Efficient-Pro™ AGT™ 补料 1
Efficient-Pro™ AGT™ 补料 1
Gibco™

Efficient-Pro™ AGT™ 补料 1

Efficient-Pro AGT 补料 1 是用于生产单克隆抗体 (mAb) 的下一代中国仓鼠卵巢 (CHO) 补料,与之前的 Gibco了解更多信息
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货号数量
A52091011 L
A520910210 L
A5209103100 L
货号 A5209101
价格(CNY)
5,323.00
Each
添加至购物车
数量:
1 L
价格(CNY)
5,323.00
Each
添加至购物车
Efficient-Pro AGT 补料 1 是用于生产单克隆抗体 (mAb) 的下一代中国仓鼠卵巢 (CHO) 补料,与之前的 Gibco 和竞争产品相比,性能和易用性有了显著改善。Efficient-Pro AGT 补料 1 设计为配合 Efficient-Pro AGT 培养基使用时效果较好,已专门配制用于 CHO-K1 细胞系。Efficient-Pro AGT 系统可提高滴度和单位产量,改善蛋白质量和代谢物特征,与其他 CHO 培养基和补料产品相比,更易于使用,溶解度问题更少。

Efficient Pro AGT 系统将帮助您:
•使用带有 Gibco 商标的专利 Advanced Granulation Technology (AGT) 提高易用性,实现自动 pH 值和简化溶解
• 维持细胞活力以延长生物反应器运行时间,并尽可能提高滴度
• 改善下游输出,如蛋白糖基化、电荷变体和聚集
• 加快工艺开发并简化向生产规模的转移
• 通过一系列规格和包装配置提高灵活性
• 通过简化的适应方案降低细胞休克

Efficient-Pro AGT 补料 1 已配制为传统批次和流加生物工艺工作流程中(特别是与 CHO 细胞系变体结合时)mAb 和其他治疗药物的生物生产中使用的化学成分明确的非动物源性细胞培养补料。

Efficient-Pro AGT 补料 1 是一种均衡配方,其设计符合 mAb 生产的代谢需求,且不含生长因子或胰岛素,以确保批间一致性和生产效率。证明与处于行业前列的培养基和补料系统相比,Efficient-Pro AGT 系统配合 Efficient-Pro AGT 培养基使用时,可提高 CHO-K1 细胞系中的生产率,有助于加速上市时间并使您获得成功。

仅供研究使用。不可用于诊断程序。
规格
细胞系CHO-DG44、CHO-S、CHO-K1、GS-CHO
培养类型哺乳动物细胞培养
环保功能绿色可持续包装
数量1 L
运输条件室温
分类非动物源性, 化学成分明确, 无蛋白, 无血清
形式AGT 干粉剂型
Unit SizeEach

常见问题解答 (FAQ)

Can I use Efficient-Pro AGT Feed 1 with other cell lines besides CHO-S and DG-44 cell line variants?

Efficient-Pro AGT Feed 1 has been designed specifically for CHO-S and DG-44 cells; however, it has been demonstrated to improve performance in other CHO variants and even other parental cell lines.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I currently supplement my CHO cells with insulin and other growth factors. Should I continue the use of these other additives when using Efficient-Pro AGT Feed 1?

We recommend continuing current supplementation protocols including insulin and other growth factors when using Efficient-Pro AGT Feed 1.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the shelf-life of Efficient-Pro AGT Feed 1?

The shelf-life of Efficient-Pro AGT Feed 1 is 12 months from the date of manufacture. The expiration date is provided on the product label.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How often and at what concentration should I feed my CHO cells with Efficient-Pro AGT Feed 1?

The comprehensive feed protocol for Efficient-Pro AGT Feed 1 is included in the user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0025907_EfficientProAGTFeed1_UG.pdf). We recommend first designing a small-scale experiment using an Ambr15 Microbioreactor or other small vessel with concentrations between 1.5% to 3.0% of Efficient-Pro AGT Feed 1 in 0.5% increments beginning on day 3, with daily feeds through harvest. Once the specific feeding strategy is tailored to your CHO cell system for optimized results, cultures can be scaled up to larger vessels and bioreactors.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

When should I consider using Efficient-Pro AGT Feed 1?

Efficient-Pro AGT Feed 1 is a chemically-defined, animal-origin-free supplement that has been formulated to work specifically with CHO-S and DG-44 cells for production of proteins. Efficient-Pro AGT Feed 1 has been designed to work optimally when paired with Efficient-Pro AGT Medium (https://www.thermofisher.com/order/catalog/product/A5322301). Efficient-Pro AGT Feed 1 has been demonstrated to improve yield and other end-points when used as described in the user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0025907_EfficientProAGTFeed1_UG.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.