How long can 10X DNA working panel FWD and REV subpools be stored?
10X DNA working panel FWD and REV subpools can be stored at 4 degrees C for one week. For longer term storage, aliquot and store subpools at -20 degrees C.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
Have there been any recent changes to the protocol for Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283)?
There have been some recent changes to the protocol. Please refer to the latest user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017392_Ion%20AmpliSeqHDLibraryKit_UG.pdf) for full details.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
When should I use CRC versus HD Enhancer for Ion AmpliSeq HD library preparation?
CRC is a component of the Ion AmpliSeq HD Library Kit (Cat. No. A37694). CRC improves target amplification reactions for challenging panels that produce excessive amounts of primer dimer or panels that have ⋝500 primer pairs. We recommend using CRC with all panel types. HD Enhancer is a component of the Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283) and is also available separately as Cat. No. A53690. HD Enhancer is used for improved library quality and molecular coverage results. Guidelines for using HD Enhancer or CRC for Ion AmpliSeq HD library preparation are outlined in the user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017392_Ion%20AmpliSeqHDLibraryKit_UG.pdf).
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
Which barcodes are compatible with Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283)?
We recommend using the Ion AmpliSeq HD Dual Barcode Kit 1-24 (Cat. No. A37695) with Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283). The libraries are dual barcoded at the 5' and 3' ends and this enables the multiplexing of up to 24 samples in a single chip. Using these dual barcodes also reduces the risk of barcode cross-contamination.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
What are the shipping and storage conditions for Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283)?
Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283) is shipped on dry ice and we recommend storing it at -20 degrees C.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.