TaqMan™ Cells-to-CT™ Express 1-Step Kit

Skip extraction entirely. Go from cell lysate to results in 1 step.

The TaqMan™ Cells-to-CT™ Express 1-Step Kit enables gene expression analysis directly from cultured cell lysates in a single RT-qPCR reaction. By eliminating RNA purification and combining reverse transcription and quantitative PCR into one streamlined workflow, researchers can generate high-quality gene expression data with fewer processing steps and less hands-on time. The workflow is well suited for early-stage research applications, including target identification, target validation, pathway analysis, and high-throughput screening.

Designed for speed and agility, the workflow combines rapid 5-minute cell lysis with integrated 1-step RT-qPCR, allowing researchers to move from cells to gene expression results in approximately 70 minutes.

Simplify RT-qPCR Workflows for Early Target Validation Research

RT-qPCR remains one of the most widely used methods for measuring gene expression during early-stage drug discovery and biological research. However, traditional workflows typically require RNA purification before amplification, adding time, consumables, and multiple handling steps to the process.

Direct-lysis workflows offer an alternative by enabling cells to be processed and analyzed without RNA extraction. When paired with 1-step RT-qPCR, researchers can move seamlessly from sample preparation to gene expression analysis in a single, continuous workflow.

The TaqMan™ Cells-to-CT™ Express 1-Step Kit was developed to support laboratories performing gene expression-based screening and target validation studies, helping reduce workflow complexity while maintaining the sensitivity, reproducibility, and performance required for confident decision-making.

Key Features & Benefits

• Simplified Gene Expression Workflow – Go directly from cultured mammalian cells to RT-qPCR with no RNA purification required and no separate reverse transcription reaction required. Rapid 5-minute cell lysis with no stop solution or heat inactivation step further streamlines sample preparation.
• Reduced Sample Handling – Minimize pipetting and sample transfer steps to reduce opportunities for user error, contamination, and sample loss.
• Lower Plastic and Consumable Usage – Reduce the number of tubes, plates, pipette tips, and reagents required compared to traditional extraction-based workflows.
• Reliable Gene Expression Results – Generate sensitive and reproducible gene expression data directly from cultured cell lysates using trusted TaqMan™ Gene Expression Assays.
• Scalable for High-Throughput Workflows – Compatible with both manual and automated laboratory environments, featuring an automation-ready workflow that enables efficient processing of large numbers of samples.
• Designed for Early Discovery Research – Enable gene expression analysis for target identification, target validation, and screening applications.

Applications

The TaqMan™ Cells-to-CT™Express 1-Step Kit is designed for RT-qPCR-based gene expression workflows in research and early drug discovery screening labs.

Applications include:

• RT-qPCR gene expression analysis
• Target identification and gene expression-based target validation
• Early-stage drug discovery screening
• High-throughput compound screening
• Pathway activation and response studies
• RNA analysis from cultured mammalian cells
• Candidate target prioritization
• Biomarker and gene expression profiling
• RNA-mediated gene regulation studies