BacLight™ Green 细菌染色剂
<i>Bac</i>Light&trade; Green 细菌染色剂
Invitrogen™

BacLight™ Green 细菌染色剂

BacLight Green(货号 B-35000)和 BacLight Red(货号 B-35001)细菌染色剂是用于检测和监测细菌的荧光标记试剂了解更多信息
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货号数量
B3500020 x 50 μg
货号 B35000
价格(CNY)
3,794.00
飞享价
Ends: 31-Dec-2025
5,041.00
共减 1,247.00 (25%)
Each
添加至购物车
数量:
20 x 50 μg
价格(CNY)
3,794.00
飞享价
Ends: 31-Dec-2025
5,041.00
共减 1,247.00 (25%)
Each
添加至购物车
BacLight Green(货号 B-35000)和 BacLight Red(货号 B-35001)细菌染色剂是用于检测和监测细菌的荧光标记试剂。这两种染料不是核酸染色剂。用 BacLight Green 和 BacLight Red 细菌染色剂染色的细菌可发出明亮的绿色和红色荧光(吸收/发射波长分别为 ∼480/516 和 ∼581/644 nm),并可使用适当的流式细胞通道或荧光显微镜检查滤光片分离。BacLight 细菌染色剂与甲醛或乙醇固定法兼容。

查看有关所有微生物分析产品的更多信息。
仅供科研使用。不可用于诊断程序。
规格
细胞类型细菌
检测方法荧光
染料类型其他标记或染料
形式实心
产品规格管、玻片
数量20 x 50 μg
运输条件室温
溶解度DMSO(二甲亚砜)
发射480⁄ 561
适用于(设备)荧光显微镜, 流式细胞仪
产品线BacLight
产品类型细菌染色剂
Unit SizeEach
内容与储存
含 20 瓶 BacLight™ Green 细菌染色剂(每瓶 50 μg)。 在冰箱(-5 至 -30°C)中避光储存。

常见问题解答 (FAQ)

我可以在流式细胞仪上看到哪些细菌参数?

您可以用BacLight Green细菌染色剂(货号B35000)或BacLight Red细菌染色剂(货号B35001)等常规染色剂进行细菌染色。你们可以看到革兰氏特性(货号L7005),细胞存活率(货号L7007、L7012和L13152),细胞计数(货号L34856和B7277)和细胞活力。细胞活力可以通过膜电位法(货号B34950)或代谢法(货号B34954和B34956)测定。

What bacterial parameters can I look at by flow cytometry?

You can stain bacteria with a general stain such as BacLight Green Bacterial Stain (Cat. No. B35000) or BacLight Red Bacterial Stain (Cat. No. B35001). You can look at gram character (Cat. No. L7005), cell viability (Cat. Nos. L7007, L7012, and L13152), cell count (Cat. Nos. L34856 and B7277), and cell vitality. Cell vitality can be measured by membrane potential (Cat. No. B34950) or by metabolism (Cat. Nos. B34954 and B34956).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What optical filter do your recommend with the BacLight Green Bacterial Stain?

We recommend filters suitable for fluorescein/FITC with the BacLight Green kit.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (4)

引用和文献
Abstract
Identification of broadly protective human antibodies to Pseudomonas aeruginosa exopolysaccharide Psl by phenotypic screening.
Authors:DiGiandomenico A, Warrener P, Hamilton M, Guillard S, Ravn P, Minter R, Camara MM, Venkatraman V, Macgill RS, Lin J, Wang Q, Keller AE, Bonnell JC, Tomich M, Jermutus L, McCarthy MP, Melnick DA, Suzich JA, Stover CK,
Journal:J Exp Med
PubMed ID:22734046
Pseudomonas aeruginosa is a leading cause of hospital-associated infections in the seriously ill, and the primary agent of chronic lung infections in cystic fibrosis patients. A major obstacle to effective control of P. aeruginosa infections is its intrinsic resistance to most antibiotic classes, which results from chromosomally encoded drug-efflux systems ... More
Analysis of adherence, biofilm formation and cytotoxicity suggests a greater virulence potential of Gardnerella vaginalis relative to other bacterial-vaginosis-associated anaerobes.
Authors:Patterson JL, Stull-Lane A, Girerd PH, Jefferson KK,
Journal:Microbiology
PubMed ID:19910411
'Worldwide, bacterial vaginosis (BV) is the most common vaginal disorder in women of childbearing age. BV is characterized by a dramatic shift in the vaginal microflora, involving a relative decrease in lactobacilli, and a proliferation of anaerobes. In most cases of BV, the predominant bacterial species found is Gardnerella vaginalis. ... More
Teleost skin, an ancient mucosal surface that elicits gut-like immune responses.
Authors:Xu Z, Parra D, Gómez D, Salinas I, Zhang YA, von Gersdorff Jørgensen L, Heinecke RD, Buchmann K, LaPatra S, Sunyer JO,
Journal:
PubMed ID:23884653
'Skin homeostasis is critical to preserve animal integrity. Although the skin of most vertebrates is known to contain a skin-associated lymphoid tissue (SALT), very little is known about skin B-cell responses as well as their evolutionary origins. Teleost fish represent the most ancient bony vertebrates containing a SALT. Due to ... More
SadA, a trimeric autotransporter from Salmonella enterica serovar Typhimurium, can promote biofilm formation and provides limited protection against infection.
Authors:Raghunathan D, Wells TJ, Morris FC, Shaw RK, Bobat S, Peters SE, Paterson GK, Jensen KT, Leyton DL, Blair JM, Browning DF, Pravin J, Flores-Langarica A, Hitchcock JR, Moraes CT, Piazza RM, Maskell DJ, Webber MA, May RC, MacLennan CA, Piddock LJ, Cunningham AF, Henderson IR,
Journal:Infect Immun
PubMed ID:21859856
Salmonella enterica is a major cause of morbidity worldwide and mortality in children and immunocompromised individuals in sub-Saharan Africa. Outer membrane proteins of Salmonella are of significance because they are at the interface between the pathogen and the host, they can contribute to adherence, colonization, and virulence, and they are ... More