Are the components in the AAV-MAX Helper-Free AAV Production System available separately, or are they only sold as a kit?
The Viral Production Medium, Viral Production Cells 2.0, AAV-MAX Transfection Kit, Viral-Plex Complexation Buffer, and AAV-MAX Lysis Buffer are sold individually and here are the stand-alone catalog numbers:
- Viral Production Medium: Cat. No. A4817901, A4817902, A4817903
- Viral Production Cells 2.0: Cat. No. A49784, A51218
- AAV-MAX Transfection Kit: Cat. No. A50515, A50516
- Viral-Plex Complexation Buffer: A4983901
- AAV-MAX Lysis Buffer: A50520
Find additional tips, troubleshooting help, and resources within our Transfection Support Center.
Can I use other transfection reagents, such as PEI (polyethylenimine), in the AAV-MAX Helper-Free AAV Production System?
The AAV-MAX Transfection Reagent, AAV-MAX Tranfection Booster, and AAV-MAX Enhancer are specifically designed to work synergistically with the Viral Production Medium and Viral Production Cells 2.0 to provide maximum titers. Mixing and matching of reagents is not recommended, as this will decrease the overall performance.
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How scalable is the AAV-MAX transfection protocol?
The AAV-MAX transfection protocol is scalable from 24 deep-well plates to shake flasks to bioreactors. The user guide provides guidance on this.
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Are the LV-MAX Enhancer and AAV-MAX Enhancer different?
Yes, these are different, and each is optimized for its intended use.
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Will I see a drop in adeno-associated virus (AAV) titer if I don't use the AAV-MAX Enhancer with the AAV-MAX Helper-Free AAV Production System?
The AAV-MAX Enhancer is important for boosting AAV production. If the enhancer is not used, it will reduce system performance by 50% or more.
Find additional tips, troubleshooting help, and resources within our Transfection Support Center.