Ion AmpliSeq™ Polyploidy Panel

The Ion AmpliSeq Polyploidy Panel empowers accurate quality controls in preimplantation genetic testing for aneuploidy (PGT-A) by leveraging SNP-based assessments.

Benefits of the Ion AmpliSeq Polyploidy Panel include:
• Germline SNP detection from embryo biopsy samples—no parental DNA required, enabling the extraction of genetic insights directly from the sample
• Comprehensive genetic coverage—comprises 74 microhaplotype amplicons for human identification and 368 single SNP amplicons representing the population at large. With a total of 590 SNP sites covered, the panel unveils the unique genomic variations present in each sample, facilitating premium PGT-A analysis.
• Triploidy 69,XXX identification—through the utilization of tri-allelic microhaplotype sites and allele frequency distributions, samples with the potentially lethal triploidy 69,XXX can be confidently identified. This abnormality often appears similar to the normal 46,XX on conventional PGT-A plots, but the panel's advanced techniques help overcome this challenge.
• Mitigation of maternal contamination risk—reliable assessment of contamination from cumulus cell DNA made possible by tri-allelic microhaplotype sites. This assessment assists in prioritizing low-risk samples with minimized maternal contamination concerns.
• Sibling embryo tracking—SNP clustering allows for the identification of genetically related embryos, helping prevent sample mix-ups.

Enhance sample prioritization in PGT-A through the implementation of SNP-based quality controls
Single nucleotide polymorphisms (SNPs) are unique genetic variations that can be inherited and directly associated with parental DNA. By leveraging SNPs, PGT-A analysis gains valuable insights into triploidy 69,XXX, maternal contamination, and embryo identity, helping ease the process of embryo selection for IVF research.

Notes
• This panel is compatible with both the Ion GeneStudio and Genexus systems.