When should I use a Thermo Scientific low profile PCR plastic?
We recommend Thermo Scientific low profile PCR plates and tubes for reaction volumes below 20 µL. The low profile design minimizes the airspace between the PCR reaction and reduces evaporation effects, thereby increasing PCR efficiency in low volume reactions.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
What is a low profile Thermo Scientific plate/tube?
Low profile Thermo Scientific PCR plastics have shorter wells than the standard profile plastics, decreasing the “dead space” between the heated lid of the thermal cycler and the sample. This eliminates condensation on the side wall of the tube, preventing reduction in PCR volume and increasing the efficiency of the reaction.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
Plate warping is causing problems with the robot in my automated processes. How can I solve this?
We recommend Thermo Scientific Armadillo PCR plates which are designed for robotic handling with high rigidity and resistance. Combining the rigidity of a polycarbonate frame with thin walled polypropylene wells, the Armadillo plates provide superior thermal cycling performance under all conditions without warping.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
Are Thermo Scientific ABgene plastics endotoxin-free?
They are not certified endotoxin-free. However, their entire production process, from molding to final packaging, is carried out in a Class 100,000 clean room under ISO 9001 guidelines. All Thermo Scientific PCR plastics are certified free from RNase, DNase and human DNA.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
Are Thermo Scientific ABgene plastics sterile?
Thermo Scientific Abgene plastics are not typically sterilized as their entire production process, from molding to final packaging, is carried out in a Class 100,000 clean room under ISO 9001 guidelines. All Thermo Scientific PCR plastics are certified free from RNase, DNase and human DNA. In contrast, during typical non-clean room production, plastics are exposed to many contaminants including dust, bacterial cells, and DNA. The plastics are then sterilized to kill bacteria and inactivate RNases and DNases, but sterilization does not remove dust or DNA contamination. The dust particles left behind can inhibit PCR, and the damaged DNA fragments can still act as templates, leading to non-specific amplification.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
