BCECF 酸 (2',7'-双-(2-羧乙基)-5-(和-6)-羧基荧光素)，混合异构体

引用和文献 (84)

Invitrogen™

BCECF 酸 (2',7'-双-(2-羧乙基)-5-(和-6)-羧基荧光素)，混合异构体

因此，BCECF 适用于测量 pH 值7附近的 ± 1 pH 单位的变化，因为它是一种 pKa 为了解更多信息

货号	数量
B1151 又称 B-1151	1 mg

货号 B1151

又称 B-1151

价格（CNY)

3,182.00

添加至购物车

价格（CNY)

3,182.00

添加至购物车

因此，BCECF 适用于测量 pH 值7附近的 ± 1 pH 单位的变化，因为它是一种 pK_a 为 ∼6.98 的双激发比率式 pH 值指示剂。通过染料在 ∼490 nm 处激发的发射强度（535 nm 处测得）与在 ∼440 nm 处等吸光点激发的发射强度的比例（pH 值依赖性），利用 BCECF 完成 pH 值测量。

了解有关离子指示剂（包括钙、钾、pH 值和膜电位指示剂）的更多信息›

仅供科研使用。不可用于诊断程序。

检测方法荧光

染料类型pH 值指示剂

数量1 mg

运输条件室温

适用于（设备）微孔板读数仪

产品类型BCECF 酸性 pH 值指示剂

Unit SizeEach

内容与储存

室温避光储存。

引用和文献 (84)

引用和文献

Abstract

Measurement of intracellular pH.

Authors:Boyer MJ, Hedley DW

Journal:Methods Cell Biol

PubMed ID:7532259

Fluorescent indicators of ion concentrations.

Authors:Tsien RY

Journal:Methods Cell Biol

PubMed ID:2538708

Mechanistic studies of lantibiotic-induced permeabilization of phospholipid vesicles.

Authors:Driessen AJ, van den Hooven HW, Kuiper W, van de Kamp M, Sahl HG, Konings RN, Konings WN

Journal:Biochemistry

PubMed ID:7849020

'Nisin is a cationic polycyclic bacteriocin secreted by some lactic acid bacteria. Nisin has previously been shown to permeabilize liposomes. The interaction of nisin was analyzed with liposomes prepared of the zwitterionic phosphatidylcholine (PC) and the anionic phosphatidylglycerol (PG). Nisin induces the release of 6-carboxyfluorescein and other small anionic fluorescent ... More

Luminal pH regulated calcium release kinetics in sarcoplasmic reticulum vesicles.

Authors:Donoso P, Beltrán M, Hidalgo C

Journal:Biochemistry

PubMed ID:8873610

'Calcium binding to triads isolated from rabbit skeletal muscle followed a single hyperbolic function in the pH range 5.5-8.0. Maximal binding was obtained at pH 8.0; decreasing the pH decreased the binding capacity and, at pH < or = 6.0, increased Kd 2-fold. These results indicate that lowering the pH ... More

Modulation of the hyperpolarization-activated cation current of rat thalamic relay neurones by intracellular pH.

Authors:Munsch T, Pape HC

Journal:J Physiol

PubMed ID:10457065

'1. Properties of the hyperpolarization-activated cation current (Ih) were investigated in thalamocortical neurones of an in vitro slice preparation of the rat ventrobasal thalamic complex (VB) before and during changes of pipette pH (pHp), intracellular pH (pHi) and bath pH (pHb) using the whole-cell patch-clamp technique and fluorescence ratio imaging ... More

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