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引用和文献 (233)
Invitrogen™
Bis-ANS（4,4'-二苯胺基-1,1'-联萘-5,5'-二磺酸二钾盐）
Bis-ANS对于蛋白质的非极性空穴的结合能力高于 1-8-ANS，结合亲和力往往比后者高出几个数量级。苯胺基萘磺酸 (ANS) 在水中基本不发荧光，仅在与膜结合后才会发出明显的荧光。这一性质使其成为蛋白质折叠、构象变化以及其他因探针接触水而改性的蛋白质过程的灵敏指示剂。了解更多信息
| 货号 | 数量 |
|---|---|
| B153 | 10 mg |
货号 B153
价格（CNY)
5,374.00
Each
数量:
10 mg
价格（CNY)
5,374.00
Each
Bis-ANS对于蛋白质的非极性空穴的结合能力高于 1-8-ANS，结合亲和力往往比后者高出几个数量级。苯胺基萘磺酸 (ANS) 在水中基本不发荧光，仅在与膜结合后才会发出明显的荧光。这一性质使其成为蛋白质折叠、构象变化以及其他因探针接触水而改性的蛋白质过程的灵敏指示剂。
仅供科研使用。不可用于诊断程序。
规格
标签类型其他标记或染料
分子式C32H22K2N2O6S2
产品线Molecular Probes
数量10 mg
建议的储存条件室温避光储存。
运输条件室温
子细胞定位细胞膜&脂质
物理形态实心
产品类型Bis-ANS
Unit SizeEach
引用和文献 (233)
引用和文献
Abstract
Interaction of 1,1'-bi(4-anilino)naphthalene-5,5'-disulfonic acid with alpha-crystallin.
Journal:J Biol Chem
PubMed ID:9535881
'The hydrophobic sites in alpha-crystallin were evaluated using a fluorescent probe 1,1''-bi(4-anilino)naphthalenesulfonic acid (bis-ANS). Approximately one binding site/subunit of alpha-crystallin at 25 degrees C was estimated by equilibrium binding and Scatchard analysis (Kd = 1.1 microM). Based on fluorescence titration, the dissociation constant was 0.95 microM. The number of bis-ANS
Plurality of protein conformations of ribulose-1,5-bisphosphate carboxylase/oxygenase monomers probed by high pressure electrophoresis.
Journal:J Biol Chem
PubMed ID:7902356
'We used hydrostatic pressure in the range of 1 to 2 kbar, coupled with polyacrylamide gel electrophoresis, to investigate the properties of monomers of dimeric ribulose bisphosphate carboxylase/oxygenase. At temperatures below -5 degrees C or pressures above 1.5 kbar, only a diffuse band with low electrophoretic mobility was observed, which
An operator-induced conformational change in the C-terminal domain of the lambda repressor.
Journal:J Biol Chem
PubMed ID:1532575
'4,4''-bis(1-anilino-8-naphthalenesulfonic acid (Bis-ANS), an environment-sensitive fluorescent probe for hydrophobic region of proteins, binds specifically to the C-terminal domain of lambda repressor. The binding is characterized by positive cooperativity, the magnitude of which is dependent on protein concentration in the concentration range where dimeric repressor aggregates to a tetramer. In this
Exploiting new potential targets for anti-hepatitis B virus drugs.
Journal:Curr Drug Targets Infect Disord
PubMed ID:14529356
'Based on the recent studies of HBV strains with different replication efficiency, several new potential targets for anti-HBV replication have been presented. These include the viral and cellular regulatory factors associated with HBV replication and the process for encapsidation of viral genome and budding into endoplasmic reticulum (ER). A putative
Detection of disulfide bonds in bovine brain tubulin and their role in protein folding and microtubule assembly in vitro: a novel disulfide detection approach.
Journal:Biochemistry
PubMed ID:11467944
'Cysteine residues in tubulin are actively involved in regulating ligand interactions and microtubule formation both in vivo and in vitro. These cysteine residues are sensitive reporters in determining the conformation of tubulin. Although some of the cysteines are critical in modulating drug binding and microtubule assembly, it is not clear