• 160 mL 结合溶液 • 300 mL洗涤 I 溶液 • 6 mL 结合磁珠 • 3 mL 蛋白酶 K • 36 mL 洗脱缓冲液 • 30 μL 蛋白酶 K 染料
在 15 – 25°C 下储存
引用和文献 (4)
引用和文献
Abstract
Evaluation of Self-Collected Mouth Rinse Specimens for Coronavirus Disease 2019 Testing: A Pilot Study
Authors:Kento Fukano; Junko S. Takeuchi; Azusa Kamikawa; Wataru Sugiura
Journal:COVID
PubMed ID:
Saliva specimens are widely used for coronavirus disease 2019 (COVID-19) testing using RT-qPCR due to their advantages over nasopharyngeal swabs of being non-invasive and self-collectable. However, saliva collection can be time-consuming in individuals with reduced saliva secretion, including those with diabetes, diseases involving salivary glands such as Sjögren’s syndrome, and ... More
Interdependent RNA structural motifs at the 3'-terminus of the West Nile virus genome regulate viral growth.
Authors:Tsao LH,Brackney DE,Pyle AM
Journal:bioRxiv : the preprint server for biology
PubMed ID:41959387
The RNA genome of West Nile Virus (WNV) folds into an elaborate series of RNA structural elements that are crucial for viral function. Among these elements, four pseudoknots (PKs) at the viral 3’-terminus, designated as SLII, SLIV, DBI, and DBII, are among the most crucial players in the overall flaviviral ... More
Saliva as a Reliable and Non-invasive Sample for Detecting Influenza A in Severe Acute Respiratory Infection Cases.
Background Nasopharyngeal swab sampling remains the gold standard for influenza diagnosis; however, it has several limitations, including dependence on medical staff, invasiveness, potential for nosocomial transmission, and occupational exposure risk. Non-invasive alternatives, such as saliva and nasal vestibular swabs, may improve patient comfort and participation in clinical studies. In addition, ... More
Bead-based approaches for increased sensitivity and multiplexing of CRISPR diagnostics.
CRISPR-based diagnostics have emerged as a promising tool for fast, accurate and portable pathogen detection. There has been rapid progress in pre-amplification processes and CRISPR-related enzymes used in these approaches, but the development of reporter systems and reaction platforms has lagged behind. In this paper, we develop bead-based techniques to ... More