Oncomine™ 髓系疾病 RNA 测定试剂盒 GX v2

Ion Torrent Oncomine 髓系疾病 RNA 测定试剂盒 GX v2 是一种全面靶向下一代测序 (NGS)了解更多信息

货号	数量
A50754	1 个试剂盒

货号 A50754

价格（CNY)

1 个试剂盒

Ion Torrent Oncomine 髓系疾病 RNA 测定试剂盒 GX v2 是一种全面靶向下一代测序 (NGS) 试剂盒，设计用于对血液和骨髓样品中的髓系疾病相关 RNA 融合转录本进行灵敏检测。该试剂盒是 Oncomine 髓系疾病测定试剂盒 GX v2 （货号 A50694，包括 DNA 和 RNA 测定）的 RNA 测定组成部分。该测定试剂盒兼容 Genexus 集成测序仪，该测序仪可在样品到结果的自动化工作流程中执行文库制备、测序、分析和报告。根据不同的工作流程，最快只需一天即可获得结果。

Oncomine 髓系疾病 RNA 测定试剂盒 GX v2 的功能包括：
•可全面覆盖与髓系疾病相关的 >700 个关键融合转录本
•Genexus 测序仪上的自动化工作流程在一天之内即可由样品生成报告
•单次运行可在 GX5 芯片的每条泳道上对多达 32 份 RNA 样品进行测序
•手动操作时间少于 15 分钟

Oncomine 髓系疾病 RNA 测定试剂盒 GX v2 设计用于支持对髓系癌症进行研究。RNA 检测组合涵盖 30 种融合驱动基因，涵盖与主要髓系疾病相关的高度相关靶标，包括急性髓系白血病 (AML)、骨髓发育不良综合征 (MDS)、骨髓增生性肿瘤 (MPN)、慢性髓系白血病 (CML)、慢性粒单核细胞白血病 (CMML) 和幼年型粒单核细胞白血病 (JMML)。

多重引物设计利用 Ion AmpliSeq 技术在单次运行中可生成多个样品的结果。测序结果由 Genexus 软件使用优化的针对特定测定的分析工作流程进行自动分析。

该测定试剂盒包括一个 Ion AmpliSeq 寡核苷酸引物池和 Genexus 联管 1 & 2-AS 文库试剂，足以在 Genexus 集成测序仪上对多达 32 份 RNA 样品进行测序。一次端到端测序运行使用 GX5 芯片上的一条泳道，可容纳多达 32 份样品。额外的 Genexus 测序仪试剂和耗材必须单独订购。

适用于（应用）测序

适用于（设备）Genexus™ 集成测序仪

反应次数32

数量1 个试剂盒

样品类型来自血液或骨髓的 RNA

Unit SizeEach

常见问题解答 (FAQ)

What do I need to do to prep Genexus Templating Strips 3B-GX5 and 4-GX5 (Cat. No. A49782) for use on the Genexus platform?

Remove the templating strips from their boxes in the refrigerator or freezer and get them ready them for loading in the sequencer by equilibrating to/thawing at room temperature for 30 mins. Confirm that the strip contents are completely thawed before loading in the sequencer. If you are delayed in loading, keep the thawed strips on ice or at 4 degrees C until you load them in the sequencer.

Genexus Strip 3B‑GX5 contain magnetic beads in one or two positions, yellow or brown in color, that sometimes get trapped in the upper “keyhole” of the tube. Dislodge these beads from the keyhole before installing the strip in the sequencer using the following procedure for each strip:
- Invert the strip 3-4 times to dislodge beads that are trapped in the keyholes.
- To remove any remaining beads and liquid from the keyholes, grasp the strip at one end with the strip seal facing up, then swing the strip with a rapid, downward centrifugal arm motion, ending with a sharp wrist-flick.
- Grasp the strip at the other end, then repeat the centrifugal motion.
- Check tube positions for significant amounts of beads that are still trapped in keyholes and then repeat the centrifugal motion, if needed. It is acceptable if a few beads remain in the keyhole or on the tube wall, but most should be either in suspension or in a pellet at the bottom of the tube.

What is the difference between Genexus Templating Strips 3B-GX5 and 4-GX5 (Cat. No. A49782) and Genexus Templating Strips 3-GX5 and 4 (Cat. No. A40263)?

Genexus Templating Strips 3B-GX5 and 4-GX5 (Cat. No. A49782) has new Genexus Strip 3B that has the chemistry modified to improve templating and sequencing of GC rich regions.

What application is Genexus Templating Strips 3B-GX5 and 4-GX5 (Cat. No. A49782) compatible with?

Genexus Templating Strips 3B-GX5 and 4-GX5 is specifically designed for use with the Oncomine Myeloid GX v2 assay. For all other Genexus assays, please use Genexus Templating Strips 3-GX5 and 4 (Cat. No. A40263).

What are the components of Genexus Templating Strips 3B-GX5 and 4-GX5 (Cat. No. A49782)?

Genexus Templating Strips 3B-GX5 and 4-GX5 provides 8 pairs of strips per kit - Genexus Templating Strips 3B-GX5 (Part No. A47712) (Orange, 8 strips) and Genexus Templating Strips 4-GX5 (Part No. A46816) (Yellow, 8 strips). Each pair of strips is required to perform automated template preparation and chip loading of two GX5 chips using the Genexus Integrated Sequencer.

What are the shipping and storage conditions for Genexus Templating Strips 3B-GX5 and 4-GX5 (Cat. No. A49782)?

Genexus Templating Strips 3B-GX5 (Part No. A47712) (Orange, 8 strips) ships on a gel pack and should be stored at 2 to 8 degrees C. Genexus Templating Strips 4-GX5 (Part No. A46816) (Yellow, 8 strips) ships on dry ice and should be stored at -30 to -10 degrees C.