋白酶/Lys-C 蛋白酶混合物，减少了步骤数量和处理时间。此外，该试剂盒还包括肽纯化柱和缓冲液，用于制备不含去污剂的肽样品进行 MS 分析。优化的试剂和方案可以产生兼容 TMT 标记和其他下游应用，或即时用于 MS 分析的高质量肽。

For Research Use Only. Not for use in diagnostic procedures.
规格
描述MS 样品制备试剂盒
最终产品类型肽
适用于（设备）质谱仪
标签或染料未标记
数量96 次反应
运输条件湿冰
工作流程步骤蛋白质消化
检测方法质谱分析
产品规格试剂盒
产品线EasyPep
产品类型样品制备试剂盒
原始材料蛋白样品
Unit SizeEach
内容与储存
  • 裂解液，25 mL，在 4°C 下储存
  • 通用核酸酶，25 kU，在 -20°C 下储存
  • 还原溶液，7 mL，在 4°C 下储存
  • 烷基化溶液，7 mL，在 4°C 下储存
  • 酶复溶液，7 mL，在 4°C 下储存
  • Pierce 胰蛋白酶/Lys-C 蛋白酶混合物，MS 级，5 x 100 µg，在 -20°C 下储存
  • 酶切终止液，7 mL，在 4°C 下储存
  • 肽纯化板，每套一件，在 4°C 下储存
  • 洗涤和洗脱板，每套一件，在 4°C 下储存
  • 样品制备板，每套一件，在 4°C 下储存
  • 洗涤液 A，40 mL，在 4°C 下储存
  • 洗涤液 B，3 x 27 mL，在 4°C 下储存
  • 洗脱溶液，2 x 20 mL，在 4°C 下储存

常见问题解答 (FAQ)

Are the EasyPep MS Sample Prep Kits compatible with vacuum pumps/dry-down devices?

As per protocol, peptide samples need to be dried down prior to LC-MS analysis. This step can be performed using vacuum pumps/dry-down devices without any harm caused to the device by the components present in the elution buffer within the EasyPep MS Sample Prep Kits.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

I have a lot of plasma samples to prepare for mass spectrometry analysis. Is there a way to perform abundant protein depletion in a 96-well plate?

The bulk Top14 depletion resin is compatible with a 96-well filter plate such as the Agilent filter microplate (Cat. No. 200957-100). Add 600 µL of resin slurry (50%) to each of the wells in the 96-well filter plate, add 10-20 µL of sample, and incubate with gentle shaking. Depleted samples can be collected in a new 96-well polypropylene plate by centrifugation for 2 mins at 1,000 x g or by using a vacuum manifold.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

After cell lysis, my mass spectrometry sample is very viscous and difficult to pipette. How do I reduce the sample viscosity?

High sample viscosity after lysis is due to release of DNA from the nucleus. Sonication or addition of a nuclease such as the Pierce Universal Nuclease (Cat. No. 88700, 88701, or 88702) can be used to degrade DNA and reduce sample viscosity.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

How do I process yeast samples with EasyPep Mini MS Sample prep Kit?

Add 0.5 mm diameter glass beads to the sample pelleted in Easy Pep Lysis Solution and proceed as recommended in the Easy Pep sample prep protocol. Vortex vigorously for few minutes, spin at 16,000 x g for 5 mins, and extract the supernatant for protein assay and further downstream sample preparation.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

How do I process bacterial samples with EasyPep MS Sample Prep kits?

Add 1-2 µL of 50 mg/mL Lysozyme Solution (Cat. No. 90082) to 200 µL of the EasyPep lysis buffer provided in the kit and proceed with the sample prep. Add 200 µL of the prepared lysis solution to 5 X 10E8 bacterial cells (E. coli cells) (OD600 equal to 1, corresponding to 1 X 10E8 CFUs). Lyse by pipetting the sample repeatedly and centrifuge at 16,000 x g for 5 mins. Alternatively, E. coli cells can be lysed in lysis buffer using bead beating or sonication instead of lysozyme. After lysis, dilute samples to 1 mg/mL using lysis buffer, proceed with the EasyPep protocol for reduction, alkylation, digestion, and clean up as described in the product manual.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

View all EasyPep™ MS 样品制备试剂盒 FAQs

引用和文献 (4)

引用和文献
Abstract
Discovery of Orally Available and Brain Penetrant AEP Inhibitors.
Authors:Krummenacher D,He W,Kuhn B,Schnider C,Beurier A,Brom V,Sivasothy T,Marty C,Tosstorff A,Hewings DS,Mesch S,Pinard E,Brändlin M,Hochstrasser R,Westwood P,Rothe J,Kronenberger A,Morandi F,Gutbier S,Schuler A,Heer D,Gloria LE,Joedicke L,Rudolph MG,Müller L,Grüninger F,Baumann K,Kaniyappan S,Manevski N,Bartels B
Journal:Journal of medicinal chemistry
PubMed ID:38090813
Alzheimer's Disease (AD) is the most widespread form of dementia, with one of the pathological hallmarks being the formation of neurofibrillary tangles (NFTs). These tangles consist of phosphorylated Tau fragments. Asparagine endopeptidase (AEP) is a key Tau cleaving enzyme that generates aggregation-prone Tau fragments. Inhibition of AEP to reduce the ... More
Development of a First-in-Class RIPK1 Degrader to Enhance Antitumor Immunity.
Authors:Wang J,Lu D,Yu X,Qi X,Lin H,Holloman BL,Jin F,Xu L,Ding L,Peng W,Wang M,Chen X
Journal:Research square
PubMed ID:38659866
The scaffolding function of receptor interacting protein kinase 1 (RIPK1) confers intrinsic and extrinsic resistance to immune checkpoint blockades (ICBs) and has emerged as a promising target for improving cancer immunotherapies. To address the challenge posed by a poorly defined binding pocket within the intermediate domain, we harnessed proteolysis targeting ... More
Proteomic Characterization of Transfusable Blood Components: Fresh Frozen Plasma, Cryoprecipitate, and Derived Extracellular Vesicles via Data-Independent Mass Spectrometry.
Authors:Hwang JH,Lai A,Tung JP,Harkin DG,Flower RL,Pecheniuk NM
Journal:Journal of proteome research
PubMed ID:39254217
Extracellular vesicles (EVs) are a heterogeneous collection of particles that play a crucial role in cell-to-cell communication, primarily due to their ability to transport molecules, such as proteins. Thus, profiling EV-associated proteins offers insight into their biological effects. EVs can be isolated from various biological fluids, including donor blood components ... More
Targeted sulfur(VI) fluoride exchange-mediated covalent modification of a tyrosine residue in the catalytic pocket of tyrosyl-DNA phosphodiesterase 1.
Authors:Zhao XZ,Barakat IA,Lountos GT,Wang W,Agama K,Mahmud MRA,Suazo KF,Andresson T,Pommier Y,Burke TR Jr
Journal:Communications chemistry
PubMed ID:39284936
Developing effective inhibitors of the DNA repair enzyme tyrosyl-DNA phosphodiesterase 1 (TDP1) has been challenging because of the enzyme shallow catalytic pocket and non-specific substrate binding interactions. Recently, we discovered a quinolone-binding hot spot in TDP1's active site proximal to the evolutionary conserved Y204 and F259 residues that position DNA. ... More