AAV-MAX 无辅助病毒 AAV 生产系统试剂盒
AAV-MAX 无辅助病毒 AAV 生产系统试剂盒
Gibco™

AAV-MAX 无辅助病毒 AAV 生产系统试剂盒

Gibco AAV-MAX 无辅助病毒 AAV 生产系统是一个可扩展、高滴度的平台,适用于悬浮 AAV 生产。其基于病毒生产培养基中克隆性 293F了解更多信息
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货号数量
A51217Sufficient for 1 L of Culture
货号 A51217
价格(CNY)
-
数量:
Sufficient for 1 L of Culture
Gibco AAV-MAX 无辅助病毒 AAV 生产系统是一个可扩展、高滴度的平台,适用于悬浮 AAV 生产。其基于病毒生产培养基中克隆性 293F 来源的病毒生产细胞 2.0 的高密度悬浮培养物。通过我们先进的脂质纳米颗粒转染试剂结合一种新型的生产增强剂,调控实现较优的病毒滴度。这些组分设计用于协同配合,实现较优滴度,无需优化试剂和方案。AAV-MAX 无辅助病毒 AAV 生产系统的特点包括:
• 组分协同配合,可产生高 AAV 滴度(大于 5 x 1010 vg/mL)(未纯化)
• 基于悬浮培养的系统具有从摇瓶到生物反应器规模的可扩展方案
• 简化工作流程 — 使用无辅助病毒三重转染的简化方案
• 克隆性 293F 来源的高产细胞系
• 稳健的转染方案适用于大规模转染 AAV-MAX 系统试剂盒中包括的组分有:
• 1 瓶病毒生产细胞 2.0
• 1 L 病毒生产培养基
• 1 个 AAV-MAX 转染试剂盒,用于 1 L 生产
• 100 mL Viral-Plex 络合缓冲液
• 100 mL AAV-MAX 裂解缓冲液
• 100 mL GlutaMAX 补充剂 所有组分也可单独购买。
仅供科研使用。不可用于诊断程序。
规格
细胞系病毒生产细胞 2.0
细胞类型肾脏(胚胎)
适用于(应用)AAV 生产
产品线AAV-MAX
产品类型AAV 生产系统
数量Sufficient for 1 L of Culture
有效期自生产之日起 12 个月
培养类型悬浮液细胞培养
无菌无菌
Unit SizeEach
内容与储存
• 1 瓶病毒生产细胞 2.0
• 1 L 病毒生产培养基
• 1 个 AAV-MAX 转染试剂盒,用于 1 L 生产
• 100 mL Viral-Plex 络合缓冲液
• 100 mL AAV-MAX 裂解缓冲液
• 100 mL GlutaMAX 补充剂

储存条件不同

常见问题解答 (FAQ)

Are the components in the AAV-MAX Helper-Free AAV Production System available separately, or are they only sold as a kit?

The Viral Production Medium, Viral Production Cells 2.0, AAV-MAX Transfection Kit, Viral-Plex Complexation Buffer, and AAV-MAX Lysis Buffer are sold individually and here are the stand-alone catalog numbers:
- Viral Production Medium: Cat. No. A4817901, A4817902, A4817903
- Viral Production Cells 2.0: Cat. No. A49784, A51218
- AAV-MAX Transfection Kit: Cat. No. A50515, A50516
- Viral-Plex Complexation Buffer: A4983901
- AAV-MAX Lysis Buffer: A50520

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I use other transfection reagents, such as PEI (polyethylenimine), in the AAV-MAX Helper-Free AAV Production System?

The AAV-MAX Transfection Reagent, AAV-MAX Tranfection Booster, and AAV-MAX Enhancer are specifically designed to work synergistically with the Viral Production Medium and Viral Production Cells 2.0 to provide maximum titers. Mixing and matching of reagents is not recommended, as this will decrease the overall performance.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

How scalable is the AAV-MAX transfection protocol?

The AAV-MAX transfection protocol is scalable from 24 deep-well plates to shake flasks to bioreactors. The user guide provides guidance on this.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Are the LV-MAX Enhancer and AAV-MAX Enhancer different?

Yes, these are different, and each is optimized for its intended use.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Will I see a drop in adeno-associated virus (AAV) titer if I don't use the AAV-MAX Enhancer with the AAV-MAX Helper-Free AAV Production System?

The AAV-MAX Enhancer is important for boosting AAV production. If the enhancer is not used, it will reduce system performance by 50% or more.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.