TaqMan™ QSY 探针
TaqMan™ QSY 探针
Applied Biosystems™

TaqMan™ QSY 探针

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Applied Biosystems TaqMan QSY 探针是用于实时荧光定量 PCR 的双标记探针。TaqMan QSY 探针可为多重了解更多信息
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货号产品类型数量
A56899QSY2 Probe6000 pmole
4482777QSY 探头6,000 pmol
4482778QSY 探头20,000 pmol
4482779QSY 探头50,000 pmol
A56900QSY2 Probe20,000 pmol
A56901QSY2 Probe50,000 pmol
货号 A56899
价格（CNY)
3,002.00
Each
立刻订购
产品类型:
QSY2 Probe
数量:
6000 pmole
Applied Biosystems TaqMan QSY 探针是用于实时荧光定量 PCR 的双标记探针。TaqMan QSY 探针可为多重 qPCR 实验提供卓越的灵敏度和性能。诸如黑洞淬灭基团（Black Hole Quencher，以下简称 BHQ™）的暗淬灭探针的用户可以设计自定义 QSY 探针，而无需更改探针序列。

所有 TaqMan QSY 探针均经过 HPLC 纯化，并已通过质谱法验证，可确保各批间批次间的重现性。20多年来，Thermo Fisher Scientific 始终是 qPCR 探针制造领域的领导者。精通于在经过 ISO 13485 认证的设施中合称探针。

TaqMan QSY 探针的特点包括：
•灵敏度—因背景信号低和信号增强具有较好的灵敏度
• 具有良好的多重测定性能—只需一次反应即可检测多重多个基因靶点
• 有多种染料可供选择，是 Applied Biosystems qPCR 仪器滤波器的理想选择—TaqMan qPCR 染料与 Applied Biosystems qPCR 仪器高度匹配，能改善荧光信号，尤其是低表达基因靶标
• 灵活性—在单次反应中，使您的多重 qPCR 探针能与多达2种 MGB 探针或4种 QSY 探针进行混合并匹配
• 快速启动—BHQ 探针序列可以转换为 QSY 探针，无需重新设计
性能不受影响
使用 TaqMan QSY 探针进行多重测定可节约成本和有限的样本，已证明定量检测基因时，单管反应与4重反应产生的结果相当。

了解更多关于多重实时荧光定量 PCR 的信息›

保证收率
根据提供的最低量来选择所需单位规格，而非根据合成起始量。

预计运输时间
北美4–6天、欧洲5-10天

。我们还提供 Applied Biosystems 定制引物和其他类型的 TaqMan 探针。了解更多 ›

需要不同染料或浓度或更大批次吗？TaqMan 定制检测试剂盒和寡核苷酸服务提供定制产品，可满足您的实验需求。了解更多 ›

仅供研究使用。
规格
3' 引物修改QSY 2
5' 引物修改Cyanine 5, Cyanine 5.5
适用于（设备）7500 系统、7500 Fast 系统、7900HT 系统、StepOne™、StepOnePlus™、ViiA™ 7 系统、QuantStudio™ 3、QuantStudio™ 5、QuantStudio™ 6 Flex、QuantStudio™ 7 Flex、QuantStudio 6 Pro、QuantStudio 7 Pro、QuantStudio™ 12k Flex
产品规格管装
环保功能Sustainable packaging
内部探针修改无
产品线TaqMan™
产品类型QSY2 Probe
纯化方法HPLC
数量6000 pmole
运输条件Room Temperature
检测方法引物-探针
形式液体或干燥
PCR 方法qPCR
反应速度快速
Unit SizeEach
内容与储存
1 tube containing a dual-labeled probe in solution or dried down. Store at –15°C to –25°C.

常见问题解答 (FAQ)

I need to see all the primer and probe sequences from an assay design right away. How can I do this?

You can directly see all the primer and probe sequences from a test design right away using the Primer Express Software. Open the software and choose File => New. Choose either TaqMan MGB Quantification or TaqMan Quantification for Gene Expression Assay design. Copy/paste your gene sequence of interest into the new window and click on the green triangle to get primer/probe designs.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

How can I determine whether the Tm of my custom probe is appropriate?

In general, the target Tm of a TaqMan probe is 70 degrees C. Using the Primer Express Software, you can check the Tm of a MGB or TAMRA probe probe.

Within the software, go to Primer Probe Test Tool and then copy/paste in your sequence of interest to see the Tm. If you need to check for a MGB based probe, make sure to choose Document Type: TaqMan MGB Quantification. If you need to check for a QSY or TAMRA based probe, make sure to choose Document Type: TaqMan Quantification. Real-Time PCR and Digital PCR Applications

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

Do I need to redesign my probe when converting from BHQ and other competitor probes to TaqMan QSY/QSY2 Probes?

No, redesign is not necessary when converting from most competitor probes to TaqMan QSY/QSY2 Probes.

Find additional tips, troubleshooting help, and resources within our TaqMan Primers and Probes Support Center.

Is there a need to adjust filter settings on the instrument to utilize Cyanine 5 and Cyanine 5 dyes with TaqMan QSY/QSY2 Probes?

Yes, please make sure to enable the x6m6 filter (6th channel) in the instrument filter settings prior to the qPCR run. This filter is not preselected by default.

Find additional tips, troubleshooting help, and resources within our TaqMan Primers and Probes Support Center.

If I am using Cyanine 5 dye with TaqMan QSY/QSY2 Probes, can I still use Mustang Purple dye as the passive reference dye?

No, these dyes are incompatible as they utilize the same filter set.

Find additional tips, troubleshooting help, and resources within our TaqMan Primers and Probes Support Center.

View all TaqMan™ QSY 探针 FAQs