MagMAX™ Ultra 废水核酸分离和病毒富集试剂盒
MagMAX™ Ultra 废水核酸分离和病毒富集试剂盒
Applied Biosystems™

MagMAX™ Ultra 废水核酸分离和病毒富集试剂盒

MagMAX Ultra 废水核酸分离和病毒富集试剂盒可从废水样品中高效、高通量地进行病毒富集和核酸提取。该试剂盒采用 Dynabeads 和 MagMAX 磁珠技术,可确保以可重现的方式回收与多种应用兼容的高质量核酸,包括实时荧光定量 PCR了解更多信息
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货号数量
A52610100 Preps
货号 A52610
价格(CNY)
8,070.00
飞享价
Ends: 31-Dec-2025
9,263.00
共减 1,193.00 (13%)
Each
添加至购物车
数量:
100 Preps
价格(CNY)
8,070.00
飞享价
Ends: 31-Dec-2025
9,263.00
共减 1,193.00 (13%)
Each
添加至购物车
MagMAX Ultra 废水核酸分离和病毒富集试剂盒可从废水样品中高效、高通量地进行病毒富集和核酸提取。该试剂盒采用 Dynabeads 和 MagMAX 磁珠技术,可确保以可重现的方式回收与多种应用兼容的高质量核酸,包括实时荧光定量 PCR 和下一代测序。

MagMAX Ultra 废水核酸分离试剂盒的特点包括:
• 自动化浓缩和提取方案,设计用于 KingFisher Flex、KingFisher APEX 和 KingFisher Duo 系统
• 快速程序,可在 75 分钟内对 24 份废水样品进行病毒富集和核酸提取
• Dynabeads 废水病毒富集用于在提取前浓缩废水
• 洗脱体积为 50-100 µL

废水和污水监测是监测病毒和细菌病原体地区传播的有用工具。不同操作水平的监测(如大学集体宿舍或整个城市)已被证明是一种可靠方法,可在 SARS-CoV-2 病毒在人群中广泛传播之前对该病毒进行检测。废水监测可用于监测其他病毒靶标,如流感和诺如病毒。然而,废水和污水样品在提取 RNA 和 DNA 方面目前仍极具挑战性。

自动病毒富集
可处理最多 10 mL 废水,可进行病毒浓缩和提取。当使用 KingFisher Flex 或 APEX 系统进行病毒富集和提取流程时,可在仅 75 分钟内处理 24 份样品。方案完成后,将纯 RNA 和 DNA 浓缩于 50–100 µL 洗脱缓冲液中。如果需要超速离心或沉淀等其他浓缩方法,可使用 MagMAX Ultra 废水核酸分离试剂盒用于 200 µL 至 500 mL 上样量,该试剂盒仅包含提取试剂,使用户能够使用自选浓缩方法、过滤或非浓缩废水样品。

MagMAX 磁珠法纯化的优势
与其他分离核酸的技术相比,磁珠具备许多优势。相比玻璃纤维过滤柱,磁珠可以更有效地结合 DNA 和 RNA,获得更高、更一致的产量。此外,由于未使用过滤柱和真空歧管,不存在样品中的细胞颗粒堵塞过滤柱的风险。

MagMAX Ultra 废水核酸分离试剂盒中包含的 Dynabeads MyOne Silane 具有经过优化的类硅胶表面化学结构和高比表面积,可实现高效动力学和高灵敏度核酸捕获。上述特性使 Dynabeads MyOne Silane 成为回收 RNA 和 DNA 的理想选择。一旦捕获,这些核酸即可在 50–100 µL 洗脱缓冲液中洗脱,用于下游应用。
仅供科研使用。不可用于诊断程序。
规格
洗脱体积100 to 500 μL
最终产品类型RNA and DNA
适用于(应用)RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization
适用于(设备)KingFisher™ Flex,APEX,Duo
数量100 Preps
样品类型Wastewater
运输条件室温
原始材料量15 mL
测试时间Up to 45 min. when using automated protocol
分离技术磁珠
Unit SizeEach
内容与储存
• 10 mL Dynabeads Wastewater Virus Enrichment; 4°C
• 80 mL Lysis Buffer; room temperature
• 50 mL Binding Solution; room temperature
• 200 mL Wash Buffer; room temperature
• 25 mL Elution Solution; room temperature
• 4 mL Proteinase K; room temperature
• 2 mL DNA/RNA Binding Beads; room temperature

常见问题解答 (FAQ)

Is the purified viral nucleic acid that is obtained using MagMAX Wastewater Ultra Nucleic Acid Isolation Kit, with Virus Enrichment completely inhibitor-free?

The MagMAX Wastewater Ultra Nucleic Acid Isolation Kit, with Virus Enrichment allows elimination of the vast majority of diverse inhibitors of enzymatic reactions, even from the most diluted and challenging samples such as wastewater. In very rare instances, if some inhibitors still remain within the purified nucleic acid, the sample can be diluted 10-20X prior to downstream PCR or other applications.

What starting volume of wastewater can be used with the MagMAX Wastewater Ultra Nucleic Acid Isolation Kit, with Virus Enrichment?

The MagMAX Wastewater Ultra Nucleic Acid Isolation Kit, with Virus Enrichment is compatible with starting volumes of 1-10 mL. If concentration is not needed, or if wastewater has been concentrated using ultracentrifugation or precipitation, use the MagMAX Wastewater Ultra Nucleic Acid Isolation Kit (Cat. No. A52606).

How long is the protocol for the MagMAX Wastewater Ultra Nucleic Acid Isolation Kit, with Virus Enrichment?

The MagMAX Wastewater Ultra Nucleic Acid Isolation Kit, with Virus Enrichment protocol takes 90 mins to concentrate virus and isolate nucleic acid.

Is the MagMAX Wastewater Ultra Nucleic Acid Isolation Kit, with Virus Enrichment automation-compatible?

The MagMAX Wastewater Ultra Nucleic Acid Isolation Kit, with Virus Enrichment is compatible with the KingFisher Flex, APEX, and Duo instruments, and can also be used for manual processing in 24- and 96-well plates.