MagMAX Pro HT NoSpin 质粒小量制备试剂盒

Applied Biosystems™

MagMAX Pro HT NoSpin 质粒小量制备试剂盒

Name: MagMAX Pro HT NoSpin 质粒小量制备试剂盒
SKU: A58309
Price: 2729.00 CNY

MagMAX™ Pro HT NoSpin 质粒小量制备试剂盒可实现从细菌培养物中可扩展、超快速地纯化高质量转染级质粒 DNA (pDNA)。该试剂盒采用磁珠技术，消除了离心和真空过滤的瓶颈，从而简化工作流程。

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货号	反应次数
A58309	96 次反应
A58310	384 次反应
A58311	1152 次反应
A58312	2304 次反应

4 选项

货号 A58309

价格（CNY)

2,729.00

Each

添加至购物车

反应次数:

96 次反应

价格（CNY)

2,729.00

Each

添加至购物车

Applied Biosystems MagMAX Pro HT NoSpin 质粒小量制备试剂盒为直接从细菌培养物中纯化高质量转染级质粒 DNA (pDNA) 提供了一种可扩展的超快速方法。MagMAX Pro HT NoSpin 试剂盒采用先进的磁珠技术，通过消除离心和真空过滤瓶颈，大幅简化和优化质粒纯化工作流程。

MagMAX Pro HT NoSpin 质粒小量制备试剂盒的特点包括：

利用双顺磁性磁珠进行质粒分离，无需耗时的处理步骤（离心、真空过滤）
提供现成的自动化操作的实验方案和程序，可在 KingFisher Apex 和 Flex 系统上于 35 分钟内处理 96 份样本
分离 6–12 Kb 大小的质粒
与常用培养基兼容
高质量 pDNA 产量高达 20μ g
适合转染的 pDNA，内毒素水平低至 <1 EU/μg

MagMAX Pro HT NoSpin 技术专为高通量质粒分离而设计，便于自动化操作，适合与 KingFisher Apex、KingFisher Flex 和 KingFisher Presto 纯化系统以及集成液体处理机器人一起使用。

MagMAX Pro HT 纯化化学试剂既有效又环保。试剂盒包括一种新型的内毒素去除缓冲液，用于去除热原污染物，缓冲液不含乙醇和变性盐。

使用 MagMAX Pro HT NoSpin 试剂盒纯化的 pDNA 适用于广泛的下游应用，如转染、克隆、酶切、体外转录和测序。

规格

洗脱体积100 μL

适用于（应用）克隆、转染、蛋白表达、蛋白生产、通过体外转录生产 mRNA、基因编辑、测序、PCR、核酸标记、PCR

适用于（设备）KingFisher Apex、Flex 和 Presto 纯化系统，配备 96 深孔板磁头，液体处理机器人系统

环保功能节能设计、减少包装、无需挥发性有机溶剂

反应次数96 次反应

核酸类型质粒 DNA (pDNA)

板兼容性KingFisher 96 深孔板（带或不带条形码）

产品类型自动化 pDNA 提取试剂盒

纯度转染级

数量1 Ea.

样品类型细菌，大肠杆菌

样品体积细菌培养物，每孔 1 mL

规格小量

规格分布5-12kb

测试时间96 个样品约需 32 分钟，1 个 KingFisher 96 深孔板

产量使用高拷贝质粒，高达 20μ g pDNA

产品规格96 孔深孔板

分离技术磁珠法

Unit SizeEach

内容与储存

2°C 至 8°C BactBind 磁珠、PlasmidPure 磁珠、内毒素去除缓冲液
15°C 至 25°C RNAse A、重悬缓冲液、裂解缓冲液、沉淀缓冲液、清洗 1 缓冲液、清洗 2 缓冲液、洗脱缓冲液

常见问题解答 (FAQ)

Can I grow an overnight bacterial culture directly in the KingFisher 96-well plate when using the MagMAX Pro HT NoSpin Plasmid MiniPrep Kit?

Yes, the KingFisher 96-well plate can be used for overnight bacterial culture growth with the MagMAX Pro HT NoSpin Plasmid MiniPrep Kit. However, growth conditions depend on factors such as media type, host strain, plasmid copy number, and plasmid size. For best results, optimize orbital shaker settings and monitor OD600 to minimize well-to-well variation and ensure consistent plasmid yield.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

How does plasmid copy number affect yields with the MagMAX™ Pro HT NoSpin Plasmid MiniPrep Kit?

High-copy plasmids yield higher amounts of DNA and are ideal for most downstream applications. Low-copy plasmids can be extracted, but yields may be lower and may not be sufficient for all transfection protocols. For low-copy plasmids, optimizing culture conditions, increasing culture volume, or performing multiple elution steps may help improve yield.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Which bacterial strains are compatible with the MagMAX Pro HT NoSpin Plasmid MiniPrep Kit?

The MagMAX Pro HT NoSpin Plasmid MiniPrep Kit is compatible with K12 E. coli derivatives, including DH5alpha, TOP10, and DH10B strains. Plasmid yield may vary with low-copy origins, strong host dependencies, or high genomic integration tendencies.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Should I be concerned about bead clumps in the BactBind Beads or PlasmidPure Beads bottles before use?

No, bead settling during transit is normal. This does not affect performance. We recommend vortexing well, before use.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What should I do if there are precipitates in the Lysis Buffer within the MagMAX Pro HT NoSpin Plasmid MiniPrep Kit?

We recommend warming the buffer at 30-37 degrees C for 30 min, and then inverting to mix until the precipitates dissolve.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.